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GB/T 38488-2021: Rapid determination of microorganisms
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Rapid determination of microorganisms
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GB/T 38488-2021
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Basic data | Standard ID | GB/T 38488-2021 (GB/T38488-2021) | | Description (Translated English) | Rapid determination of microorganisms | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A21 | | Word Count Estimation | 110,172 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 38488-2021: Rapid determination of microorganisms---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Rapid determination of microorganisms
ICS 07:080
CCSA21
National Standards of People's Republic of China
Microbial Rapid Determination Method
Published on 2021-12-31
2022-07-01 Implementation
State Administration for Market Regulation
Released by the National Standardization Administration
foreword
This document is in accordance with the provisions of GB/T 1:1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules of Standardization Documents"
drafted:
Please note that some content of this document may be patented: The issuing agency of this document assumes no responsibility for identifying patents:
This document is proposed and managed by China National Institute of Standardization:
This document is drafted by: Beijing Entry-Exit Inspection and Quarantine Bureau Inspection and Quarantine Technology Center, China National Institute of Standardization, Tianjin Customs Industrial Products
Product Safety Technology Center, Jianghan University, Beijing Technology and Business University, Wuhan Xudong Food Co:, Ltd:, Beijing Sambo Technology Co:, Ltd:, China Testing
Testing and Certification Group Beijing Co:, Ltd:, China Agricultural University:
The main drafters of this document: Zhang Jie, Ma Aijin, Liu Ming, Zhao Zhuo, Peng Hai, Chang Xiaohui, Zhang Yuan, Jia Yingmin, Gao Xin, Yang Xiangying, Zhang Huiyuan,
He Xudong, Hao Shuai, Li Xiaolin, Dong Liya, Zhang Hao, Wang Hua, Chen Guangquan, Peng Yankun:
Microbial Rapid Determination Method
1 Scope
This document specifies methods for the rapid determination of microorganisms using molecular motor methods, near-infrared immunochromatography, and target region sequencing:
The first method in this document, the molecular motor method, is applicable to Listeria monocytogenes (L:mono-
cytogenes), Vibrioparahemolyticus (Vibrioparahemolyticus), Vibriocholerae (Vibriocholerae), Salmonella (Salmonel-
la), Cronobacter sakazakii (Cronobactersakazakii), Shigella, Hepatitis A virus
Rapid determination of (HepatitisAvirus, HAV); the second method --- near-infrared immunochromatography is suitable for single in food and drinking water
Listeria nucleocytogenes, Vibrio parahaemolyticus, Vibrio cholerae, Salmonella, Rotavirus, Norovirus
virus), rapid determination of hepatitis A virus; the third method---target region sequencing method is suitable for Salmonella, Salmonella in food and drinking water
Shigella, Staphylococcusaureus, Vibrio parahaemolyticus, Yersinia enterocolitica
enterocolitica), Campylobacter jejuni, Listeria monocytogenes, Enterohemorrhagic Escherichia coli
O157:H7 (EnterohemorrhagicE:coliO157), Vibrio cholerae, Cronobacter sakazakii (Enterobacter sakazakii), Vibrio vulnificus
Rapid assay of Vibriovulnificus and Vibrioalginolyticus:
2 Normative references
The contents of the following documents constitute essential provisions of this document through normative references in the text: Among them, dated citations
documents, only the version corresponding to that date applies to this document; for undated references, the latest edition (including all amendments) applies to
this document:
GB/T 6682 Analysis Laboratory Water Specifications and Test Methods
3 Terms and Definitions
There are no terms and definitions that need to be defined in this document:
4 Molecular motor method
4:1 Principle
ATP synthase is a rotary molecular motor driven by a transmembrane proton (H ) gradient, the epsilon subunit on which is a rotor, and the rotor is negative:
The higher the load, the lower the rotational speed of the molecular motor and the lower the number of protons (H) transported across the membrane: Attaches the epsilon subunit to the epsilon subunit of ATP synthase
body-streptavidin-biotin-nucleic acid probe (see Appendix A, Table A:2), when the target pathogenic microorganism-specific gene sequence is detected and associated with it
When bound, the rotational speed of the molecular motor can be indirectly detected by pH-sensitive fluorescent probes labeled outside the chromatophore membrane:
1,2-bishexadecyl-3-glycero-phosphoethanolamine (DHPE) was used to detect the target pathogenic microorganisms:
4:2 Reagents and materials
Unless otherwise specified, only analytical grade reagents were used:
4:2:1 Water: Grade 1 water in GB/T 6682:
4:2:2 DNA extraction kit:
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