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GB/T 38577-2020 English PDF

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GB/T 38577-2020: Determination of the biological activity for plant hormone-related secondary metabolites - Indicator plant method
Status: Valid

Standard ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status	PDF
GB/T 38577-2020	English	209	Add to Cart	3 days [Need to translate]	Determination of the biological activity for plant hormone-related secondary metabolites - Indicator plant method	Valid	GB/T 38577-2020

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Basic data

Standard ID	GB/T 38577-2020 (GB/T38577-2020)
Description (Translated English)	Determination of the biological activity for plant hormone-related secondary metabolites - Indicator plant method
Sector / Industry	National Standard (Recommended)
Classification of Chinese Standard	A21
Classification of International Standard	07.080
Word Count Estimation	11,120
Date of Issue	2020-03-31
Date of Implementation	2020-03-31
Quoted Standard	GB/T 6682
Issuing agency(ies)	State Administration for Market Regulation, China National Standardization Administration
Summary	This standard specifies the method for determining the biological activity of phytohormones secondary metabolites by the indicator plant method. This standard applies to the determination of the activity of auxin, cytokinin and gibberellin of the secondary metabolites of plant hormones.

GB/T 38577-2020: Determination of the biological activity for plant hormone-related secondary metabolites - Indicator plant method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of the biological activity for plant hormone-related secondary metabolites--Indicator plant method ICS 07.080 A21 National Standards of People's Republic of China Determination of biological activity of secondary metabolites of plant hormones Indicator plant law 2020-03-31 release 2020-03-31 Implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by China National Institute of Standardization. This standard was drafted by. China Jiliang University, China Institute of Standardization, Zhejiang Academy of Inspection and Quarantine Science and Technology. The main drafters of this standard. Zhang Yafen, Ye Zihong, Huang Chaoqun, Ma Aijin, Yu Xiaoping, Shen Tu Xuping, Xu Yipeng, Cui Haifeng, Li Yi, Chen Li and Wu Juan. Determination of biological activity of secondary metabolites of plant hormones Indicator plant law

1 Scope

This standard specifies the method for determining the biological activity of phytohormone secondary metabolites by the indicator plant method. This standard applies to the determination of the activity of plant hormones secondary metabolites auxin, cytokinin and gibberellin.

2 Normative references

The following documents are essential for the application of this document. For dated references, only the dated version applies to this article Pieces. For the cited documents without date, the latest version (including all amendments) applies to this document. GB/T 6682 Analysis laboratory specifications and test methods 3 Terms, definitions and abbreviations 3.1 Terms and definitions The following terms and definitions apply to this document. 3.1.1 Planthormone-related secondary metabolites Activities from plants themselves synthesized and obtained by microbial fermentation or chemical synthesis to regulate plant growth, development and dormancy substance. 3.1.2 Biological activity The equal concentration of phytohormone secondary metabolites and their corresponding standards promote or inhibit plant growth, development and dormancy relative value. 3.2 Acronyms The following abbreviations apply to this document. GA3. Gibberellic acid (gibberelicacid) NAA. 1-naphthylacetic acid ZT. Zeatin

4 Principle

Within a certain concentration range, the concentration of phytohormone active substances and the specific tissue growth or specific substances that promote the corresponding indicator plants The ability to synthesize is proportional. Using the standard NAA as a reference, according to the linear regression of the relative elongation of wheat coleoptile and the logarithm of NAA concentration The standard curve is obtained from the relationship, and the ratio of the NAA concentration to the sample concentration that makes the relative elongation of the coleoptile in the linear range is calculated, Represents the auxin activity of the 1mg/mL sample; taking the standard ZT as a reference, according to the synthesis amount of amaranthin and ZT in the cotyledons of Amaranthus tricolor

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