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GB/T 35939-2018 English PDF

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GB/T 35939-2018: Indirect enzyme linked immunosorbent assay for detecting the antibody against encephalomyocarditis virus
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GB/T 35939-2018English279 Add to Cart 3 days [Need to translate] Indirect enzyme linked immunosorbent assay for detecting the antibody against encephalomyocarditis virus Valid GB/T 35939-2018

PDF similar to GB/T 35939-2018


Standard similar to GB/T 35939-2018

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Basic data

Standard ID GB/T 35939-2018 (GB/T35939-2018)
Description (Translated English) Indirect enzyme linked immunosorbent assay for detecting the antibody against encephalomyocarditis virus
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.220
Word Count Estimation 14,189
Date of Issue 2018-02-06
Date of Implementation 2018-09-01
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 35939-2018: Indirect enzyme linked immunosorbent assay for detecting the antibody against encephalomyocarditis virus


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Indirect enzyme linked immunosorbent assay for detecting the antibody against encephalomyocarditis virus ICS 11.220 B41 National Standards of People's Republic of China Indirect ELISA for detection of encephalomyocarditis virus 2018-02-06 released 2018-09-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Animal Husbandry Standardization Technical Committee (SAC/TC181) centralized. This standard was drafted. Nanjing Agricultural University, China Animal Health and Epidemiology Center. Drafters of this standard. Bai Juan, Wang Yan, Jiang Ping, Jiang Kangfu. Indirect ELISA for detection of encephalomyocarditis virus

1 Scope

This standard specifies the encephalomyocarditis virus indirect ELISA detection method. This standard applies to the detection of porcine encephalomyocarditis virus antibody, serum encephalomyocarditis epidemiological investigation and laboratory diagnosis. Note. For a brief description of encephalomyocarditis epidemics, see Appendix A.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB 19489 laboratory biosafety common requirements NY/T 541 Technical Specification for Collection, Storage and Transportation of Veterinary Diagnostic Samples SN/T 2984 inspection and quarantine animal pathogenic microorganisms in biosafety requirements of the experimental activities

3 Abbreviations

The following abbreviations apply to this document. BSA. Bovine serum albumin ELISA. Enzyme-linked immunosorbent assay EMCV. Encephalomyocarditis virus HRP-SPA. Horseradishperoxidaselabeled staphylococcal horseradish peroxidase-labeled staphylococcal protein proteinA TMB. Tetramethylbenzidine VP1. Viral protein 1 4 instruments, materials and reagents 4.1 Test equipment 4.1.1 enzyme-linked immunosorbent assay. 4.1.2 high-speed desktop refrigerated centrifuge. 4.1.3 Micropipettes. Specifications 0.5μL ~ 10μL, 20μL ~.200μL, 100μL ~ 1000μL. 4.1.4 12 adjustable micropipette. Specifications 10μL ~ 100μL. 4.2 Test Materials 4.2.1 96-well enzyme-labeled reaction plate. 4.2.2 Syringe (5 mL).

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