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GB/T 38506-2020: Determination of biochemical parameters in animal cell culture process
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 38506-2020 | English | 259 |
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Determination of biochemical parameters in animal cell culture process
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GB/T 38506-2020
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Basic data | Standard ID | GB/T 38506-2020 (GB/T38506-2020) | | Description (Translated English) | Determination of biochemical parameters in animal cell culture process | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A40 | | Classification of International Standard | 07.080 | | Word Count Estimation | 14,176 | | Date of Issue | 2020-03-06 | | Date of Implementation | 2020-03-06 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 38506-2020: Determination of biochemical parameters in animal cell culture process---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of biochemical parameters in animal cell culture process
ICS 07.080
A40
National Standards of People's Republic of China
Method for measuring biochemical parameters in animal cell culture
2020-03-06 release
2020-03-06 implementation
State Administration of Market Supervision
Published by the National Standardization Administration
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Technical Committee for Biochemical Testing Standardization (SAC/TC387).
This standard was drafted. China Institute of Testing Technology, Institute of Biology, Cinda Biopharmaceutical (Suzhou) Co., Ltd., Huapai Bioengineering
Group Co., Ltd., Shanghai Institute of Metrology and Testing Technology, Shanghai Zhangjiang Biotechnology Co., Ltd., Sichuan University, Chengdu Biotec Biotech
Technology Co., Ltd., Shanghai Maitai Junao Biotechnology Co., Ltd., Taizhou Maibo Taike Pharmaceutical Co., Ltd., Shanghai Baimaibo Pharmaceutical Co., Ltd.
Limited company.
The main drafters of this standard. Zhou Lihua, Zhou Kaisong, Song Hang, Qiu Wenying, Li Jianfeng, Liu Gang, Huang Lin, Ma Lixia, Li Zhenhua, Yang Kun, Li Yan,
Jiang Zijing, Fang Pengfei, Cui Likai, Hao Weiwei, Ye Deping, Liang Zhijie, Zhang Dapeng, Zhang Liyan, Guo Huaizu, Xu Jin, Guo Qingcheng, Dai Jianxin.
Method for measuring biochemical parameters in animal cell culture
1 Scope
This standard specifies cell density, cell viability, pH, dissolved oxygen, osmotic pressure, glucose content,
Method for determination of bacterial endotoxin and lactic acid.
This standard applies to Chinese hamster ovary cells (CHO), mouse myeloma cells (NSO, SP2/0), and African green monkey kidney cells.
(Vero), breast hamster kidney cells (BHK-21), canine kidney epidermal cells (MDCK), pig testicular cells (ST) during biochemical parameters
The detection of other biochemical parameters during the scale culture of eukaryotic cells can also be used for reference.
2 Normative references
The following documents are essential for the application of this document. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
WS/T 350 Reference Method for Determination of Serum Glucose
Pharmacopoeia of the People's Republic of China (2015 Edition) Part Two (National Pharmacopoeia Committee)
Pharmacopoeia of the People's Republic of China (2015 edition) three (National Pharmacopoeia Committee)
3 Terms and definitions, acronyms
3.1 Terms and definitions
The following terms and definitions apply to this document.
3.1.1
Cell density
After the cells pass the culture, the total number of cells in a unit volume sample.
3.1.2
Cell viability
In the cell culture process, the number of live cells as a percentage of the total number of cells.
3.2 Acronyms
The following abbreviations apply to this document.
ATP. Adenosinetriphosphate
NADH. reduced coenzyme I (nicotinamide adenine dinucleotide, reduced state) (Nicotinamide adenine dinucleotide)
NAD (P). Nicotinamide adenine dinucleotide phosphate
NAD (P) H. reduced coenzyme Ⅱ (nicotinamide adenine dinucleotide phosphate, reduced form) (Nicotinamideadenine
dinucleotidephosphate)
OPC. Open Platform Communications (OpenPlatformCommunications)
PBS. PhosphateBuffered Saline
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