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GB/T 40049-2021: PCR detection method of chicken salmonella enteritis
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PCR detection method of chicken salmonella enteritis
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GB/T 40049-2021
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Basic data | Standard ID | GB/T 40049-2021 (GB/T40049-2021) | | Description (Translated English) | PCR detection method of chicken salmonella enteritis | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Word Count Estimation | 16,164 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 40049-2021: PCR detection method of chicken salmonella enteritis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
PCR detection method of chicken salmonella enteritis
ICS 11.220
B41
National Standards of People's Republic of China
PCR Detection Method of Chicken Salmonella Enteritidis
Released on 2021-04-30
2021-11-01 implementation
State Administration of Market Supervision and Administration
Issued by the National Standardization Management Committee
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.
This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SC/TC181).
Drafting organizations of this standard. Shandong Agricultural University, Qingdao Agricultural University, China Center for Animal Health and Epidemiology, Shandong Academy of Agricultural Sciences
Poultry Research Institute, Qinhuangdao Customs, Shandong Parker Testing Technology Co., Ltd.
The main drafters of this standard. Chang Weishan, Sun Shuhong, Wang Shubai, Zhai Haihua, Cui Yanshun, Chai Tongjie, Ju Zijing, Zhao Xiaonan, Wang Tao, Huang Bing,
Song Minxun, Ma Xiuli, Gao Yuehua, Zheng Deyun, Guo Shuyuan, Ma Hongchao, Sun Jie, Zhang Fuyou.
PCR Detection Method of Chicken Salmonella Enteritidis
1 Scope
This standard specifies the PCR detection method for molecular typing of Salmonella enteritidis.
This standard is applicable to the PCR method detection of Salmonella enteritidis carried in chickens and their products of various ages.
2 Normative references
The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article
Pieces. For undated reference documents, the latest version (including all amendments) is applicable to this document.
GB 4789.4-2016 National Food Safety Standard Food Microbiological Inspection Salmonella Inspection
GB/T 6682-2008 Analytical laboratory water specifications and test methods
GB/T 28642-2012 Rapid detection method of Salmonella in feed polymerase chain reaction (PCR) method
NY/T 541-2016 Technical Specifications for Collection, Storage and Transportation of Veterinary Diagnostic Samples
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
House-keepinggene
A class of genes that are expressed in all types of cells in the physiology, pathology, and different developmental stages of an organism. Such genes are highly protected
Shou, gene products are essential for maintaining the basic structure and metabolic functions of cells.
4 Abbreviations
The following abbreviations apply to this document.
DNA. deoxyribonucleic acid (deoxyribonucleicacid)
PCR. polymerase chain reaction (polymerase chain reaction)
5 Reagents and materials
Unless otherwise specified, all reagents used are of analytical grade. The experimental water meets the GB/T 6682-2008 Grade II water regulations.
5.1 LB liquid medium (see A.1 in Appendix A for the preparation method).
5.2 Buffered peptone water (BPW) pre-enrichment solution (see A.2 for the preparation method).
5.3 Selenite cystine (SC) enrichment solution (see A.3 for the preparation method).
5.4 Tetrathiosulfonate brilliant green (TTB) bacteria enrichment solution (see A.4 for the preparation method).
5.5 Xylose Lysine Deoxycholate (XLD) Agar D.3 (see A.5 for the preparation method).
5.6 TAE running buffer (see A.6 for the preparation method).
5.7 Positive and negative control. The positive control is the standard strain of Salmonella enteritidis (CVCC3377) and the standard strain of Salmonella pullorum
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