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GB/T 38137-2019: Guidelines for in vitro screening of nucleic acid aptamers
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Guidelines for in vitro screening of nucleic acid aptamers
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GB/T 38137-2019
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Basic data | Standard ID | GB/T 38137-2019 (GB/T38137-2019) | | Description (Translated English) | Guidelines for in vitro screening of nucleic acid aptamers | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A20 | | Classification of International Standard | 07.080 | | Word Count Estimation | 10,125 | | Date of Issue | 2019-10-18 | | Date of Implementation | 2019-10-18 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 38137-2019: Guidelines for in vitro screening of nucleic acid aptamers---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Guidelines for in vitro screening of nucleic acid aptamers
ICS 07.080
A20
National Standards of People's Republic of China
Guidelines for in vitro screening of nucleic acid aptamers
Published on.2019-10-18
2019-10-18 implementation
State market supervision and administration
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed and managed by the China National Institute of Standardization.
This standard was drafted. Hebei Food Inspection Institute, China National Institute of Standardization, University of Science and Technology of China, Hefei University of Technology, Hebei
Agricultural University, Southwest University, Jiangnan University, Henan University, Zhejiang Gongshang University, Beijing Food Science Research Institute, Hebei Medical University.
The main drafters of this standard. Luo Zhaofeng, Zhou Wei, Ma Aijin, Zhang Yan, Yan, Zheng Lei, Wang Xianghong, Chen Jia, Wang Zhouping, Chen Wei, Ma Liang,
Tian Yiling, Zhang Cuixia, Li Yongbo, Li Yongyan, Kang Wenyi, Wang Yanbo, Fu Linglin, Sun Yong, Lu Pin.
Guidelines for in vitro screening of nucleic acid aptamers
1 Scope
This standard specifies the screening process for screening of nucleic acid aptamers in vitro, screening process monitoring, sequencing and sequence analysis, and identification of candidate aptamers.
Set and optimize, anti-pollution measures.
This standard is applicable to different levels of target objects such as metal ions, small molecules, macromolecules, cells and particles, tissues and mixed targets.
Screening of nucleic acid aptamers.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 27403 Laboratory Quality Control Specification for Food Molecular Biology Testing
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Nucleic acid aptamer nucleicacidaptamer
An oligonucleotide fragment capable of high affinity and specific binding to a specific target.
Note. Targets can be metal ions, small molecules, macromolecules, cells, tissues, and mixed targets. The nucleic acid aptamer is folded into a specific three-dimensional structure,
The spatial conformation complements the high specific binding of the target with high affinity, and is mainly composed of a short single-stranded nucleotide sequence (single-stranded DNA or RNA).
3.2
In vitro screening technology invitroscreeningtechnique
The formed target-oligonucleotide complex and free oligonucleotide are formed by interaction of the target with a large-capacity random oligonucleotide library
Isolation, and then using the PCR in vitro amplification technique to amplify the oligonucleotide forming the complex, and repeating the above screening steps multiple times to finally obtain
A technique for nucleic acid aptamers that specifically bind to a target.
4 Abbreviations
The following abbreviations apply to this document.
PAGE. polyacrylamide gel electrophoresis;
PCR. polymerase chain reaction (polymerase chain reaction);
polyA. polyadenylation;
SELEX. Exponentially enriched ligand system evolution (exponentialyenrichedligandsystemevolution).
5 screening process
5.1 Screening basic ways
The basic approach of SELEX technology is to store large-capacity random oligonucleotide sequence libraries (1013~1015 random oligonucleotide sequences) with targets.
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