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GB/T 38788-2020 English PDF

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GB/T 38788-2020: Technical specification for establishment of porcine pluripotent stem cells
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PDF similar to GB/T 38788-2020


Standard similar to GB/T 38788-2020

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Basic data

Standard ID GB/T 38788-2020 (GB/T38788-2020)
Description (Translated English) Technical specification for establishment of porcine pluripotent stem cells
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B43
Classification of International Standard 65.020.30
Word Count Estimation 10,125
Date of Issue 2020-04-28
Date of Implementation 2020-11-01
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 38788-2020: Technical specification for establishment of porcine pluripotent stem cells

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Technical specification for establishment of porcine pluripotent stem cells) ICS 65.020.30 B43 National Standards of People's Republic of China Technical specification for establishment of porcine pluripotent stem cells 2020-04-28 release 2020-11-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by China National Institute of Standardization. This standard was drafted by. China Agricultural University, Beijing Institute of Animal Science and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Northeast Agricultural University, Huazhong Agricultural University And China National Institute of Standardization. The main drafters of this standard. Han Jianyong, Zhang Jinying, Zhi Minglei, Liu Zhonghua, Miao Yiliang, Liu Zhiguo, Li Kui, Mou Yulian, Ma Aijin. Technical specification for establishment of porcine pluripotent stem cells

1 Scope

This standard specifies the general requirements, technical requirements, and verification methods for the establishment of pig pluripotent stem cell lines. This standard applies to the establishment of pig induced pluripotent stem cells and pig embryonic stem cells.

2 Normative references

The following documents are essential for the application of this document. For dated references, only the dated version applies to this article Pieces. For the cited documents without date, the latest version (including all amendments) applies to this document. GB/T 6682 Analysis laboratory water specifications and test methods GB 19489 General requirements for laboratory biosecurity

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Stem cell stemcel A type of cell that can renew itself, has multi-directional differentiation potential, and can differentiate into a variety of cell types. 3.2 Pluripotentstemcel Stem cells that can differentiate into many types of cells, including embryonic stem cells, nuclear transfer embryonic stem cells, induced pluripotent stem cells, etc. 3.3 Embryonic stem cells The initial undifferentiated cells derived from the inner cell mass in the early embryo can self-renew in vitro "without limitation", and have Stem cells with cell differentiation potential. 3.4 Induced pluripotentstemcel A type of stem cells that are artificially induced by cell reprogramming technology and have characteristics similar to embryonic stem cells. 3.5 Nuclear-transferred embryo-nuclear-transferredembryo The micronucleus is used to inject the nucleus into the enucleated oocytes, and the embryos are developed in vitro. 3.6 Parthenogenetic activated dembryo Mature oocytes in the middle of the second meiosis do not undergo fertilization by sperm, but cleavage occurs under the stimulation of physical and chemical factors The bred embryo. 3.7 In vitro fertilization embryo invitrofertilizedembryo Sperm and egg are fertilized and developed into embryos in an artificially controlled environment in vitro.

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