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GB/T 44214-2024 English PDF

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GB/T 44214-2024: Test method for non-protein content in silk
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Basic data

Standard ID GB/T 44214-2024 (GB/T44214-2024)
Description (Translated English) Test method for non-protein content in silk
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard W40
Classification of International Standard 59.080.01
Word Count Estimation 14,123
Date of Issue 2024-07-24
Date of Implementation 2025-02-01
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 44214-2024: Test method for non-protein content in silk

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Test method for non-protein content in silk Test method for content of non-silk protein substances in silk ICS 59.080.01 CCS W 40 National Standard of the People's Republic of China Released on 2024-07-24 2025-02-01 Implementation State Administration for Market Regulation The National Standardization Administration issued

Table of Contents

Preface ... 1 Scope ... 2 Normative referenced documents ... 3 Terms and Definitions ... 4 Principle ... 5 Reagents and Materials ... 6 Instruments and Equipment ... 7 Test procedures ... 7.1 Preparation of standard solution ... Thai Silk Group Co., Ltd., Zhejiang Institute of Inspection and Quarantine, Hangzhou Quality and Technical Supervision Institute, Shanghai Quality Supervision Institute Inspection Technology Research Institute, Zhejiang Eco-textile Prohibited Dyes and Chemicals Testing Center Co., Ltd., Jiangsu Engineering Vocational and Technical College, SMIC Biotechnology (Hangzhou) Co., Ltd., Suzhou Intellectual Property Protection Center, Shaoxing Keqiao District China Textile City Inspection, Testing and Standardization Research Institute Research Institute. The main drafters of this document are. Wu Guojian, Wu Dongping, Chen Siyu, Sun Chenxiao, Qi Ning, Liu Yu, Jiang Zhaohui, Zhao Hailang, Chen Fengming, Liu Wei, Lei Bin, Ni Xuelin, Jin Feng, He Guojun, Wen Chaojun, Zhu Yangrui, Hang Zhiwei, Li Ting, Cao Shimiao, Chen Zhihua, Yin Guibo, Pan Lulu, Jiang Wei, Feng Guihua, Tong Jiajia. Test method for content of non-silk protein substances in silk

1 Scope

This document describes a test method for the determination of the content of non-silk protein substances in silk. This document is applicable to the calculation of silk by determining the content of four main amino acids (serine, glycine, alanine and tyrosine) in silk. The content of non-silk protein substances in it.

2 Normative references

The contents of the following documents constitute the essential clauses of this document through normative references in this document. For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies. in this document. GB/T 6682 Specifications and test methods for water used in analytical laboratories GB/T 8170 Rules for rounding off values and expression and determination of limit values GB/T 9995 Determination of moisture content and moisture regain of textile materials - Oven drying method

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Non-protein content The total amount of substances that have not been decomposed into amino acids after silk is hydrolyzed, including the amount of free NH4 that exceeds the normal range.

4 Principle

Silk is mainly composed of fibroin and sericin, which can be hydrolyzed into free amino acids in high-temperature acidic solutions. After reacting with phenyl isothiocyanate, The generated amino acid derivatives have strong absorption peaks at ultraviolet wavelengths. After separation by chromatographic column, high performance liquid chromatography was used to analyze the four The main amino acids (serine, glycine, alanine, tyrosine) are determined. Because the amino acid content in silk is relatively stable, The content of amino acids after hydrolysis of silk protein can be calculated to obtain the content of non-silk protein substances in silk. Note. Currently, the non-protein substances in silk mainly include grafted polymers with methacrylamide as the monomer.

5 Reagents and Materials

5.1 Concentrated hydrochloric acid. high purity. 5.2 Acetonitrile. chromatographic grade. 5.3 Phenyl isothiocyanate. purity ≥98%. 5.4 Triethylamine. analytical grade. 5.5 n-Hexane. chromatographic grade. 5.6 Acid hydrolysis solution. 6 mol/L hydrochloric acid (5.1) solution. 5.7 Phenyl isothiocyanate x acetonitrile solution (1 mol/L). Pipette 1.3 mL of phenyl isothiocyanate into a 10 mL volumetric flask and dilute to volume with acetonitrile.

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