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GB/T 41906-2022: Assay method of superoxide dismutase activity
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| GB/T 41906-2022 | English | 119 |
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Assay method of superoxide dismutase activity
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GB/T 41906-2022
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PDF similar to GB/T 41906-2022
Standard similar to GB/T 41906-2022 GB/T 41907 GB/T 44828 Basic data | Standard ID | GB/T 41906-2022 (GB/T41906-2022) | | Description (Translated English) | Assay method of superoxide dismutase activity | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | C27 | | Classification of International Standard | 07.080 | | Word Count Estimation | 6,614 | | Date of Issue | 2022-10-14 | | Date of Implementation | 2022-10-12 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 41906-2022: Assay method of superoxide dismutase activity---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Assay method of superoxide dismutase activity
ICS 07.080
CCSC27
National Standards of People's Republic of China
Superoxide dismutase activity detection method
Published on 2022-10-12
2022-10-12 Implementation
State Administration for Market Regulation
Released by the National Standardization Administration
directory
Preface I
Introduction II
1 Scope 1
2 Normative references 1
3 Terms and Definitions 1
4 Principle 1
5 Reagents or materials 1
6 Instruments 2
7 Sample preparation 2
8 Test Step 2
9 Result calculation 3
10 Quality Control 3
foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
drafted.
Please note that some content of this document may be patented. The issuing agency of this document assumes no responsibility for identifying patents.
This document is proposed and managed by the National Tool Enzyme Standardization Working Group (SAC/SWG11).
This document is drafted by. Leigu (Xiamen) Biopharmaceutical Co., Ltd., Xiahe (Shenzhen) Biotechnology Co., Ltd., Guangdong Zhongding Testing
Technology Co., Ltd., Anhui Public Inspection Research Institute Co., Ltd., Bengbu Product Quality Supervision and Inspection Research Institute, Suzhou Kunqi Biotechnology Co., Ltd.
Co., Ltd., Shenzhen Hudaguao Biotechnology Co., Ltd., Fujian Nansheng Technology Co., Ltd., Shanghai Yingzi Life Technology Co., Ltd., Fujian
Huacan Pharmaceutical Co., Ltd., Shandong University, Fujian Agriculture and Forestry University, Fudan University, Suzhou Beaver Biomedical Engineering Co., Ltd., Xiamen University,
Shanghai Chuyu Biotechnology Co., Ltd., Wuhan University of Science and Technology, Institute of Metrology and Analysis of China Institute of Metrology.
The main drafters of this document. Huang Facan, Zheng Dengzhong, Wang Tongzhen, Tong Chengliang, Zhao Yi, Tang Qingwen, Feng Xiang, Liu Bin, Zhong Jiang, Zhu Li, Yao Juan,
Ren Hui, Zhang Yongyou, Xing Zhigang, Yang Zhonghua, Quan Can.
Introduction
Superoxide dismutase (EC1.15.1.1, referred to as SOD) is a metalloenzyme widely present in aerobic microorganisms, animals and plants, which can catalyze
The superoxide anion radical undergoes a disproportionation reaction. 2O2·- 2H → H2O2 O2.SOD maintains superoxide anion freedom in living organisms
It plays an important role in the dynamic balance of base generation and elimination. To formulate national standards for the detection method of superoxide dismutase activity to promote the
The industrialization of tool-like enzymes is of great significance for the production and use of superoxide dismutase.
Superoxide dismutase activity detection method
1 Scope
This document describes the principles, reagents or materials, equipment, sample preparation, test procedures,
Results calculation and quality control.
This document applies to the detection of superoxide dismutase activity.
2 Normative references
The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, dated citations
documents, only the version corresponding to that date applies to this document; for undated references, the latest edition (including all amendments) applies to
this document.
GB/T 6682 Analysis Laboratory Water Specifications and Experimental Methods
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
superoxide dismutase
Metalloenzymes are widely present in aerobic microorganisms, animals and plants, and can catalyze the disproportionation of superoxide anion free radicals.
Note. SOD plays an important role in maintaining the dynamic balance of the production and elimination of superoxide anion free radicals in organisms.
3.2
At 25°C, the amount of enzyme that inhibits the auto-oxidation rate of pyrogallol by 50% per minute in 1 mL of the reaction solution is one superoxide dismutase activity.
unit (U).
4 Principles
Pyrogallol is stable in an acidic environment, but will undergo auto-oxidation in a weakly alkaline environment, usually when a molecule of oxygen is completely reduced.
4 electrons generate 2O2- to further generate water. While pyrogallol only accepts one electron during auto-oxidation to generate superoxide anion free
base, and produce colored intermediates at a certain rate during its auto-oxidation process. SOD can disproportionate O2- into H2O2 and
O2, thereby inhibiting the auto-oxidation rate of pyrogallol, so the activity of the enzyme can be indirectly estimated based on the ability of SOD to decompose O2.
5 Reagents or materials
Unless otherwise specified, the reagents used are of analytical grade, and the water is secondary water specified in GB/T 6682.
5.1 A solution. 0.1moL/L hydrochloric acid solution
Measure 4.166mL of hydrochloric acid, add water to make up to 500mL.
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