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GB/T 35911-2018: Real-time PCR method for detection of pseudorabies virus
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 35911-2018 | English | 199 |
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Real-time PCR method for detection of pseudorabies virus
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GB/T 35911-2018
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Basic data | Standard ID | GB/T 35911-2018 (GB/T35911-2018) | | Description (Translated English) | Real-time PCR method for detection of pseudorabies virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 10,136 | | Date of Issue | 2018-02-06 | | Date of Implementation | 2018-09-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 35911-2018: Real-time PCR method for detection of pseudorabies virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Real-time PCR method for detection of pseudorabies virus
ICS 11.220
B41
National Standards of People's Republic of China
Pseudorabies virus fluorescent PCR detection method
Published on.2018-02-06
2018-09-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Administration issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed by the Ministry of Agriculture of the People's Republic
This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181).
This standard was drafted. Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shanghai Veterinary Research Institute of Chinese Academy of Agricultural Sciences, China
Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, China Academy of Inspection and Quarantine, Hunan Shengxiang Biotechnology Co., Ltd.
The main drafters of this standard. Zhang Qiang, Tong Guangzhi, Li Jian, Xiong Wei, Zhao Heping, Li Shuqing, Wang Yan, Li Guoxin, Yang Zhongping, Hua Qunyi, Lin Yingxi,
Wang Qiaoquan, Cai Kaimei, Yu Zhengjun, Wu Shaoqiang, Lin Xiangmei.
Pseudorabies virus fluorescent PCR detection method
1 Scope
This standard specifies the method for the detection of pseudorabies virus fluorescent PCR.
This standard applies to the detection of pseudorabies virus nucleic acid.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 25172 Pig normal temperature semen production and preservation technical specifications
3 Abbreviations
The following abbreviations apply to this document.
Fluorescent PCR fluorescent polymerase chain reaction (real-time polymerase chain reaction)
Ct value Cycle number (cyclethreshold) experienced when the amount of fluorescent signal in each reaction tube reaches a set threshold
DNA deoxyribonucleic acid (deoxyribonucleic acid)
Taq enzyme Taq DNA polymerase (TaqDNApolymerase)
TE buffer Tris-EDTA buffer (Tris-EDTAbuffer)
PRV pseudorabies virus (pseudorabiesvirus)
4 instruments
4.1 Fluorescence PCR detector.
4.2 High-speed desktop refrigerated centrifuge. can control temperature to 4 ° C, centrifugal speed can reach 12000r/min or more.
4.3 Tissue grinder or mortar.
4.4 Ordinary refrigerator. 2 ° C ~ 8 ° C.
4.5 Ordinary freezer. below -20 °C.
4.6 Ultra-low temperature refrigerator. can control temperature below -70 °C.
4.7 Micropipette. 0.2μL~2μL, 1μL~10μL, 10μL~100μL, 20μL~200μL, 100μL~1000μL, and
Comes with a tip that matches the pipette.
4.8 Autoclave.
5 supplies
5.1 1.5 mL centrifuge tube.
5.2 0.2mL PCR thin wall tube or eight tube.
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