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GB/T 38570-2020 English PDF

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GB/T 38570-2020: Determination for ingredients of genetically modified plants - Target sequencing methods
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PDF similar to GB/T 38570-2020


Standard similar to GB/T 38570-2020

GB/T 38485   GB/T 38484   GB/T 38568   

Basic data

Standard ID GB/T 38570-2020 (GB/T38570-2020)
Description (Translated English) Determination for ingredients of genetically modified plants - Target sequencing methods
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard A21
Classification of International Standard 07.080
Word Count Estimation 39,381
Date of Issue 2020-03-31
Date of Implementation 2020-03-31
Quoted Standard GB/T 6682; GB/T 19495.2; GB/T 19495.3; GB/T 19495.5-2018; GB/T 19495.7; GB/T 27403
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration
Summary This standard specifies the method for the determination of plant transgenic components by target sequence sequencing. This standard applies to the qualitative detection of exogenous genes and transgenic lines in plants and their products. The qualitative lower limit of detection (LOD) of the foreign gene in this method is 0.1% (the copy number ratio between the foreign gene and the internal standard gene).

GB/T 38570-2020: Determination for ingredients of genetically modified plants - Target sequencing methods


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Determination for ingredients of genetically modified plants--Target sequencing methods ICS 07.080 A21 National Standards of People's Republic of China Determination of plant genetically modified components Target sequence sequencing 2020-03-31 release 2020-03-31 Implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Contents

Foreword Ⅲ 1 Scope 1 2 Normative references 1 3 Terms and definitions 1 4 Principle 2 5 Reagents or materials 2 6 Equipment 2 7 Measurement step 2 7.1 Sampling and sample preparation 2 7.2 DNA extraction and purification 2 7.3 Library Construction 2 7.4 High-throughput sequencing 3 8 Quality Control 3 9 Result analysis and presentation 3 9.1 Results analysis 3 9.2 Expression 4 10 Pollution prevention measures 4 Appendix A (Normative Appendix) Probe Sequence 5 Appendix B (Normative Appendix) Artificial DNA 36

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some content of this document may involve patents. The issuer of this document does not assume responsibility for identifying these patents. This standard is proposed and managed by China National Institute of Standardization. This standard was drafted by. Jianghan University, Wuhan Mingle Biotechnology Co., Ltd., China National Institute of Standardization, Beijing Technology and Business University, China Institute of Genetics and Developmental Biology, Academy of Sciences, Hunan Hybrid Rice Research Center, Shenzhen Huada Zhizao Technology Co., Ltd. The main drafters of this standard. Peng Hai, Chen Lihong, Fang Zhiwei, Zhang Jianan, Li Tiantian, Li Lun, Cui Yehan, Ma Aijin, Jia Yingmin, Chen Hong, Zhou Junfei, Zhai Wenxue, Xu Na, Liang Yong, Gao Lifen, Song Shufeng, Hu Meixia, Fu Xiqin, Zhang Jing, Yu Jinwen. Determination of plant genetically modified components Target sequence sequencing

1 Scope

This standard specifies the method for determining plant transgenic components by target sequence sequencing. This standard applies to the qualitative detection of foreign genes and transgenic lines in plants and their products. Qualitative detection of foreign genes in this method The lower limit (LOD) is 0.1% (copy number ratio between foreign genes and internal standard genes).

2 Normative references

The following documents are essential for the application of this document. For dated references, only the dated version applies to this article Pieces. For the cited documents without date, the latest version (including all amendments) applies to this document. GB/T 6682 Analysis laboratory specifications and test methods GB/T 19495.2 GM product testing laboratory technical requirements GB/T 19495.3 Method for extraction and purification of nucleic acid for detection of genetically modified products GB/T 19495.5-2018 Detection of genetically modified products Real-time fluorescence quantitative polymerase chain reaction (PCR) detection method GB/T 19495.7 Testing and sampling methods of genetically modified products GB/T 27403 Laboratory Quality Control Specification Food Molecular Biological Testing

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Endogenous referencegene Genes that are ubiquitous in different plant species and have constant copy numbers. 3.2 Exogenous gene Other biological genes transferred by bioengineering technology to make the biological variety show new biological traits. 3.3 Quality control sample The sample is prepared in parallel with the sample to be tested, which is used to show whether the sample to be tested is contaminated during the sample preparation process. 3.4 Templatefragment A template deoxyribonucleic acid (DNA) inferred from a set of sequencing fragments with the same random barcode in the high-throughput sequencing fragments sequence. 3.5 Characteristic fragment of exogenous gene Template fragments that contain foreign genes but do not exist in nature. Note. Including foreign gene sequences containing artificially modified sequences, and the sequence of the foreign gene and the exogenous gene other than the boundary sequence of the exogenous gene in the species of origin Connected sequence.

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