|
US$889.00 · In stock
Delivery: <= 6 days. True-PDF full-copy in English will be manually translated and delivered via email.
HYT140-2011: Specifications for the survey of marine microbenthos
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| HY/T 140-2011 | English | 889 |
Add to Cart
|
6 days [Need to translate]
|
Specifications for the survey of marine microbenthos
| Valid |
HY/T 140-2011
|
PDF similar to HYT140-2011
Basic data | Standard ID | HY/T 140-2011 (HY/T140-2011) | | Description (Translated English) | Specifications for the survey of marine microbenthos | | Sector / Industry | Marine Industry Standard (Recommended) | | Classification of Chinese Standard | A45 | | Classification of International Standard | 7.06 | | Word Count Estimation | 36,388 | | Date of Issue | 5/9/2011 | | Date of Implementation | 6/1/2011 | | Quoted Standard | GB/T 12763.1; GB/T 12763.6, | | Regulation (derived from) | State-Standard-Committee2011 No. 6 (Total No. 138) | | Issuing agency(ies) | State Oceanic Administration | | Summary | This standard specifies the basic requirements of marine benthic micro-survey sampling and established fixed, isolated and cultured, centrifugation, and the general provisions and methods of extraction, dyeing and producer, qualitative and quantitative analysis. This standard applies to the intertidal zone, shallow water and deep-sea habitats of different marine benthic survey miniature. | HYT140-2011: Specifications for the survey of marine microbenthos---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Specifications for the survey of marine microbenthos
ICS 07.060
A45
People's Republic of China Marine Industry Standard
Marine microbenthic survey specifications
Released on.2011-05-09
2011-06-01 implementation
Issued by the State Oceanic Administration
Table of contents
Preface Ⅲ
Introduction Ⅳ
1 Scope 1
2 Normative references 1
3 Terms and definitions 1
4 Basic requirements 1
4.1 Main Observation 1
4.2 Auxiliary Observation 1
4.3 Observation site and hierarchical design 1
4.4 Documents and drawings 2
4.5 Technical requirements and procedural steps for investigation 2
4.6 Quality plan and quality control measures 2
5 Sampling and fixing 2
5.1 Sampling at sea 2
5.2 Intertidal sampling 3
5.3 Collection of living samples 3
5.4 Sampling tube 3
5.5 Sample processing 3
5.6 Determination of environmental factors 3
6 Investigation of living microbenthos 4
6.1 Method overview 4
6.2 Equipment and materials 4
6.3 Sample separation and cultivation 4
6.4 Observation and recording analysis 5
7 Investigation of Benthic Microalgae and Benthic Bacteria 5
7.1 Method overview 5
7.2 Fluorescence staining for laboratory operations 5
7.3 Fluorescent staining for shipboard operations 7
8 Benthic protozoa survey 8
8.1 Method overview 8
8.2 Tools and reagents 8
8.3 Percol centrifugal separation technology 8
8.4 Ludox centrifugal separation technology 9
8.5 Quantitative protein silver staining technique 10
9 Qualitative and quantitative analysis of microbenthos 13
9.1 Microscopic examination and analysis 13
9.2 Calculation of common community parameters 13
9.3 Fill in the report 14
9.4 Statistical analysis and graphing 14
10 Contents and requirements of survey results 14
10.1 Original record 14
10.2 Documentation 15
10.3 Metadata 16
10.4 Figure 17
10.5 Investigation conclusion 17
10.6 Quality assessment of results 17
Appendix A (Normative Appendix) Several main groups of microbenthos Figure 18
Appendix B (Informative Appendix) Record Form of Microbenthos Sampling and Microscopy 19
Appendix C (Normative Appendix) Several instruments and equipment for microbenthic survey Figure 21
Appendix D (Normative Appendix) Table 22 of the biomass conversion coefficients of microbenthos
Appendix E (Normative Appendix) Flow chart of several key techniques for microbenthic survey 23
Appendix F (Normative Appendix) The biological volume calculation formula of microbenthos 25
Appendix G (Normative Appendix) The formulation and preparation of various solutions 27
References 29
Figure A.1 Several main groups of microbenthos Figure 18
Figure C.1 Vacuum filtration system Figure 21
Figure C.2 Filter basket Figure 21
Figure E.1 Flow chart of fluorescent staining technique for benthic microalgae and bacteria 23
Figure E.2 Flow chart of Percol centrifugal separation technology 23
Figure E.3 Ludox centrifugal separation technology flow chart 24
Figure E.4 Flow chart of quantitative protein silver staining (QPS) technique 24
Figure F.1 The biological volume calculation formula of microbenthos Figure 25
Table B.1 Microbenthic Sampling Record Table 19
Table B.2 Microscopic examination record of microbenthos 20
Table D.1 Biomass conversion coefficient of microbenthos Table 22
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that certain contents of this document may involve patents. The issuing agency of this document is not responsible for identifying these patents.
This standard was proposed by the Institute of Oceanology, Chinese Academy of Sciences.
This standard is under the jurisdiction of the National Marine Standardization Technical Committee (SAC/TC283).
This standard was drafted. Institute of Oceanology, Chinese Academy of Sciences.
The main drafters of this standard. Lei Yanli, Xu Kuidong.
Introduction
This standard was formulated to fill the lack of relevant micro-organisms in GB/T 12763.6 "Specifications for Marine Survey Part 6.Marine Biological Survey"
Regulations for the survey of type benthic organisms.
In order to improve the content of marine surveys in my country, provide accurate, advanced and practical research methods for marine microbenthic surveys, and realize
For the system integration research of large, small and micro benthic organisms, the "908 Special Office" of the State Oceanic Administration organized the formulation of this standard.
This standard is applicable to the survey of marine microbenthos in different habitats such as intertidal zone, shallow sea and deep sea.
The unification of investigation procedures, operational requirements and data processing methods plays an important role in the smooth development of the investigation.
Marine microbenthic survey specifications
1 Scope
This standard determines the basic requirements for marine microbenthic surveys, and establishes sampling and fixation, separation and cultivation, centrifugation and extraction, and staining.
General regulations and methods of color and film production, qualitative and quantitative analysis.
This standard is applicable to the survey of marine microbenthos in different habitats such as intertidal zone, shallow sea and deep sea.
2 Normative references
The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB/T 12763.1 Marine Survey Specification Part 1.General
GB/T 12763.6 Specification for Marine Survey Part 6.Marine Biological Survey
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Microbenthos
All single-celled prokaryotic and eukaryotic micro-organisms that live on the surface and bottom of sediments and are retained by 0.22μm filters.
Note 1.The microbenthic organisms mainly include benthic bacteria (benthicbacteria), cyanobacteria (cyanobacteria), benthic microalgae (benthicmicroalgae) and
Benthic protozoa (benthicprotozoa). Several major taxa are shown in Figure A.1.
Note 2.From the perspective of individual size, microbenthic organisms cover pico-level (pico) with a particle size of less than 2 μm, nano-level (micro) with a particle size of 2 μm to 20 μm, and
20μm micro-level (microminiature) benthic unicellular organisms.
3.2
Benthicprotozoa
Animal unicellular eukaryotes that are associated with sediment or the matrix of water bodies all or most of their life history.
Note. Mainly include heterotrophicflagelate (heterotrophicflagelate), ciliate (ciliate) and sarcodina (sarcodina).
4 Basic requirements
4.1 Main observations
Determine the composition, individual abundance and biomass of main microbenthic groups.
4.2 Auxiliary observation
Community structure, such as species diversity, species richness, evenness index, community similarity, etc., can be based on the assignment or contract, survey plan
Planning, GB/T 12763.6 self-developed.
4.3 Observation site and hierarchical design
4.3.1 Observation sites include marine sampling (core sampling or diving sampling) and intertidal sampling.
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of HYT140-2011_English be delivered?Answer: Upon your order, we will start to translate HYT140-2011_English as soon as possible, and keep you informed of the progress. The lead time is typically 4 ~ 6 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of HYT140-2011_English with my colleagues?Answer: Yes. The purchased PDF of HYT140-2011_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.
|