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HJ 628-2011: Regulation for the collection or genetic resources
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Regulation for the collection or genetic resources
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HJ 628-2011
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Basic data | Standard ID | HJ 628-2011 (HJ628-2011) | | Description (Translated English) | Regulation for the collection or genetic resources | | Sector / Industry | Environmental Protection Industry Standard | | Classification of Chinese Standard | Z01 | | Classification of International Standard | 13.020 | | Word Count Estimation | 8,828 | | Date of Issue | 2011-09-09 | | Date of Implementation | 2012-01-01 | | Quoted Standard | National key protected wild animals, the letter [ 1988 ] No. 144; "national key protected wild plants (the first batch), " the letter [ 1999 ] No. 92 | | Regulation (derived from) | Department of Environmental Protection Notice No. 67 of 2011 | | Issuing agency(ies) | Ministry of Ecology and Environment | | Summary | This standard specifies the wildlife genetic resources collection procedures, technical regulations and precautions. This standard applies to the PRC wildlife genetic resources (animals, plants, fungi and other large) collection. | HJ 628-2011: Regulation for the collection or genetic resources---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Regulation for the collection or genetic resources
People's Republic of China National Environmental Protection Standards
Genetic resources collection technical specification (Trial)
Regulation for the collection of genetic resources
Issued on.2011-09-09
2012-01-01 implementation
Issued by the Ministry of Environmental Protection
Table of Contents
Preface .II
1. Scope ..1
2 Normative references ..1
3 Terms and definitions
4 Basic Principles ..1
5 procedures ..2
Prepare collected before 6 .2
7 Acquisition officers ..2
8 collection of tools and materials ..2
9 ..2 collection methods and objects
Number 10 collected 3
Handling and storage of samples collected 11 .. 3
After 12 animals are treated 4
13 ethics ..4
14 security measures ..4
15 4 record
16 Repeat acquisition ..4
Foreword
To implement the "People's Republic of China Environmental Protection Law", "People's Republic of China Wild Animal Protection Law", "medium
Wild Plant Protection Regulations of the PRC "," Nature Reserve Regulations "People's Republic of China, etc. Regulate Health
Collecting genetic resources, to strengthen the protection of genetic resources, promotion of research and the use of genetic resources and formulate
This standard.
This standard specifies the procedures, technical specifications and precautions wildlife genetic resources within our collection.
This standard is the first release.
This standard is developed by the Ministry of Environmental Protection Science, Technology organization.
This standard is mainly drafted by. Environmental Protection Department of the Nanjing Institute of Environmental Sciences.
This standard MEP September 9, 2011 for approval.
This standard since January 1, 2012 implementation.
The standard explanation by the Ministry of Environmental Protection.
Genetic resources collection Technical Specification
1 Scope
This standard specifies the procedures, technical specifications and precautions wildlife genetic resources collected.
This standard applies to the territory of People's Republic of China wildlife genetic resources (animals, plants, fungi, large, etc.)
collection.
2 Normative references
This standard references the following documents or the terms.
"National key protected wild animal list" (the letter [1988] No. 144)
"National key protected wild plants (the first batch)" (the letter [1999] No. 92)
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1 genetic resources genetic resources
Actual or potential value (including the value of aspects of economic, social, cultural, environmental, etc.), from plants, animals
Any material containing functional units of heredity was, microbial or other origin, including the following species and species taxon
(Subspecies, varieties, variants, species, strain, type), including individual, organ, tissue, cell, chromosome, of DNA
A variety of forms and fragments of genes.
3.2 Non-invasive sampling non-invasive sampling
Without even see the capture of animals, the loss by collecting hair, feathers, feces, food residue,
Sampling methods egg shell, the pupal case and other samples.
3.3 Non-destructive sampling non-destructive sampling
By capturing the animals to collect blood, hair, feathers, scales, toes and other organizations, it does not produce severe physiological function
Sampling methods big impact.
3.4 destructive sampling destructive sampling
Even kill animals to obtain sampling fresh muscles, organs and other tissue samples by serious injury.
4 Basic Principles
4.1 The principle of legality
Collecting genetic resources shall not violate the provisions of relevant state laws and regulations, shall be collected before the relevant provisions of the State
Relevant authorities or local application, to be implemented after the approval of the acquisition.
4.2 Principle of protection
Collecting a biological genetic resources should first select a non-invasive sampling, followed by non-destructive sampling, unless special needs
Shall not carry out destructive sampling. It should control the amount of sample collection to minimize the impact and damage to the organism. Can not because
Collection activities destroy habitats and species populations.
4.3 disclosure of origin principle
Genetic resources collected must disclose its exact origin, including the collection of time, place, and other taxonomic status letter
interest.
5 procedures
Acquisition of basic biological genetic resources program comprising. gathering before preparation, implementation of collection, processing and storage of samples,
Records.
Prepare 6 collected before
6.1 Acquisition national key protected wild animals, wild plant genetic resources (see "national key protected wild animal name
Record "and" national key protected wild plants (first) "), shall be in accordance with the provisions of relevant laws and regulations to phase
Off the competent authorities to apply, to be implemented after the approval of the acquisition. Need to collect genetic resources in nature reserves, shall
Protected area management agencies to apply, to be implemented after the approval of the acquisition.
We should fully study its morphology, physiology and distribution of 6.2 before the acquisition of genetic resources and protection of endangered species, to avoid
Collection activities cause irreparable damage.
6.3 Acquisition should be developed before a complete acquisition program, understand the distribution of regional wildlife, according to the local natural conditions made
Given collection roadmap avoid collection activities aimless destruction of natural habitats.
6.4 collection route should be from the edge of the distribution of biological populations (populations) to the heart of the way forward, the priority acquisition
Population (population) individual marginal distribution. In protected areas such as collection, shall order the experimental zone, buffer zone travel.
7 Acquisition officers
Respond collection personnel specialized training, to master the collection target species taxonomy, morphology and knowledge students
Living habits, habitat and capture skills.
8 collection of tools and materials
8.1 capture tools include. capturing network, homemade traps and tranquilizer gun (needle), etc., should be selected according to the collected objects.
8.2 processing equipment include. scissors (medical or scientific level), scalpel, tweezers, capped plastic tubes (or centrifugation
Tube), capped jars, dropper, sealed plastic bags, tin foil cartridge, latex gloves, hand centrifuge. Equipment in advance
Washed and disinfected to avoid contamination of tissue and infectious diseases.
8.3 recording tools include. marker, label paper, pencils and the like.
8.4 Materials handling and storage comprising. distilled water, liquid nitrogen, dry ice, 90% to 100% ethanol, buffer solution of DNA, anticoagulant
Wait.
9 collection methods and objects
9.1 Basic requirements
First select the collection of genetic resources should be non-invasive sampling, by collecting animal loose hair or feathers, manure
Stools, food scraps, egg shells, pupa shells and other samples, genetic resources extracted from the sample remaining cells. In a non-loss can not be
When traumatic samples or genetic resources need higher quality, it can be non-destructive sampling. Unless special needs can not be hurt
Victims of sampling. Common non-destructive sampling mode for each taxa see 9.2-9.7.
9.2 mammal
Available shot capture large mammals anesthesia; small mammals such as rodents, etc. can catch live capture cage, then
Anesthetic anesthesia. After the catch mammals collected from hair or neck (ear) venous blood with hair follicles. Collection object
Mature individuals based, try not to catch larvae and breeding, lactating mother.
9.3 Birds
Bird netting is available, according to the mesh size of the birds body changes. Birds in their choice based on the habits of infested forest edge, water
Fields, meadows and other places set up network. Birds caught after collecting feathers or wings (legs) venous blood.
9.4 Amphibians and Reptiles
Amphibians and reptiles can be traps, nets, and other methods lasso capture, after capture clipping their toes or tail tip.
9.5 Fish
Fish using fishing nets, gear selection and mesh size according to the size of fish to be. After peeling scales or capture clip
Rays.
9.6 invertebrates
Should be developed in accordance with the acquisition method invertebrate taxa and their living habits. For flying insects can sweep netting, lights
Lure capture; crawling insects and mollusks and other traps can capture; aquatic and benthic mollusks, and Platyhelminthes
And other arthropods can dip net, trawling, dredging and other fishing tools. After obtaining invertebrate collection wings, shells, muscle
Meat and other organizations.
9.7 Large plants and fungi
Large collection of plants and fungi should refer to the principle of non-destructive sampling of the animal can be collected fresh leaves, buds, flowers,
Seeds, fruiting bodies and other organizations, try not to collect underground roots, stems.
Number 10 collected
We should try to collect a small amount of sample, usually 50 μl of blood or tissue to 200 mg. If the research or development work required
To be appropriate to increase the collection amount, but not for the normal growth of the organism, affecting reproduction and activities. Should be strictly controlled weight
Collection points and the amount of protection of endangered species.
Handling and storage of samples collected 11
11.1 Acquisition should wear sterile latex gloves, use forceps samples taken after sample collection with distilled water.
11.2 animal tissue into a clean plastic tube or jar, sealed and stored in liquid nitrogen or dry ice can also be dropped
90% to 100% ethanol or DNA buffer soaking the tissue preservation.
11.3 blood sample should be thoroughly mixed with anticoagulants, then wait for it to naturally settle or centrifuge from the use of the hand
Heart, blood cells and plasma separation, discard the plasma, blood cells stored in liquid nitrogen or dry ice.
11.4 plant tissue in a plastic bag, 1.10 silica gel was added, followed by extrusion which air bag after closure
Put tinfoil box stored.
11.5 samples taken at least two points to be stored in order to avoid risks. Samples should be sent to a laboratory experiment or within 1 week
Long-term preservation.
12 animals are treated
For the complete collection of animal genetic resources can not be abandoned, it should disinfect the wound and stopped the bleeding, wait for it to complete
After waking up in situ release. Animals must not be taken outside the original habitat release.
13 Code of Ethics
When live animals are processed, the operation should be fast, be careful, try to alleviate their suffering.
14 security measures
To make full safety precautions when collecting wild animal genetic resources. Capture and processing of wild beasts, snakes and other dangerous
Note that when the risk of animal safety, avoid for its injuries. For potential foci of animals such as rodents, birds, etc., should pay attention to anti
Phytophthora protection, immunization injections if necessary.
15 Records
15.1 samples of genetic resources collected to timely numbered tag and do detailed records in order to provide the relevant competent ministries
Doors and protected areas for the record.
Basic Contents 15.2 records shall include at least.
(1) Acquisition of basic human information (name, ID number, affiliation, address, contact information);
(2) approval authorities and approval document number;
(3) The purpose of the acquisition (the stated purpose of the use of genetic resources collection);
(4) Acquisition time;
(5) sample number;
(6) Location information collection (location, type of vegetation, habitat status, latitude and longitude coordinates, altitude, temperature
, Humidity, photographs, etc.);
(7) Basic information Genetic Resources (Chinese name, scientific name, taxonomic status, collecting forms, collecting quantity) acquisition.
16 Repeat collection
In general, the acquisition must be submitted again to repeat acquisition. But for the purposes of scientific research universities and research centers
Which may be mutually agreed and relevant departments signed the book a certain period of acquisition plans.
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