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HJ 442.6-2020 English PDF

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HJ 442.6-2020: (Technical specifications for environmental monitoring in coastal waters - Part 6: Biological monitoring in coastal waters)
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HJ 442.6-2020English219 Add to Cart 3 days [Need to translate] (Technical specifications for environmental monitoring in coastal waters - Part 6: Biological monitoring in coastal waters) Valid HJ 442.6-2020

PDF similar to HJ 442.6-2020


Standard similar to HJ 442.6-2020

GB 8702   HJ 630   HJ 607   HJ 442.1   HJ 442.2   HJ 442.10   

Basic data

Standard ID HJ 442.6-2020 (HJ442.6-2020)
Description (Translated English) (Technical specifications for environmental monitoring in coastal waters - Part 6: Biological monitoring in coastal waters)
Sector / Industry Environmental Protection Industry Standard
Classification of Chinese Standard Z06
Classification of International Standard 13.020
Word Count Estimation 10,160
Date of Issue 2020-12-16
Date of Implementation 2021-03-01
Older Standard (superseded by this standard) HJ 442-2008
Regulation (derived from) Ministry of Ecology and Environment Announcement No. 66 [2020]
Issuing agency(ies) Ministry of Ecology and Environment

HJ 442.6-2020: (Technical specifications for environmental monitoring in coastal waters - Part 6: Biological monitoring in coastal waters)


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(Technical specifications for environmental monitoring in coastal waters Part 6 Biological monitoring in coastal waters) National Environmental Protection Standards of the People's Republic of China Replace HJ 442-2008 Technical specifications for environmental monitoring in coastal waters Part VI Biological Monitoring in Inshore Waters Technical specification for offshore environmental monitoring Part 6 offshore biological monitoring 2020-12-16 release 2021-03-01 implementation Issued by the Ministry of Ecology and Environment i table of contents Foreword...Ⅱ 1 Scope of application...1 2 Normative references...1 3 Terms and definitions...1 4 General requirements for biological monitoring in coastal waters...3 5 Collection, storage and transportation of biological samples...3 6 Biological sample analysis...5 7 Quality Control of Biological Monitoring...5

Foreword

To implement the Environmental Protection Law of the People’s Republic of China, the Marine Environmental Protection Law of the People’s Republic of China, and the "Regulations on the Administration of the Prevention and Control of Land-Source Pollutants Pollution and Damage to the Marine Environment" of the People’s Republic of China "Regulations on the Management of Pollution Damage to the Marine Environment" and the "Administrative Measures for Environmental Functional Zones in Coastal Sea Areas" to regulate the ecological environment of coastal sea areas Quality monitoring, protecting the ecological environment, and ensuring the scientificity, accuracy, systematicness and comparability of the national coastal waters environmental monitoring And representativeness, formulate this standard. This standard was first published in.2008.The original standard drafting unit was China Environmental Monitoring Station and Zhoushan Marine Biotechnology Environmental Monitoring Station. This is the first revision. The revised standard consists of the following ten parts. Part One General Provisions Part Two. Data Processing and Information Management Part III Water Quality Monitoring of Inshore Waters Part Four. Monitoring of Sediment in Coastal Seas Part V Monitoring of Biological Quality in Coastal Waters Part VI Biological Monitoring in Inshore Waters Part VII Monitoring of rivers entering the sea Part 8.Monitoring of Direct Discharge of Sea Pollution Sources and Impact on the Water Environment of Inshore Waters Part IX Emergency and Special Monitoring in Coastal Waters Part 10 Evaluation and Report This standard is the sixth part of the revised standard. The revision is mainly aimed at the sampling of samples for biological monitoring in coastal waters in the original standard. The methods and procedures of collection, preservation, transportation, laboratory analysis and quality control are mainly revised in the following aspects. --Regulate the monitoring content in accordance with routine monitoring items and other monitoring items; --Add content to determine the detection limit of chlorophyll a; --The qualitative and quantitative sampling of macrobenthos is used to replace the original on-bottom and in-bottom biological sampling. From the date of implementation of this standard, the "Environmental Monitoring Specifications for Coastal Sea Areas" (HJ 442-2008) shall be abolished. This standard was formulated by the Department of Ecological Environment Monitoring and the Department of Regulations and Standards of the Ministry of Ecology and Environment. Drafting organizations of this standard. China Environmental Monitoring Station, Zhoushan Marine Ecological Environmental Monitoring Station of Zhejiang Province, Tianjin Ecological Environmental Monitoring Center, Dalian Ecological Environmental Monitoring Center of Liaoning Province. This standard was approved by the Ministry of Ecology and Environment on December 16, 2020. This standard will be implemented on March 1, 2021. This standard is interpreted by the Ministry of Ecology and Environment.

1 Technical specifications for environmental monitoring in coastal waters

Part VI Biological Monitoring in Inshore Waters

1 Scope of application

This standard specifies the technical requirements for sample collection, storage, transportation, analysis, and quality control for biological monitoring in coastal waters. This standard applies to biological monitoring in coastal waters.

2 Normative references

The content of this standard refers to the terms in the following documents. For undated references, the effective version is applicable to this standard. GB 17378.7 Marine Monitoring Specification Part 7.Offshore Pollution Ecological Investigation and Biological Monitoring GB/T 17826 Marine life classification code HJ 168 Technical Guidelines for the Preparation and Revision of Environmental Monitoring and Analysis Methods HJ 442.1 The general rules of the first part of the technical specifications for environmental monitoring in coastal waters HJ 442.2 Technical Specification for Environmental Monitoring in Coastal Seas Part 2 Data Processing and Information Management HJ 442.3 Technical Specifications for Environmental Monitoring in Coastal Seas Part 3 Water Quality Monitoring in Coastal Seas HJ 442.10 Technical Specifications for Environmental Monitoring in Coastal Seas Part Ten Evaluation and Report HJ 730 Technical Specification for Layout of Environmental Monitoring Points in Coastal Waters HY/T 147.5 Technical Regulations for Marine Monitoring Part 5.Marine Ecology

3 Terms and definitions

The following terms and definitions apply to this standard. 3.1 Biological monitoring Periodic and fixed-point (regional) analysis and determination of biological individuals, populations or communities caused by environmental pollution or changes The environmental monitoring category that clarifies the environmental pollution situation. 3.2 Phytoplankton It is a kind of autotrophic plankton, mostly single-celled plants, with chlorophyll or other pigment bodies, which can absorb light energy and Carbon dioxide conducts photosynthesis and makes organisms (mainly carbohydrates) by itself. Mainly include diatoms, dinoflagellate, green Algae, cyanobacteria, golden algae, xanthellae and algae spores, etc., they are the main producers of waters. 23.3 Zooplankton zooplankton Small animals that live in the water layer and move passively are collectively called zooplankton. Including planktonic protozoa, coelenterate, Pteropods and heteropods of molluscs, crustaceans, chaetognaths, tunicates, planktonic larvae and other phyla Individual floating types, etc. According to individual size, it can be divided into giant zooplankton, large zooplankton, medium and small zooplankton and miniature zooplankton. Zooplankton. The large zooplankton and medium and small zooplankton in this standard refer to the use of GB 17378.7 shallow water I and II respectively Zooplankton collected by the type plankton net. 3.4 Microorganism Refers to many groups of organisms that are small and generally require the help of a microscope to be recognized. Microorganisms in a broad sense include bacteria, Actinomycetes, molds, yeasts, spirochetes, rickettsia, mycoplasma, chlamydia, viruses, viroids, protozoa And unicellular algae. 3.5 Community structure It is one of the characteristics of the overall level of the biological community, and the most important item in a series of attributes of the community. Community structure package Including nutritional structure, spatial structure (vertical distribution and horizontal distribution), time structure (circadian rhythm and seasonal distribution) and physical Kinds of structure and other aspects. 3.6 Dominant species Refers to the species with a large number and high frequency in an ecosystem or community. 3.7 Species diversity The index is measured by the number of species in a community and their relative abundance, including the number of existing species in the community Order also includes the relative abundance of species (ie, homogeneity), that is, the abundance and heterogeneity of species within a community or ecosystem. 3.8 Evenness An index reflecting the uniformity of the community structure. 3.9 Richness An index indicating the abundance of species in a community (or sample). 3.10 Intertidal benthos 3 Plants living on the bottom surface of the intertidal zone and animals on the bottom surface and in the bottom. 3.11 Macrobenthos The animals and plants that live on, in or near the bottom of waters are collectively called benthic organisms. Macrobenthos A type of organisms, benthic organisms that cannot pass through a 0.5 mm mesh screen.

4 General requirements for biological monitoring in coastal waters

The development of the implementation plan for coastal biological monitoring shall be implemented in accordance with the relevant requirements of HJ 442.1; the layout of monitoring points shall be in accordance with HJ 730 Implementation requirements; data processing and data reporting shall be implemented in accordance with HJ 442.2; evaluation and reporting shall be implemented in accordance with HJ 442.10. The biological monitoring frequency for routine monitoring of important estuaries and bays and key regions determined by the state is based on dry, high, and normal water periods each year. Perform three times, sampling and water quality monitoring are combined; other areas can be carried out according to monitoring conditions and evaluation requirements, such as Monitoring is carried out during high and normal water periods or the same water period is selected for monitoring once every 2 years. Routine monitoring items include phytoplankton, macroplankton, chlorophyll a, fecal coliforms, macrobenthos (fixed the amount). The time and frequency of biological monitoring in other coastal waters are determined according to the monitoring purpose; other monitoring items generally include red tide Animals, small and medium zooplankton, macrobenthos (qualitative), macroalgae, total number of bacteria and fish avoidance response. 5 Collection, storage and transportation of biological samples 5.1 Sampling level a) The sampling level of each monitoring item shall be implemented in accordance with GB 17378.7 and HJ 442.3; b) Microorganisms take surface samples; c) The sampling level of chlorophyll a and phytoplankton quantitative samples is the same as that of water quality sampling, see HJ 442.3; d) Qualitative phytoplankton samples and zooplankton samples are collected 2 m from the bottom and dragged vertically to the surface; e) The collection of macrobenthos shall be implemented in accordance with GB 17378.7. 5.2 Sample sampling a) Samples for each monitoring item are prepared and collected in accordance with GB 17378.7; b) Preparation before sampling. According to the survey items, locations and levels, equip with sufficient sample bottles, fixatives and other devices Use a suitable monitoring ship, the sampling operation must be carried out after the ship is stationary, according to the prevailing gas The sampling position can be adjusted appropriately for elephant and ocean current conditions to ensure the convenience and safety of sampling; c) Use sterile water collectors for microbial sampling, and pay attention to ensuring the aseptic operation of the entire process to avoid contamination; d) Plankton sampling uses shallow water type Ⅰ, Ⅱ, and Ⅲ plankton nets, and the netting does not exceed 1 m/s; the netting is about 0.5 m/s; e) Quantitative sampling of large benthic organisms uses a 0.1 m2 mud picker, 3 times at each point; in special circumstances, many For 2 times; if conditions do not permit, a 0.05 m2 mud picker can be used, but the number of sampling needs to be increased; qualitative sampling Use A's trawl, the trawling speed is controlled at about 2 knots, and the trawling time for each point is 10 min; f) Quantitative sampling in intertidal zone. sampling in high, middle and low tide areas, quantitative sampling device for tidal flat quantitative sampling, sample square The number is usually 8 per station (total 0.5 m2); if the beach sediment type is more uniform and the biological distribution is more uniform, 4 samples can be considered; quantitative sampling of rocky shore generally uses a 25 cm×25 cm quantitative frame, and 2 samples are taken from each station Sample. If the habitat density of organisms is very high and the distribution is relatively uniform, a quantitative frame of 10 cm×10 cm can be considered; g) Qualitative sampling in the intertidal zone. while sampling at each station, the species of animals and plants that appear near the station should be collected as much as possible. The collection is complete for reference during analysis, but the qualitative samples must be separated from the quantitative samples and should not be confused. 5.3 Sample storage and method Refer to GB 17378.7 for the fixation, storage and transportation of samples for monitoring items. The collection and storage of samples for monitoring items are often See Table 1 for usage method. a) Microbiological samples should be analyzed as soon as possible after collection, and the time should not exceed 2 hours, otherwise, the samples should be placed in an ice bottle or refrigerator In, the time shall not exceed 24 h; b) Chlorophyll a sample should be filtered immediately after collection, then wrap the filter membrane with aluminum foil, and dry it at -20℃ Dry and save for testing; c) The phytoplankton water sample is fixed by adding 6 ml to 8 ml Lugol's solution per liter of water sample (Lugol's solution is iodine tablets dissolved in Saturated solution prepared with 5% potassium iodide solution), shake well; add 5% formaldehyde solution to the net sample; Add formaldehyde solution to 5% of the sample volume of the net sample and shake well; d) After the zooplankton sample is collected, add a 5% formaldehyde solution with a volume fraction and shake well; e) After collecting the macrobenthic samples, they are cleaned with seawater on site, and the volume fraction for temporary storage is 5%～7% Neutral formaldehyde solution, 75% glycerol ethanol solution or 75% volume fraction for permanent storage Ethanol fixation; if the fixed sample has not been separated and identified for more than two months, the fixative should be replaced once.

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