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GB/T 36204-2018: Determination of gibberellic acid in fertilizer -- High performance liquid chromatography-mass spectrometry method
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 36204-2018 | English | 159 |
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Determination of gibberellic acid in fertilizer -- High performance liquid chromatography-mass spectrometry method
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GB/T 36204-2018
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Basic data | Standard ID | GB/T 36204-2018 (GB/T36204-2018) | | Description (Translated English) | Determination of gibberellic acid in fertilizer -- High performance liquid chromatography-mass spectrometry method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | G20 | | Classification of International Standard | 65.080 | | Word Count Estimation | 8,879 | | Date of Issue | 2018-05-14 | | Date of Implementation | 2018-12-01 | | Regulation (derived from) | National Standards Announcement No. 6 of 2018 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 36204-2018: Determination of gibberellic acid in fertilizer -- High performance liquid chromatography-mass spectrometry method
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(Determination of gibberellic acid in fertilizers HPLC-MS method)
ICS 65.080
G20
National Standards of People's Republic of China
Determination of gibberellic acid content in fertilizer
High performance liquid chromatography-mass spectrometry
Published on.2018-05-14
2018-12-01 implementation
State market supervision and administration
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed by the China Petroleum and Chemical Industry Federation.
This standard is under the jurisdiction of the National Fertilizer and Soil Conditioning Standardization Technical Committee (SAC/TC105).
This standard was drafted. Shandong Provincial Product Quality Inspection Institute, Shanghai Chemical Research Institute Co., Ltd., Yunnan Province Chemical Product Quality Supervision
Supervisory Inspection Station, Guizhou Provincial Product Quality Supervision and Inspection Institute, Henan Chemical Industry Research Institute Co., Ltd., Heilongjiang Provincial Quality Supervision and Inspection Institute, Pro
Handan City Product Quality Supervision and Inspection Institute, Shandong Agricultural University Fertilizer Technology Co., Ltd.
The main drafters of this standard. Zhang Juan, Duan Lulu, Gui Suping, Su Benyu, Chen Lei, Yan Fulong, Zhong Hongbo, Dou Xingxia, Zhu Hairong, Ding Fangjun,
Chen Meng, Du Xiuming, Hong Liling.
Determination of gibberellic acid content in fertilizer
High performance liquid chromatography-mass spectrometry
1 Scope
This standard specifies the determination of gibberellic acid in fertilizers by high performance liquid chromatography-triple quadrupole tandem mass spectrometry.
This standard is applicable to the determination of gibberellic acid in fertilizers such as water-soluble fertilizers, compound fertilizers (composite fertilizers) and blended fertilizers.
The detection limit of gibberellic acid in the method is 0.1 mg/kg, and the limit of quantification is 0.5 mg/kg.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Method summary
The sample was ultrasonically extracted with formic acid-methanol solution and formic acid solution, using formic acid-methanol solution and formic acid solution as mobile phase, using C18 as
The stainless steel column of the packing was measured by high performance liquid chromatography-triple quadrupole tandem mass spectrometry and quantified by external standard method.
4 reagents and materials
4.1 General Provisions. The reagents used in this standard refer to analytically pure reagents when no other requirements are indicated. Used in this standard
Water, if not specified, shall comply with the first grade water specification in GB/T 6682.
4.2 Methanol. chromatographically pure.
4.3 Formic acid. chromatographically pure.
4.4 Formic acid-methanol solution. Accurately transfer 1.0 mL of formic acid (4.3) and dilute to 1 L with methanol (4.2).
4.5 Formic acid solution. Accurately transfer 1.0 mL of formic acid (4.3) and dilute to 1 L with water.
4.6 Initial mobile phase. Formic acid-methanol solution (4.4) and formic acid solution (4.5) are mixed uniformly in a ratio of 1.9, and are now ready for use.
4.7 Gibberelic acid (CAS No. 77-06-5). Molecular formula. C19H22O6. Relative molecular mass. 346.38.
The purity is ≥99.0%.
4.8 gibberellic acid standard stock solution. accurately weigh gibberellic acid standard (4.7) 0.1g (accurate 0.1mg), placed in a 100mL volumetric flask,
Dissolve with methanol (4.2) and dilute to the mark. The concentration of the solution is 1 mg/mL. Store in the dark at 0 °C~2 °C in the dark. The effective period is 3 months.
4.9 gibberellic acid standard working solution. dilute the gibberellic acid standard stock solution (4.8) with methanol (4.2) to a working solution of 1 μg/mL, now
For distribution.
5 Instruments and equipment
5.1 High Performance Liquid Chromatography - Triple Quadrupole Mass Spectrometer. equipped with an electrospray ionization source (ESI).
5.2 Vortex mixer.
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