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GB/T 38133-2019 English PDF

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GB/T 38133-2019: Genetically modified alfalfa detection method by real-time PCR
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Basic data

Standard ID GB/T 38133-2019 (GB/T38133-2019)
Description (Translated English) Genetically modified alfalfa detection method by real-time PCR
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard A40
Classification of International Standard 07.080
Word Count Estimation 10,158
Date of Issue 2019-10-18
Date of Implementation 2019-10-18
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 38133-2019: Genetically modified alfalfa detection method by real-time PCR

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Genetically modified alfalfa detection method by real-time PCR ICS 07.080 A40 National Standards of People's Republic of China Transgenic 苜蓿 real-time fluorescent PCR detection method Published on.2019-10-18 2019-10-18 implementation State market supervision and administration China National Standardization Administration issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Biochemical Testing Standardization Technical Committee (SAC/TC387). This standard was drafted. China Inspection and Quarantine Science Research Institute, Guangzhou Customs Technology Center, Guangxi Entry-Exit Inspection and Quarantine Bureau inspection and quarantine Technical Center, Inspection and Quarantine Technology Center of Liaoning Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Fu Wei, Zhu Pengyu, Liu Jin, Wei Shuang, Du Zhixin, Wang Chenguang, Zhu Shuifang, Huang Wensheng, Zheng Qiuyue, Cao Jijuan. Transgenic 苜蓿 real-time fluorescent PCR detection method

1 Scope

This standard specifies a real-time fluorescent polymerase chain reaction (PCR) qualitative detection method for transgenic components. This standard is applicable to real-time fluorescent PCR detection of transgenic 苜蓿J101, J163 and KK179 strains in seeds and feeds.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB/T 19495.1 General requirements and definitions for the detection of genetically modified products GB/T 19495.2 Technical requirements for testing laboratory of genetically modified products GB/T 19495.3 Transgenic product detection nucleic acid extraction and purification method GB/T 19495.7 GM product testing sampling and sample preparation method

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 ACC gene acetylCoAcarboxylase A gene encoding an acetyl-CoA carboxylase. Note. As an internal standard gene of sputum in this standard. 3.2 18S ribosomal RNA 18SrRNA A gene encoding eukaryotic 18S ribosomal RNA. Note 1. In this standard, it is the internal standard gene of sputum. Note 2. In the actual test, 3.1 and 3.2 can be used as one. 3.3 FMV35S Promoter 35SpromoterfromamodifiedFigwortmosaicvirus The gene encoding the 35S promoter of Scrophulari Mosaic Virus. Note. As a promoter of the transgenic 苜蓿J101 and J163 in this standard. 3.4 E9 terminator 3'terminationregionfrompearibulose1,5-bisphosphatecarboxylase The 3' untranslated region derived from the pea ribulose-1,5-bisphosphate carboxylase small subunit (RbcS) E9 gene, present in the transgenic 苜 蓿 J101 and J163 strains. 3.5 CP4-EPSPS gene 5-enolpyruvylshikimate-3-phosphatesynthasegene From Agrobacterium CP4 strain, a gene encoding a key enzyme in the shikimate pathway, present in the transgenic 苜蓿J101 In the J163 strain.

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