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GB/T 37500-2019 English PDF

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GB/T 37500-2019: Determination of plant growth regulators in fertilizer - High performance liquid chromatography
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PDF similar to GB/T 37500-2019


Standard similar to GB/T 37500-2019

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Basic data

Standard ID GB/T 37500-2019 (GB/T37500-2019)
Description (Translated English) Determination of plant growth regulators in fertilizer - High performance liquid chromatography
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard G20
Classification of International Standard 65.080
Word Count Estimation 12,199
Date of Issue 2019-06-04
Date of Implementation 2020-01-01
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 37500-2019: Determination of plant growth regulators in fertilizer - High performance liquid chromatography


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Determination of plant growth regulators in fertilizer - High performance liquid chromatography ICS 65.080 G20 National Standards of People's Republic of China Determination of Plant Growth Regulators in Fertilizers High performance liquid chromatography 2019-06-04 released 2020-01-01 Implementation State Administration for Market Regulation Issued by China National Standardization Administration

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the China Petroleum and Chemical Industry Federation. This standard is under the jurisdiction of the National Standardization Technical Committee for Fertilizers and Soil Conditioners (SAC/TC105). Drafting organizations of this standard. Shanghai Research Institute of Chemical Industry Co., Ltd., Shandong Product Quality Inspection and Research Institute, Heilongjiang Quality Supervision and Inspection Research Institute, Yunnan Provincial Chemical Product Quality Supervision and Inspection Station, Sichuan Guoguang Agrochemical Co., Ltd., Shandong Nongda Fertilizer Technology Co., Ltd., Shanghai Hai Chemical Institute Testing Co., Ltd. The main drafters of this standard. Su Benyu, Zhang Juan, Duan Lulu, Liu Yufeng, Ding Fangjun, Wu Pandao, Wei Houdao, Mao Hongxiang, Zhu Hairong, Liu Wei, Wu Fan, Yao Sha. Determination of Plant Growth Regulators in Fertilizers High performance liquid chromatography Warning---Personnel using this standard should have practical experience in formal laboratory work. This standard does not point out all possible safety issues question. The user is responsible for taking appropriate safety and health measures and ensuring compliance with the conditions stipulated by relevant national laws and regulations.

1 Scope

This standard specifies the method for determining the content of plant growth regulators in fertilizers by high performance liquid chromatography. This standard applies to sodium nitrophenolate and 2,4-dichlorophenoxyacetic acid in water-soluble fertilizers, compound fertilizers, compound fertilizers, mixed fertilizers and other fertilizers (Referred to as 2,4-D), determination of abscisic acid, naphthalene acetic acid, forchlorfenuron, uniconazole, indole-3-acetic acid, indole butyric acid. The method detection limit and quantification limit of each target in this standard are. sodium nitrophenolate 3mg/kg and 10mg/kg; 2,4-D 5mg/kg and 10mg/kg; abscisic acid, naphthaleneacetic acid, forchlorfenuron, uniconazole 3mg/kg and 10mg/kg; indole-3-acetic acid, indole butyric acid 5mg/kg and 10mg/kg.

2 Normative references

The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Method summary

The sample is ultrasonically extracted with methanol, and under selected working conditions, with a high performance liquid chromatograph (equipped with a diode array detector) Determination is qualitative by retention time and quantitative by external standard method.

4 Reagents and materials

4.1 Unless otherwise stated, only use reagents confirmed to be analytically pure and primary water in GB/T 6682 in the analysis. 4.2 Methanol. chromatographically pure. 4.3 Acetonitrile. chromatographically pure. 4.4 Formic acid. chromatographically pure. 4.5 Ammonium acetate. chromatographically pure. 4.6 Glacial acetic acid. pure superior grade. 4.7 Formic acid solution (1 1000). Pipette 1.0mL formic acid (4.4) and 1000mL water and mix. 4.8 Ammonium acetate solution (0.77g/L). Weigh 0.77g of ammonium acetate (4.5), dissolve in water and dilute to 1000mL. 4.9 Glacial acetic acid solution (150). Pipette 20.0mL glacial acetic acid (4.6) and 1000mL water and mix. 4.10 Sodium 2-nitrophenolate, sodium 4-nitrophenolate, sodium 5-nitroo-methoxyphenolate, 2,4-dichlorophenoxyacetic acid (2,4-D), abscisic acid, naphthalene ethyl Acid, forchlorfenuron, uniconazole, indole-3-acetic acid, indole butyric acid standard. known mass fraction, w≥98.0%, for information see the table in Appendix A A.1. 4.11 Standard stock solution of sodium nitrophenolate. weigh an appropriate amount of sodium 2-nitrophenolate, sodium 4-nitrophenolate, and sodium 5-nitroo-methoxyphenol standard

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