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GB/T 38578-2020: Rapid determination of the allergenic protein from aquatic animals origin - Capillary electrophoresis
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Rapid determination of the allergenic protein from aquatic animals origin - Capillary electrophoresis
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GB/T 38578-2020
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PDF similar to GB/T 38578-2020
Standard similar to GB/T 38578-2020 GB/T 38485 GB/T 38484 GB/T 38568 Basic data | Standard ID | GB/T 38578-2020 (GB/T38578-2020) | | Description (Translated English) | Rapid determination of the allergenic protein from aquatic animals origin - Capillary electrophoresis | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A21 | | Classification of International Standard | 07.080 | | Word Count Estimation | 8,829 | | Date of Issue | 2020-03-31 | | Date of Implementation | 2020-03-31 | | Quoted Standard | GB/T 6682; GB/T 30891 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | | Summary | This standard specifies the method for the determination of allergenic proteins from aquatic products by capillary electrophoresis. This standard applies to the rapid detection of crustacean tropomyosin, crustacean arginine kinase and fish parvalbumin content. | GB/T 38578-2020: Rapid determination of the allergenic protein from aquatic animals origin - Capillary electrophoresis
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Rapid determination of the allergenic protein from aquatic animals origin--Capillary electrophoresis
ICS 07.080
A21
National Standards of People's Republic of China
Rapid detection of allergenic proteins of aquatic origin
Capillary electrophoresis
2020-03-31 release
2020-03-31 Implementation
State Administration of Market Supervision and Administration
Issued by the National Standardization Management Committee
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by China National Institute of Standardization.
This standard was drafted by. China National Institute of Standardization, Greentown Agricultural Testing Technology Co., Ltd., Zhejiang Technology and Business University, Beijing Technology and Business University,
Beijing Sambe Technology Co., Ltd., Jimei University, Hunan Agricultural University, Wenzhou University, Rongcheng Huatong Marine Biotechnology Co., Ltd.
The main drafters of this standard. Ma Aijin, Wang Chuanpi, He Xiaoming, Zhou Jinru, Fu Linglin, Jia Yingmin, Wu Qi, Hao Shuai, Liu Guangming, Zhou Hui,
Chen Xiaojing, Wang Chong, Liu Yixiang, Liu Zhongfu, Wang Yanbo.
Rapid detection of allergenic proteins of aquatic origin
Capillary electrophoresis
1 Scope
This standard specifies the method for determination of aquatic allergenic proteins by capillary electrophoresis.
This standard applies to the rapid detection of the content of crustaceous myosin, crustaceous arginine kinase and fish albumin.
2 Normative references
The following documents are essential for the application of this document. For dated references, only the dated version applies to this article
Pieces. For the cited documents without date, the latest version (including all amendments) applies to this document.
GB/T 6682 Analysis laboratory specifications and test methods
GB/T 30891 Standard for Sampling of Aquatic Products
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Allergenic protein
A protein that can cause an allergic reaction in the body.
4 Principle
The allergenic protein in the sample is extracted by protein extract and purified by anion exchange chromatography, measured by capillary electrophoresis, migration time
Qualitative, quantitative by external standard method.
5 Reagents or materials
Unless otherwise specified, only analytical reagents are used.
5.1 Water
Grade 1 water specified in GB/T 6682.
5.2 Methanol
Chromatographically pure.
5.3 Tris-hydroxymethylaminomethane-hydrochloric acid buffer
Trimethylolaminomethane 1.21g, add 900mL of double-distilled water to dissolve, adjust the pH to 7.5 with 6mol/L hydrochloric acid solution, set with distilled water
Make up to 1000mL, filter with 0.45μm filter membrane, ultrasonically shake (power.200W) for 30min, and store at room temperature.
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