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GB/T 38086-2019: Determination of metallothionein (MT) in biological samples
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| GB/T 38086-2019 | English | 279 |
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Determination of metallothionein (MT) in biological samples
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GB/T 38086-2019
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Basic data | Standard ID | GB/T 38086-2019 (GB/T38086-2019) | | Description (Translated English) | Determination of metallothionein (MT) in biological samples | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A40 | | Classification of International Standard | 07.080 | | Word Count Estimation | 14,142 | | Date of Issue | 2019-10-18 | | Date of Implementation | 2019-10-18 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 38086-2019: Determination of metallothionein (MT) in biological samples---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of metallothionein(MT) in biological samples
ICS 07.080
A40
National Standards of People's Republic of China
Determination of metallothionein content in biological samples
Published on.2019-10-18
2019-10-18 implementation
State market supervision and administration
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Biochemical Testing Standardization Technical Committee (SAC/TC387).
This standard was drafted. National Agricultural Standardization Monitoring and Research Center (Heilongjiang), Shenzhen Institute of Metrology and Quality Testing, Shanghai
Institute of Metrology and Testing Technology, Sichuan Institute of Antibiotics, Chengdu University, Technical Center of Jilin Provincial Inspection and Quarantine Bureau, Harbin University of Commerce
Food Institute, Harbin Chunyuan Biotechnology Development Co., Ltd.
The main drafters of this standard. Wang Zhiqiang, Wang Weida, Dong Shan, Zhou Wei, Du Yegang, Li Lanying, Yang Chen, Rong Hui, Zhang Gensheng, Zhang Fuyuan, Wu Nan,
Peng Liping, Wang Shizhen, Jiang Wen, Liu Wenchao, Kong Lu, 牟钰, Xu Fengxia, Wang Haikuan, Li Bifang, Yang Jun, Li Yi, Liu Yuxiong, Yang Guowu.
Determination of metallothionein content in biological samples
1 Scope
This standard specifies the liquid chromatography-tandem mass of rabbit-derived metallothionein type I and type II (MT-I and MT-II) in biological samples.
Spectral method.
This standard applies to the determination of the total content of rabbit metallothionein type I and type II in biological samples such as biological agents or biological extracts.
The detection limit of this method is 6.2 mg/kg.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 terms and definitions, abbreviations
3.1 Terms and definitions
The following terms and definitions apply to this document.
3.1.1
Characteristic peptide specificpolypeptide
The only polypeptide found in the trypsin hydrolysate of the target protein whose amino acid sequence has a specificity.
3.1.2
Metallothionein metalothionein
A class of low molecular weight proteins that are cysteine-rich and have a high ability to bind metals.
3.1.3
Metallothionein type I and type II metalothioneinIandII
Widely found in the internal organs of most mammals, especially liver and kidney cells, and participate in the regulation of a class of eggs
White matter.
3.2 Abbreviations
The following abbreviations apply to this document.
DTT. Dithiothreitol
IAA. 2-Iodoacetamide
MT. metallothionein (Metalothionein)
4 Principle
The sample was digested by trypsin to produce a characteristic peptide, and the content of the characteristic peptide in the sample was determined by liquid chromatography-tandem mass spectrometry.
The relative molecular mass ratio of the average molecular mass of MT-I and MT-II to the characteristic peptide was 3.16, and the MT-I in the sample was calculated.
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