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HJ 505-2009 English PDF

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HJ 505-2009English369 Add to Cart 3 days [Need to translate] Water quality. Determination of biochemical oxygen demand after 5 days(BOD5) for dilution and seeding method Valid HJ 505-2009


BASIC DATA
Standard ID HJ 505-2009 (HJ505-2009)
Description (Translated English) Water quality. Determination of biochemical oxygen demand after 5 days(BOD5) for dilution and seeding method
Sector / Industry Environmental Protection Industry Standard
Classification of Chinese Standard Z16
Classification of International Standard 13.060
Word Count Estimation 14,147
Date of Issue 2009-10-20
Date of Implementation 2009-12-01
Older Standard (superseded by this standard) GB/T 7488-1987
Quoted Standard GB/T 6682; GB/T 7489; GB/T 11913; HJ/T 91
Adopted Standard ISO 5815-1-2003, MOD; ISO 5815-2-2003, MOD
Drafting Organization Shenyang Municipal Environmental Monitoring Center Station
Administrative Organization Ministry of Environment Protection
Regulation (derived from) Department of Environmental Protection Notice No. 54 of 2009
Summary This standard specifies the determination of BOD (BOD (5)) Dilution and seeding method. This standard applies to surface water, industrial wastewater and domestic sewage BOD (BOD (5)) determination. The detection limit of 0. 5mg/L, the method detection limit of 2mg/L, non- dilution and non- dilution inoculation method determination limit of 6mg/L, diluted with dilution inoculation method determination limit of 6000mg/L.

Standards related to: HJ 505-2009

HJ 505-2009
Water quality.Determination of biochemical oxygen demand after 5 days(BOD5) for dilution and seeding method
HJ
National Environmental Protection Standard of the People's Republic
Replace GB/T 7488-1987
Determination of biochemical oxygen demand (BOD5) on the fifth day of water quality
Dilution and inoculation
Water quality-Determination of biochemical oxygen demand after 5 days
(BOD5)for dilution and seeding method
Released on.2009-10-20
2009-12-01 Implementation
Ministry of Environmental Protection released
Ministry of Environmental Protection
announcement
No. 54 of.2009
In order to implement the "Environmental Protection Law of the People's Republic of China", protect the environment, and protect human health, we now approve the measurement of total organic carbon in water quality.
Six standards, such as the combustion oxidation-non-dispersive infrared absorption method, are national environmental protection standards and are released.
The standard name and number are as follows.
I. Determination of total organic carbon in water by combustion oxidation-non-dispersive infrared absorption method (HJ 501-2009);
2. Determination of brominated volume of volatile phenols in water (HJ 502-2009);
3. Determination of volatile phenols in water - 4-aminoantipyrine spectrophotometric method (HJ 503-2009);
4. Determination of ozone in ambient air - Spectrophotometric method of sodium indigo disulphonate (HJ 504-2009);
6. "Electrochemical Probe Method for Determination of Dissolved Oxygen in Water" (HJ 506-2009).
The above standards have been implemented since December 1,.2009 and published by the China Environmental Science Press. The standard content can be found on the website of the Ministry of Environmental Protection.
The following seven national rings approved and issued by the former National Environmental Protection Agency or the former State Environmental Protection Administration from the date of implementation of the above standards
The environmental protection standards are abolished. The standard names and numbers are as follows.
1. "Measurement of total organic carbon (TOC) for water quality by non-dispersive infrared absorption method" (GB 13193-91);
2. Determination of total organic carbon in water by combustion oxidation-non-dispersive infrared absorption method (HJ/T 71-2001);
3. Determination of volatile phenols in water quality and bromination capacity after distillation (GB 7491-87);
4. Determination of volatile phenols in water - 4-aminoantipyrine spectrophotometric method after distillation (GB 7490-87);
V. Determination of ozone in ambient air - Spectrophotometric method of sodium indigo disulphonate (GB/T 15437-1995);
6. "Determination of the Biochemical Oxygen Demand (BOD5) on the 5th Day of Water Quality Dilution and Inoculation Method" (GB 7488-87);
VII. Determination of Dissolved Oxygen in Water by Electrochemical Probe Method (GB 11913-89).
Special announcement.
October 20,.2009
Content
Foreword..iv
1 Scope..1
2 Normative references..1
3 method principle..1
4 Reagents and materials.1
5 instruments and equipment. 2
6 samples.3
7 Analysis steps..3
8 result calculation..6
9 Quality Assurance and Quality Control 6
10 Precision and Accuracy 7
Appendix A (informative appendix) This standard chapter number is compared with ISO 5815-1, 2.2003 chapter number 8
Foreword
To protect the environment and protect the human body in order to implement the Environmental Protection Law of the People's Republic of China and the Law of the People's Republic of China on Water Pollution Prevention and Control
This standard is established for the determination of the five-day biochemical oxygen demand (BOD5) in the water.
This standard specifies the method for determining the dilution and inoculation of BOD5 on surface water, industrial wastewater and domestic sewage.
This standard is a revision of the "Dilution and Inoculation Method for the Determination of Biochemical Oxygen Demand (BOD5) on the 5th Day of Water Quality" (GB 7488-87).
This standard is modified to use ISO 5815-1.2003 "Determination of water quality n day biochemical oxygen demand (BODn). Part 1. Adding propylene
Determination of the thiourea dilution and inoculation method and ISO 5815-2.2003 "Water quality n day biochemical oxygen demand (BODn) determination Part II.
Non-dilution method (English version). For the convenience of comparison, the chapter number of this standard and ISO 5815-1, 2 are listed in the informative Appendix A.
A comparison checklist of the.2003 chapter number is for reference.
This standard was first published in 1987. The original standard was drafted by Beijing Institute of Civil Engineering and Architecture. This is the first revision.
The main contents of this revision are as follows.
-- Increased detection limits;
- The principle of the method clearly specifies the culture temperature and time, and increases the content of (25) days of cultivation;
- increased the choice of inoculum;
-- Increased sample pretreatment method content;
- Increased the content of the method for determining the dilution factor of the dilution inoculation method;
-- Refined the method for determining the biochemical oxygen demand on the 5th day;
-- Added quality assurance and quality control chapters.
The national environmental protection standard “Water quality approved and issued by the former National Environmental Protection Agency on March 14, 1987 since the implementation of this standard.
The 5th day biochemical oxygen demand (BOD5) determination of dilution and inoculation method (GB 7488-87) was abolished.
Appendix A of this standard is an informative annex.
This standard was formulated by the Science and Technology Standards Department of the Ministry of Environmental Protection.
This standard is mainly drafted by. Shenyang Environmental Monitoring Center Station.
This standard was approved by the Ministry of Environmental Protection on October 20,.2009.
This standard has been implemented since December 1,.2009.
This standard is explained by the Ministry of Environmental Protection.
Iv
Water quality five-day biochemical oxygen demand (BOD5) determination dilution and inoculation method
Warning. Propylene thiourea is a toxic compound. Wear protective equipment when handling. Avoid contact with skin.
And the preparation of the standard solution should be carried out in a fume hood; the residual residue after the test should be properly and safely disposed.
1 Scope of application
This standard specifies the method for determining the dilution and inoculation of BOD5 in water for five days.
This standard applies to the determination of BOD5 in surface water, industrial wastewater and domestic sewage.
The detection limit of the method is 0.5 mg/L, the lower limit of determination of the method is 2 mg/L, and the upper limit of determination by non-dilution method and non-dilution method is 6
The upper limit of the mg/L, dilution and dilution inoculation method is 6 000 mg/L.
2 Normative references
The contents of this standard refer to the terms in the following documents. For undated references, the valid version applies to this standard.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 7489 Determination of dissolved oxygen in water - Iodometric method
GB/T 11913 Determination of dissolved oxygen in water - Electrochemical probe method
HJ/T 91 Surface Water and Wastewater Monitoring Technical Specifications
3 Principle of the method
Biochemical oxygen demand refers to the decomposition of certain oxidizable substances in water, especially organisms that decompose organic matter, under specified conditions.
Dissolved oxygen consumed by the chemical process. Usually, the water sample is filled in a completely closed dissolved oxygen bottle and cultured in the dark at (20 ± 1) °C.
5d ± 4 h or (2 5) d ± 4 h [first cultured in the dark at 0 ~ 4 ° C for 2 d, then cultured in the dark at (20 ± 1) ° C for 5 d, ie culture (2 5)
d], respectively, determine the mass concentration of dissolved oxygen in the water sample before and after the culture, and calculate the difference in mass concentration of dissolved oxygen before and after the culture.
The amount of dissolved oxygen consumed is expressed in the form of BOD5.
If the content of organic matter in the sample is large, the mass concentration of BOD5 is greater than 6 mg/L, and the sample needs to be properly diluted and determined;
Industrial wastewater with low microorganisms, such as acidic wastewater, alkaline wastewater, high-temperature wastewater, cryopreserved wastewater, or wastewater treated with chlorination,
Inoculation should be carried out in the determination of BOD5 to introduce microorganisms capable of decomposing organic matter in wastewater. When it is difficult to be used by ordinary domestic sewage
When the microorganisms in the microorganisms degrade at a normal rate or contain highly toxic substances, the domesticated microorganisms should be introduced into the water samples for inoculation.
4 reagents and materials
The reagents used in this standard are analyzed using analytical analytical reagents that meet national standards unless otherwise stated.
4.1 Water. The experimental water is Grade 3 distilled water in accordance with GB/T 6682, and the mass concentration of copper ions in the water is not more than 0.01 mg/L.
Does not contain substances such as chlorine or chloramine.
4.2 Inoculum. Inoculum for inoculation of microorganisms can be purchased. The preparation and use of the inoculum should be carried out in accordance with the instructions. Can also press
The next method is to obtain an inoculum.
4.2.1 Domestic sewage not contaminated by industrial wastewater. chemical oxygen demand is not more than 300 mg/L, total organic carbon is not more than 100 mg/L.
4.2.2 River water or lake water containing urban sewage.
4.2.3 The effluent from the sewage treatment plant.
4.2.4 When analyzing industrial wastewater containing refractory substances, take a water sample at the appropriate place downstream of the sewage outlet as a domesticated inoculum for wastewater. and also
It is advisable to neutralize or properly dilute the wastewater for continuous aeration, add a small amount of this kind of wastewater every day, and add a small amount of domestic sewage to make it suitable.
Microorganisms that should be planted with water are multiplied. When a large amount of floc appears in the water, it indicates that the microorganism has propagated and can be used as an inoculum. general
The domestication process takes 3-8 days.
4.3 salt solution
4.3.1 Phosphate buffer solution. 8.5 g potassium dihydrogen phosphate (KH2PO4), 21.8 g dipotassium hydrogen phosphate (K2HPO4), 33.4 g heptahydrate
Disodium hydrogen phosphate (Na2HPO4·7H2O) and 1.7 g ammonium chloride (NH4Cl) are dissolved in water and diluted to 1 000 ml. This solution is in 0~4
°C can be stored for 6 months. This solution has a pH of 7.2.
4.3.2 Magnesium Sulfate Solution, ρ (MgSO4) = 11.0 g/L. Dissolve 22.5 g of magnesium sulfate heptahydrate (MgSO4·7H2O) in water and dilute
To 1 000 ml, this solution can be stored stably for 0 months at 0 to 4 ° C. If any precipitation or microbial growth is found, it should be discarded.
4.3.3 Calcium chloride solution, ρ (CaCl2) = 27.6 g/L. Dissolve 27.6 g of anhydrous calcium chloride (CaCl2) in water and dilute to 1 000 ml.
This solution can be stored stably for 0 months at 0 to 4 ° C. If any precipitation or microbial growth is found, it should be discarded.
4.3.4 ferric chloride solution, ρ (FeCl3) = 0.15 g/L. dissolve 0.25 g of ferric chloride hexahydrate (FeCl3 · 6H2O) in water, dilute to
1 000 ml, this solution can be stored stably for 0 months at 0~4 °C. If any sediment or microbial growth is found, it should be discarded.
4.4 Dilution water. Add a certain amount of water to a glass bottle of 5 to 20 L, control the water temperature at (20 ± 1) °C, and use an aeration device (5.9) to
Less aeration for 1 h, so that the dissolved oxygen in the dilution water reaches 8 mg/L or more. Add the above four salt solutions (4.3) per liter of water before use.
Ml, mix and store at 20 °C. Prevent contamination during aeration, especially to prevent the introduction of organic matter, metals, oxides or reducing substances.
The mass concentration of oxygen in the diluted water should not be supersaturated. It should be placed for 1 h before use and should be used within 24 h. The remaining dilution water should
Discard.
4.5 Inoculation of dilution water. According to the source of the inoculum, add appropriate amount of inoculum per liter of dilution water (4.4) (4.2). urban pollution
Water and sewage treatment plant effluent plus 1 ~ 10 ml, river or lake water plus 10 ~ 100 ml, inoculated dilution water stored in (20 ± 1) ° C environment
In the day, it will be used on the same day. The pH of the inoculated dilution water is 7.2 and the BOD5 should be less than 1.5 mg/L.
4.6 Hydrochloric acid solution, c (HCl) = 0.5 mol/L. Dissolve 40 ml of concentrated hydrochloric acid (HCl) in water and dilute to 1 000 ml.
4.7 Sodium hydroxide solution, c (NaOH) = 0.5 mol/L. Dissolve 20 g of sodium hydroxide in water and dilute to 1 000 ml.
4.8 Sodium sulfite solution, c(Na2SO3)=0.025 mol/L. Dissolve 1.575 g of sodium sulfite (Na2SO3) in water and dilute to 1 000 ml.
This solution is unstable and needs to be used now.
4.9 Glucose-glutamic acid standard solution. glucose (C6H12O6, pure grade) and glutamic acid (HOOC-CH2-CH2-CHNH2-COOH,
It is dried at 130 ° C for 1 h, and each 150 mg is dissolved in water and diluted to the mark in a 1 000 ml volumetric flask. BOD5 of this solution
It is (210±20) mg/L, and it is used now. The solution can also be cryopreserved in small amounts and used immediately after thawing.
4.10 Propylene thiourea nitrate inhibitor, ρ (C4H8N2S) = 1.0 g/L. dissolve 0.20 g of propylene thiourea (C4H8N2S) in.200 ml water
Mix in medium, store at 4 ° C, this solution can be stored for 14 days.
4.11 Acetic acid solution, 1 1.
4.12 Potassium iodide solution, ρ (KI) = 100 g/L. Dissolve 10 g of potassium iodide (KI) in water and dilute to 100 ml.
4.13 Starch solution, ρ = 5 g/L. Dissolve 0.50 g of starch in water and dilute to 100 ml.
5 Instruments and equipment
Unless otherwise stated, this standard uses glass gauges that meet national Class A standards. The glass instruments used in this standard shall be clear.
A clean, non-toxic and biodegradable substance.
5.1 Filter. The pore size is 1.6 μm.
5.2 Dissolved oxygen bottle. with water sealing device, the volume is 250 ~ 300 ml.
5.3 Dilution container. 1 000 to 2 000 ml graduated cylinder or volumetric flask.
5.4 Siphon. For water sampling or adding dilution water.
5.5 Dissolved Oxygen Analyzer.
5.6 Refrigerator. 0 ~ 4 °C.
5.7 Refrigerator. Has the function of freezing and refrigerating.
5.8 Constant temperature incubator with fan. (20 ± 1) °C.
5.9 Aeration device. multi-channel air pump or other aeration device; aeration may bring organic matter, oxidant and metal, resulting in air pollution,
If there is pollution, the air should be filtered and cleaned.
6 samples
6.1 Acquisition and preservation
Sample collection is performed in accordance with the relevant regulations of HJ/T 91.
The collected sample should be filled and sealed in a brown glass bottle with a sample volume of not less than 1 000 ml and transported and stored in a dark place at 0 to 4 °C.
And analyze it as soon as possible within 24 hours. Cannot be analyzed within 24 h, can be stored frozen (avoid sample bottle rupture during cryopreservation), before freezing sample analysis
Thaw, homogenize and inoculate.
6.2 Sample pretreatment
6.2.1 pH adjustment
If the sample or diluted sample pH is not in the range of 6 to 8, adjust it with hydrochloric acid solution (4.6) or sodium hydroxide solution (4.7).
The pH is from 6 to 8.
6.2.2 Removal of residual chlorine and combined chlorine
If the sample contains a small amount of residual chlorine, it is usually placed for 1 to 2 hours after sampling, and the free chlorine disappears. For those that cannot disappear in a short time
Chlorine, an appropriate amount of sodium sulfite solution can be added to remove the residual chlorine and combined chlorine present in the sample. The amount of sodium sulfite solution added is determined by the following method.
set.
Take 100 ml of the neutralized water sample, add 10 ml of acetic acid solution (4.11), 1 ml of potassium iodide solution (4.12), mix, and immerse in the dark.
Set for 5 min. The precipitated iodine was titrated to a pale yellow color with a sodium sulfite solution, and a 1 ml starch solution (4.13) was added in a blue color. Continue to titrate until
Blue has just faded, that is, the end point, record the volume of sodium sulfite solution used, the volume consumed by the sodium sulfite solution, calculate the water sample
The volume of sodium sulfite solution.
6.2.3 Sample homogenization
Samples containing large amounts of particulate matter, requiring large dilutions or cryopreserved samples should be stirred evenly before measurement.
6.2.4 Algae in the sample
If a large amount of algae is present in the sample, the determination of BOD5 will be high. When the accuracy of the analysis results is high, the filter is applied before the measurement.
The wells were filtered through a 1.6 μm filter (5.1) and the size of the filter pores was noted in the test report.
6.2.5 Samples with low salt content
If the salt content of the sample is low and the conductivity of the undiluted sample is less than 125 μS/cm, add an appropriate amount of the same volume of the four salt solutions (4.3).
The conductivity of the sample was made greater than 125 μS/cm. The volume V of each salt to be added per liter of sample is calculated according to formula (1).
( 12.8)/113V K .6= Δ − (1)
Where. V--the volume of each salt to be added, ml;
ΔK--The sample requires an increased conductivity value, μS/cm.
7 Analysis steps
7.1 Non-dilution method
The non-dilution method is divided into two cases. non-dilution method and non-dilution method.
If the content of organic matter in the sample is small, the mass concentration of BOD5 is not more than 6 mg/L, and there are enough microorganisms in the sample.
Method of determination. If the amount of organic matter in the sample is small, the mass concentration of BOD5 is not more than 6 mg/L, but there is not enough microorganism in the sample.
For example, acidic wastewater, alkaline wastewater, high-temperature wastewater, cryopreserved wastewater, or chlorinated wastewater are measured by non-dilution inoculation.
7.1.1 Preparation of samples
7.1.1.1 Samples to be tested
Before the measurement, the temperature of the sample to be tested reaches (20±2) °C. If the dissolved oxygen concentration in the sample is low, it needs to be aerated with aeration device (5.9).
15 min, shake thoroughly to remove the air bubbles remaining in the sample; if the oxygen in the sample is supersaturated, fill the container with 2/3 volume and shake vigorously
The saturated oxygen is driven out, and the determination method is determined according to the microbial content in the sample. Non-dilution method can be directly sampled; non-dilution vaccination
Method, add appropriate amount of inoculum (4.2) per liter of sample, to be determined. If the sample contains nitrifying bacteria, nitrification may occur.
2 ml of propylene thiourea nitrate inhibitor (4.10) was added to each liter of sample.
7.1.1.2 Blank specimen
In the non-dilution inoculation method, add the same amount of inoculum (4.2) as the blank sample per liter of diluted water, and test each liter if necessary.
2 ml of propylene thiourea nitrate inhibitor (4.10) was added to the sample.
7.1.2 Determination of samples
7.1.2.1 Determination of dissolved oxygen in samples by iodometric method
Fill the sample (7.1.1.1) with two dissolved oxygen bottles (5.2) to make the sample overflow a small amount to prevent the dissolved oxygen concentration in the sample from changing.
Change, so that the bubbles present in the bottle are discharged through the wall of the bottle. Put a bottle cap on the bottle, add a water seal, and put a sealing cover on the cap cover to prevent culture.
During the period, the water-sealed water is evaporated and dried. After incubating for 5d ± 4 h or (2 5) d ± 4 h in a constant temperature incubator (5.8), the dissolved oxygen in the sample is determined to be rich.
degree. The mass concentration of dissolved oxygen in the sample before the culture was measured 15 minutes after the other bottle.
The dissolved oxygen was measured in accordance with GB/T 7489.
7.1.2.2 Determination of dissolved oxygen in samples by electrochemical probe method
Fill the sample (7.1.1.1) with a dissolved oxygen bottle (5.2) to make the sample overflow a small amount to prevent the dissolved oxygen concentration in the sample from changing.
Change, so that the bubbles present in the bottle are discharged through the wall of the bottle. The mass concentration of dissolved oxygen in the sample before the culture was measured.
Cover the cap to prevent residual air bubbles in the sample, plus a water seal, and a sealing cover on the cap cover to prevent the water seal water from evaporating during the culture.
dry. Place the vial in a constant temperature incubator (5.8) for 5 d ± 4 h or (2 5) d ± 4 h. Determine the quality of dissolved oxygen in the sample after culture
concentration.
The determination of dissolved oxygen is carried out in accordance with GB/T 11913.
Blank samples are measured in the same way as 7.1.2.1 or 7.1.2.2.
7.2 Dilution and inoculation
The dilution and inoculation methods are divided into two cases. dilution method and dilution inoculation method.
If the content of organic matter in the sample is large, the mass concentration of BOD5 is greater than 6 mg/L, and there are enough microorganisms in the sample to be diluted.
Method determination; if the content of organic matter in the sample is large, the mass concentration of BOD5 is greater than 6 mg/L, but there is not enough microorganism in the sample,
Determined by dilution inoculation method.
7.2.1 Preparation of samples
7.2.1.1 Samples to be tested
The temperature of the sample to be tested reaches (20±2) °C. If the dissolved oxygen concentration in the sample is low, it needs to be aerated for 15 min with aeration device (5.9).
Shake off the residual air bubbles in the sample; if the oxygen in the sample is supersaturated, fill the 2/3 volume of the container with the sample and force it to shake out the fullness.
And oxygen, and then determine the method based on the microbial content of the sample. Determined by the dilution method, the dilution factor is determined according to the methods in Table 1 and Table 2,
It was then diluted with dilution water (4.4). The sample was diluted by inoculating dilution water (4.5) as determined by dilution inoculation. If the sample contains nitrifying bacteria,
Nitrification may occur, and 2 ml of propylene thiourea nitrate inhibitor (4.10) should be added to each liter of the culture solution.
Determination of dilution factor. The degree of dilution of the sample should be such that the dissolved dissolved oxygen concentration is not less than 2 mg/L, and remains in the sample after culture.
The dissolved oxygen mass concentration is not less than 2 mg/L, and the mass concentration of dissolved oxygen remaining in the sample is preferably 1/3 to 2/3 of the starting concentration.
The dilution factor can be determined from the total organic carbon (TOC), permanganate index (IMn) or chemical oxygen demand (CODCr) of the sample.
Estimate the ratio of BOD5 to total organic carbon (TOC), permanganate index (IMn) or chemical oxygen demand (CODCr) according to Table 1.
The expected value of BOD5 (R is related to the type of sample), and then the dilution factor is determined according to Table 2. When the dilution factor cannot be accurately selected,
One sample is made in 2 to 3 different dilution factors.
Table 1 Typical ratio R
Type of water sample total organic carbon R (BOD5/T OC)
Permanganate index R
(BOD5/IMn)
Chemical oxygen demand R
(BOD5/CODCr)
Untreated wastewater 1.2~2.8 1.2~1.5 0.35~0.65
Biochemical treatment of wastewater 0.3~1.0 0.5~1.2 0.20~0.35
Calculate the expected value of BOD5 according to equation (2) by selecting the appropriate R value in Table 1.
R Yρ = ⋅ (2)
Where. ρ -- the expected value of the five-day biochemical oxygen demand concentration, mg/L;
Y--Total organic carbon (TOC), permangana......
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