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GB/T 37115-2018: Protocol of diagnosis for infection with abalone herpes virus
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 37115-2018 | English | 279 |
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Protocol of diagnosis for infection with abalone herpes virus
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GB/T 37115-2018
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Basic data | Standard ID | GB/T 37115-2018 (GB/T37115-2018) | | Description (Translated English) | Protocol of diagnosis for infection with abalone herpes virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 65.020.30 | | Word Count Estimation | 14,170 | | Date of Issue | 2018-12-28 | | Date of Implementation | 2019-07-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 37115-2018: Protocol of diagnosis for infection with abalone herpes virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of diagnosis for infection with abalone herpersvirus
ICS 65.020.30
B41
National Standards of People's Republic of China
Diagnosis of alopecia virus disease
Published on.2018-12-28
2019-07-01 implementation
State market supervision and administration
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. Publication of this document
The organization does not assume responsibility for identifying these patents.
This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic
This standard is under the jurisdiction of the National Aquaculture Standardization Technical Committee (SAC/TC156).
This standard was drafted. Shenzhen Institute of Inspection and Quarantine, National Aquatic Technology Extension Station, Shenzhen Customs of the People's Republic of China.
The main drafters of this standard. Wang Jinjin, Li Qing, Yu Weizhong, Liu Wei, Liu Ying, Shi Xiujie, Zheng Xiaocong, He Junqiang, Yu Li, Jia Peng, Lan Wensheng.
Diagnosis of alopecia virus disease
1 Scope
This standard gives the clinical symptoms of alopecia virus disease, specifies the histopathological detection method of alopecia virus disease, real-time fluorescence polymerization
Enzyme chain reaction detection method, polymerase chain reaction detection method.
This standard applies to the epidemiological investigation, monitoring, diagnosis and quarantine of alopecia virus disease.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 18088 entry and exit animal quarantine sampling
SC/T 7103 Technical specification for quarantine sampling of aquatic animals
3 Abbreviations
The following abbreviations apply to this document.
AbHV. Abalone Herpesvirus
CTAB. CetyltrimethylAmmonium Bromide
Ct value. Cycle threshold (Cyclethreshold)
EB. Ethidium Bromide
EDTA. Ethylene Diamine Tetraacetic Acid
PCR. Polymerase Chain Reaction (PolymeraseChainReaction)
qPCR. real-time fluorescent polymerase chain reaction (quantitativereal-timePolymeraseChainReaction)
4 reagents and materials
4.1 Unless otherwise stated, the biochemical reagents used in this standard are analytically pure reagents.
4.2 If not specified, the test water should meet the specifications of GB/T 6682 water.
4.3 Taq enzyme and 10 times Taq enzyme concentration buffer; Taq enzyme concentration is 5U/μL, stored at -20 ° C, to avoid repeated freezing and thawing.
4.4 dNTPs. containing 10 cmoL/L of dCTP, dGTP, dATP and dTTP.
4.5 qPCR primers and probes.
AbHVORF66F1. 5'-CCCGGACACCAGTAAGAAC-3'
AbHVORF66R1. 5'-AGGCTGCTATGCGTATGA-3'
Probe (AbHV66Prb1). 5'-6FAM-TGGCCGTCGAGATGTCCATG-TAMRA-3'
A 146 bp fragment of the ORF66 gene was amplified for fluorescent PCR. (See Appendix A)
AbHVORF77F1. 5'-AACCACTTGTTCGGGTTCT-3'
AbHVORF77R1. 5'-AGGGTGATTAATGCGGAGT-3'
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