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GB/T 35901-2018: Real-time PCR method for detection of porcine circovirus type 2
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 35901-2018 | English | 199 |
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Real-time PCR method for detection of porcine circovirus type 2
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GB/T 35901-2018
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Basic data | Standard ID | GB/T 35901-2018 (GB/T35901-2018) | | Description (Translated English) | Real-time PCR method for detection of porcine circovirus type 2 | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 10,117 | | Date of Issue | 2018-02-06 | | Date of Implementation | 2018-09-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 35901-2018: Real-time PCR method for detection of porcine circovirus type 2---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Real-time PCR method for detection of porcine circovirus type 2
ICS 11.220
B41
National Standards of People's Republic of China
Porcine circovirus type 2 fluorescent PCR detection method
2018-02-06 released
2018-09-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Animal Husbandry Standardization Technical Committee (SAC/TC181) centralized.
This standard was drafted. Shanghai Entry-Exit Inspection and Quarantine of the People's Republic of China, Shanghai Institute of Veterinary Medicine, Chinese Academy of Agricultural Sciences, China
Shenzhen Entry-Exit Inspection and Quarantine of People's Republic of China, China Inspection and Quarantine Science Institute, Hunan Shengxiang Biological Technology Co., Ltd.
The main drafters of this standard. Zhang Qiang, Li Jian, Xiong Wei, Tong Guangzhi, Zhao Heping, Hua Qunyi, Li Shuqing, Yang Zhongping, Li Guoxin, Wang Qiaoquan,
Wang Yan, Lin Yingzheng, Cai open sister, Yu Zhengjun, Wu Shaoqiang, Lin Xiangmei.
Porcine circovirus type 2 fluorescent PCR detection method
1 Scope
This standard specifies the operation of porcine circovirus type 2 fluorescent PCR test.
This standard applies to porcine circovirus type 2 nucleic acid testing.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
GB/T 25172 pig sperm production and preservation of technical specifications
3 Abbreviations
The following abbreviations apply to this document.
Fluorescent PCR. Fluorescent polymerase chain reaction (real-time polymerase chain reaction)
Ct value. the number of cycles in each reaction tube fluorescent signal reaches the set threshold value (cyclethreshold)
DNA. deoxyribonucleic acid
Taq enzyme. Taq DNA polymerase (Taq DNA polymerase)
TE buffer. Tris-EDTA buffer (Tris-EDTA buffer)
PCV. porcine circovirus
4 equipment
4.1 Fluorescent PCR detector.
4.2 high-speed desktop refrigerated centrifuge. temperature can be controlled to 4 ℃, centrifuge speed up to 12000r/min above.
4.3 Ordinary refrigerator. 2 ℃ ~ 8 ℃.
4.4 Ordinary freezer. -20 ℃ below.
4.5 ultra-low temperature refrigerator. temperature control can be -70 ℃ below.
4.6 Micropipettor. 0.2μL ~ 2μL, 1μL ~ 10μL, 10μL ~ 100μL, 20μL ~.200μL, 100μL ~ 1000μL and
Equipped with tips that match the pipette.
4.7 autoclave.
5 supplies
5.1 1.5mL centrifuge tube.
5.2 0.2mLPCR thin-walled tube or octagonal tube.
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