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GB/T 40833-2021: Determination of p-coumaric acid in sugarcane rinds - High performance liquid chromatography
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Determination of p-coumaric acid in sugarcane rinds - High performance liquid chromatography
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GB/T 40833-2021
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Basic data | Standard ID | GB/T 40833-2021 (GB/T40833-2021) | | Description (Translated English) | Determination of p-coumaric acid in sugarcane rinds - High performance liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B30 | | Word Count Estimation | 7,715 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 40833-2021: Determination of p-coumaric acid in sugarcane rinds - High performance liquid chromatography
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Determination of p-coumaric acid in sugarcane rinds - High performance liquid chromatography
ICS 67.080.01
CCSB30
National Standards of People's Republic of China
Detection method of coumaric acid in bagasse
High performance liquid chromatography
Released on 2021-10-11
2022-05-01 implementation
State Administration for Market Regulation
Issued by the National Standardization Management Committee
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents.
This document was proposed and managed by the China National Institute of Standardization.
Drafting organizations of this document. Beijing University of Chemical Technology, Shenzhen Institute of Standards and Technology, Beijing Electronics Technology Vocational College, China Standardization Research
Institute, Food and Drug Environmental Inspection Research Institute (Shandong) Group Co., Ltd., China Agricultural University, Dalian Yilite Analytical Instrument Co., Ltd., Tianjin Alta
Technology Co., Ltd., China Testing Testing and Certification Group Co., Ltd., Zhongshan Neide Agricultural Technology Co., Ltd., Jikesi Agricultural Technology (Xuzhou)
Limited company.
The main drafters of this document. Wei Yun, Xi Xingjun, Yang Zhihua, Lan Tao, Ni Hanwen, Sun Xuewen, Che Fenfang, Tang Tao, Xin Xiulan, Zhao Xinxin,
Zhang Teng, Xie Jun, Liu Tao, Zhang Lei, Liu Cong, Sun Yu, Liu Jinling, Xu Yin, Shi Shanling.
Detection method of coumaric acid in bagasse
High performance liquid chromatography
1 Scope
This document describes the high performance liquid chromatography method for the determination of coumaric acid in bagasse.
This document is applicable to the qualitative and quantitative determination of coumaric acid in bagasse. Refer to the content detection of products containing coumaric acid
use.
The detection limit of this document is 26.8mg/g, and the limit of quantification is 89.2mg/g.
2 Normative references
The content of the following documents constitutes an indispensable clause of this document through normative references in the text. Among them, dated quotations
Only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
This document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 6379.2 Accuracy of measurement methods and results (accuracy and precision) Part 2.Determine the repeatability of standard measurement methods
Basic methods of sex and reproducibility
3 Terms and definitions
There are no terms and definitions that need to be defined in this document.
4 Principle
The bagasse sample is extracted with 2mol/L NaOH solution, the pH is adjusted to about 2.0 with 6mol/L HCl solution, and ethyl acetate is extracted.
After being taken, it was separated by C18 chromatographic column, detected by ultraviolet-visible light detector, chromatographic peak retention time was qualitative, and external standard method was used for quantification.
5 Reagents and materials
5.1 Reagents
Unless otherwise stated, all reagents are chromatographically pure, and the water is the first grade water specified in GB/T 6682.
5.1.1 Methanol (chromatographically pure).
5.1.2 Acetic acid (chromatographically pure).
5.1.3 Ethyl acetate (chromatographically pure).
5.1.4 Hydrochloric acid (high-grade pure 36%~38%).
5.1.5 Sodium hydroxide (analytical grade).
5.2 Reagent preparation
Acetic acid aqueous solution (0.1%). Pipette 0.25mL acetic acid (5.1.2) into a 250mL volumetric flask, and dilute to the mark with water.
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