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GB/T 38571-2020: Determination of alkaloids as plant secondary metabolites - Liquid chromatography-mass spectrometry
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 38571-2020 | English | 209 |
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Determination of alkaloids as plant secondary metabolites - Liquid chromatography-mass spectrometry
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GB/T 38571-2020
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PDF similar to GB/T 38571-2020
Standard similar to GB/T 38571-2020 GB/T 38485 GB/T 38484 GB/T 38568 Basic data | Standard ID | GB/T 38571-2020 (GB/T38571-2020) | | Description (Translated English) | Determination of alkaloids as plant secondary metabolites - Liquid chromatography-mass spectrometry | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A21 | | Classification of International Standard | 07.080 | | Word Count Estimation | 11,118 | | Date of Issue | 2020-03-31 | | Date of Implementation | 2020-03-31 | | Quoted Standard | GB/T 6682 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | | Summary | This standard specifies the method for the detection of plant secondary metabolite alkaloids by liquid chromatography-tandem mass spectrometer. This standard applies to (-)-scopolamine, scopolamine, aconitine, hypoconitine, neoaconitine, colchicine, atropine, strychnine, camptothecin, pilocarpine, podophyllin Determination of toxins. | GB/T 38571-2020: Determination of alkaloids as plant secondary metabolites - Liquid chromatography-mass spectrometry
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of alkaloids as plant secondary metabolites--Liquid chromatography-mass spectrometry
ICS 07.080
A21
National Standards of People's Republic of China
Detection of plant secondary metabolite alkaloid
Liquid chromatography-mass spectrometry
2020-03-31 release
2020-03-31 Implementation
State Administration of Market Supervision and Administration
Issued by the National Standardization Management Committee
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by China National Institute of Standardization.
This standard was drafted by. Institute of Biology, China Institute of Testing Technology, Hebei Food Inspection Institute, China Institute of Standardization,
Hebei Medical University, China Jiliang University.
The main drafters of this standard. Zhang Yan, Fan Sufang, Ma Aijin, Feng Dejian, Ma Chunling, Wen Di, Cong Bin, Wang Juan, Lu Pin, Ye Zihong.
Detection of plant secondary metabolite alkaloid
Liquid chromatography-mass spectrometry
1 Scope
This standard specifies the method for the detection of plant secondary metabolite alkaloids by liquid chromatography-tandem mass spectrometry.
This standard applies to (-)-scopolamine, papaverine, aconitine, hypoaconitine, neoaconitine, colchicine, atropine, strychnine, hi
Determination of dendropine, pilocarpine, podophyllotoxin.
2 Normative references
The following documents are essential for the application of this document. For dated references, only the dated version applies to this article
Pieces. For the cited documents without date, the latest version (including all amendments) applies to this document.
GB/T 6682 Analysis laboratory specifications and test methods
3 Principle
After ultrasonic extraction and centrifugation, the samples were measured by liquid chromatography-tandem mass spectrometry and quantified by external standard method.
4 Reagents or materials
Unless otherwise specified, the reagents used are of analytical grade. The water is the first grade water specified in GB/T 6682.
4.1 10% (volume fraction) acetonitrile solution
10mL acetonitrile 90mL deionized water, mix well.
4.2 Formic acid
Chromatographically pure.
4.3 0.1% (volume fraction) formic acid aqueous solution
Accurately transfer 1.0mL of formic acid into a 1L volumetric flask, make up to volume with water, and mix well.
4.4 1% (volume fraction) formic acid-acetonitrile solution
1mL formic acid 99mL acetonitrile, mix well.
4.5 100mmol/L ammonium acetate solution
Weigh 3.854g of ammonium acetate, dissolve in deionized water, and bring the volume to 500mL.
4.6 Ammonium acetate-ammonia buffer, pH9
Take 100mL100mmol/L ammonium acetate solution and adjust the pH to 9.0 ± 0.5 with ammonia water.
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