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GB/T 41716-2022 English PDF

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GB/T 41716-2022: Determination of main active ingredient in lacquer trees - High performance liquid chromatography
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Basic data

Standard ID GB/T 41716-2022 (GB/T41716-2022)
Description (Translated English) Determination of main active ingredient in lacquer trees - High performance liquid chromatography
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B60
Classification of International Standard 65.020
Word Count Estimation 11,148
Date of Issue 2022-10-12
Date of Implementation 2023-11-01
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 41716-2022: Determination of main active ingredient in lacquer trees - High performance liquid chromatography


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Determination of main active ingredient in lacquer trees - High preformance liquid chromatography ICS 65.020 CCSB60 National Standards of People's Republic of China Determination of Main Active Components in Lacquer HPLC Released on 2022-10-12 2023-11-01 Implementation State Administration for Market Regulation Released by the National Standardization Management Committee

foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules for Standardization Documents" drafting. Please note that some contents of this document may refer to patents. The issuing agency of this document assumes no responsibility for identifying patents. This document is proposed by the State Forestry and Grassland Administration. This document is under the jurisdiction of the National Forest Chemical Products Standardization Technical Committee (SAC/TC558). This document is drafted by. Institute of Forestry Chemical Industry, Chinese Academy of Forestry, China National Institute of Standardization, Beijing Forestry University, South Beijing Forestry University, Shenzhen Institute of Standard Technology, Zhejiang University of Science and Technology, Xi'an Raw Lacquer Coating Research Institute of China National Supply and Marketing Cooperative Federation, Zhongshan Neide Agricultural Technology Co., Ltd. The main drafters of this document. Li Wenjun, Wang Chengzhang, Wang Yongmei, Liu Junli, Yang Zhongzhi, Zhou Hao, Chen Hongxia, Xi Xingjun, Lei Jiandu, Huang Chaobo, Yang Zhihua, Liu Tiebing, Lan Tao, Li Dongxu, Ren Hongyan, Li Yan, Shi Shanling. Determination of Main Active Components in Lacquer HPLC

1 Scope

This document describes a method for the determination of the main active ingredients in sumac by high performance liquid chromatography. This document is applicable to urushiol (saturated urushiol, monounsaturated urushiol, diunsaturated urushiol, triunsaturated and urushiol) and flavonoids (flavonoids, fisetin, thiamethrin and buterin) compounds for the determination of single or multiple components.

2 Normative references

The contents of the following documents constitute the essential provisions of this document through normative references in the text. Among them, dated references For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 36055 Determination of Moisture Content in Analytical Methods of Forestry Biomass Raw Materials

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 saturated urushiol saturatedurshiol The urushiol molecular compound that does not contain carbon-carbon double bonds in the side chain aliphatic alkanes of urushiol molecules. 3.2 The urushiol molecular compound with a carbon-carbon double bond in the side chain aliphatic alkanes of the urushiol molecule. 3.3 The urushiol molecular compound with two carbon-carbon double bonds in the side chain aliphatic alkanes of the urushiol molecule. 3.4 The urushiol molecular compound with three carbon-carbon double bonds in the side chain aliphatic alkanes of the urushiol molecule.

4 principles

In the same analysis cycle, adjust the mobile phase ratio according to a certain time program, so that the main active components in sumac can be adjusted according to their respective appropriate capacities. Factors achieve good separation purposes. Using saturated urushiol, monounsaturated urushiol, diunsaturated urushiol, triunsaturated urushiol, yellow pigment lignin, lacquer Flavin, thiochrysin and butacein have maximum ultraviolet absorption at specific wavelengths, and are detected under the conditions of corresponding wavelengths, and are tested with standard substances. Direct quantitative analysis by external standard method.

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