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GB/T 40170-2021: Principle of plasmid extraction and testing
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| GB/T 40170-2021 | English | 339 |
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Principle of plasmid extraction and testing
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GB/T 40170-2021
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Basic data | Standard ID | GB/T 40170-2021 (GB/T40170-2021) | | Description (Translated English) | Principle of plasmid extraction and testing | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A40 | | Word Count Estimation | 18,137 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 40170-2021: Principle of plasmid extraction and testing---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Principle of plasmid extraction and testing
ICS 07.080
CCSA40
National Standards of People's Republic of China
General Rules for Plasmid Extraction and Testing
Released on 2021-05-21
2021-12-01 implementation
State Administration of Market Supervision and Administration
Issued by the National Standardization Management Committee
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
This document was proposed and managed by the National Standardization Technical Committee for Biochemical Testing (SAC/TC387).
Drafting organizations of this document. Institute of Biology, China Academy of Testing Technology, General Biological System (Anhui) Co., Ltd., Shenzhen Analysis and Testing
Test Association, Gansu Zhongshang Food Quality Inspection and Testing Co., Ltd., Shenzhen Second People’s Hospital, Chengdu Institute of Food and Drug Inspection, Gansu
Provincial Commercial Science and Technology Research Institute Co., Ltd.
The main drafters of this document. Zhou Lihua, Yong Jingui, Wang Lina, Yu Mingjun, Cui Kangle, Ma Lixia, Luo Xiaowen, Jiang Zijing, Wang Weikun,
Gu Dayong, Ye Deping, Yang Jie, He Haining.
General Rules for Plasmid Extraction and Testing
1 Scope
This document specifies the terms and definitions, abbreviations, principles, extraction, testing, quality control, sample preservation, and reporting of plasmid extraction and testing
And waste disposal.
This document is suitable for the extraction and testing of plasmids.
2 Normative references
The contents of the following documents constitute indispensable clauses of the documents through normative references in the text. Among them, the dated reference documents, only
The version corresponding to this date is applicable to this document; for undated references, the latest version (including all amendments) is applicable to this document.
GB 4789.3 National Food Safety Standard Food Microbiological Inspection Coliform Count
GB/T 19495.1 General requirements and definitions for the testing of genetically modified products
GB/T 19495.2 Technical requirements for genetically modified products testing laboratories
GB/T 27403 Laboratory Quality Control Specification Food Molecular Biology Testing
GB/T 34265 Sanger method sequencing technology guide
GB/T 34796 Determination of the concentration and purity of nucleic acids in aqueous solutions by ultraviolet spectrophotometry
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Plasmid
One or more closed circular double-stranded DNA molecules other than chromosomes (or nucleoids) in organisms such as bacteria, yeasts, and actinomycetes.
Note. It exists in the cytoplasm and has the ability to replicate independently, enabling it to maintain a constant copy number in the progeny cells and express the genetic information it carries.
3.2
Ribonuclease A RNaseA
A kind of 3'and 5'phosphodiester bonds of the ribose moiety that catalyzes the hydrolysis of ribonucleic acid to form oligonucleosides with 2', 3'-cyclic monophosphate derivatives
Acid nuclease.
3.3
Bacterialendotoxin
A complex composed of hydrophilic heteropolysaccharides and a covalently bound lipid A.
Note. The complex is located on the cell wall of gram-negative bacteria.
Suction distress, intravascular coagulation and endotoxin shock syndrome.
4 Abbreviations
The following abbreviations apply to this document.
DNA. Deoxyribonucleic Acid (Deoxyribonucleic Acid)
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