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GB/T 44734-2024: Method for the determination of lectin in fungi - High performance liquid chromatography
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 44734-2024 | English | 169 |
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Method for the determination of lectin in fungi - High performance liquid chromatography
| Valid |
GB/T 44734-2024
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Standard similar to GB/T 44734-2024 GB/T 51033 GB/T 50082 Basic data | Standard ID | GB/T 44734-2024 (GB/T44734-2024) | | Description (Translated English) | Method for the determination of lectin in fungi - High performance liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B30 | | Classification of International Standard | 65.020.99 | | Word Count Estimation | 8,881 | | Date of Issue | 2024-10-26 | | Date of Implementation | 2024-10-26 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 44734-2024: Method for the determination of lectin in fungi - High performance liquid chromatography
---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT44734-2024
ICS 65.020.99
CCSB30
National Standard of the People's Republic of China
Determination of lectins in edible fungi by high performance liquid chromatography
Released on October 26, 2024
Implementation on October 26, 2024
State Administration for Market Regulation
The National Standardization Administration issued
Preface
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed and coordinated by the China National Institute of Standardization.
This document was drafted by. Institute of Process Engineering, Chinese Academy of Sciences, Lijiang Zhongyuan Green Food Co., Ltd., Shenzhen Advanced Quality Management Co., Ltd.
Technology Research Institute, Yunnan Yunke Specialty Plant Extraction Laboratory Co., Ltd., Rizhao Quality Inspection and Testing Institute, Hebei Zhongke Hanxi Biological
Technology Co., Ltd., Shaanxi Kangmeida Biotechnology Co., Ltd., China National Institute of Standardization, Tiansheng Pharmaceutical Group Chongqing Pharmaceutical Research Institute Co., Ltd.
Company, Beijing University of Chemical Technology, China Agricultural University, Zhongke Jiuban (Xi'an) Biotechnology Co., Ltd., Tianjin Zhiliao Technology Co., Ltd., Pony
Testing Group Co., Ltd., Laiyang Inspection and Testing Center, SGS-CSTC Standards Technical Services (Qingdao) Co., Ltd., Zhongshan Food Society,
Pharmaceutical and Environmental Testing Institute (Shandong) Group Co., Ltd., Liheweidao (Qingdao) Food Industry Co., Ltd.
The main drafters of this document are. Kong Yingjun, Zhang Guifeng, Zhan Songkun, Kang Jiyao, Wang Peng, Liu Minggai, Peng Zonglin, Ma Xiao, Li Xuguang, Ouyang Jing,
Liu Shuang, Wu Jiye, Yao Zhiyi, Liu Xinhao, Liu Lu, He Pei, Xi Xingjun, Lan Tao, Wei Yun, Liu Cong, Liu Yuanyuan, Qiu Jiquan, Shi Shen, He Yan,
Tan Penggang, Yao Yawei, Fu Yu, Zhang Yanjie, Pan Gang, Zhang Weixing, Liu Xiaomei, Sun Xuewen, Wang Jian, and Sun Shaoli.
Determination of lectins in edible fungi by high performance liquid chromatography
1 Scope
This method describes a high performance liquid chromatography (HPLC) method for the determination of lectin content in edible fungi.
This document is applicable to the determination of lectins in Enoki mushroom, Oyster mushroom, White jade mushroom, Lentinus edodes, and King oyster mushroom, and is for reference in other types of edible fungi.
The detection limit of lectin in this method is 6.2 mg/kg for fresh product and 9.2 mg/kg for dry product; the quantitative limit is 12.4 mg/kg for fresh product and 12.4 mg/kg for dry product.
The drug is 18.4 mg/kg.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
3 Terms and Definitions
There are no terms or definitions that require definition in this document.
4 Principles
The lectin in the sample was extracted with phosphate buffer, and the solubility and molecular weight differences were utilized to precipitate the lectin through ammonium sulfate and concentrate it by ultrafiltration.
The product was determined by high performance liquid chromatography, with retention time as the qualitative method and external standard method as the quantitative method.
5 Reagents and Materials
Unless otherwise specified, only analytical grade reagents were used.
5.1 Water, GB/T 6682, Grade 1.
5.2 Acetonitrile (C2H3N). chromatographically pure.
5.3 Trifluoroacetic acid (C2HO2F3).
5.4 Ammonium sulfate [(NH4)2SO4].
5.5 Sodium hydrogen phosphate solution (0.02 mol/L). Add 7.16 g of sodium hydrogen phosphate dodecahydrate (NaHPO4·
12H2O).
5.6 Sodium dihydrogen phosphate solution (0.02 mol/L). Add 3.12 g of sodium dihydrogen phosphate dihydrate (NaH2PO4·2H2O) to 1 L of first-grade water.
5.7 Phosphate extract (0.02 mol/L). Mix disodium hydrogen phosphate solution and sodium dihydrogen phosphate solution and adjust the pH to 7.2.
5.8 Ammonium sulfate solution (30% saturation). Weigh 176 g of ammonium sulfate [(NH4)2SO4] solid and dissolve it in grade 1 water to make up to 1 L.
5.9 Water filter membrane. pore size 0.45 μm, used for filtering mobile phase.
5.10 Lectins from edible fungi (electrophoretic purity ≥ 95%), or standards or samples certified by relevant authorities.
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