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GB/T 35909-2018: Detection of mycoplasma hyopneumoniae using PCR method
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Detection of mycoplasma hyopneumoniae using PCR method
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GB/T 35909-2018
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Basic data | Standard ID | GB/T 35909-2018 (GB/T35909-2018) | | Description (Translated English) | Detection of mycoplasma hyopneumoniae using PCR method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 10,112 | | Date of Issue | 2018-02-06 | | Date of Implementation | 2018-09-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 35909-2018: Detection of mycoplasma hyopneumoniae using PCR method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of mycoplasma hyopneumoniae using PCR method
ICS 11.220
B41
National Standards of People's Republic of China
Mycoplasma hyopneumoniae PCR detection method
2018-02-06 released
2018-09-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Animal Husbandry Standardization Technical Committee (SAC/TC181) centralized.
This standard was drafted. Jiangsu Academy of Agricultural Sciences.
Drafters of this standard. Shao Guoqing, Feng Zhixin, Xiong Qi Yan, Liu Maojun, Zhang Lei, Wang Haiyan, Wu Yuzi, Wei Yanna, Liu Beibei,
Wang Jia, Wang Li.
Mycoplasma hyopneumoniae PCR detection method
1 Scope
This standard specifies the method of PCR detection of Mycoplasma hyopneumoniae.
This standard applies to laboratory clinical samples of pigs (swine nasal swabs, lung and bronchoalveolar lavage fluid), bacterial culture and animal production
Rapid detection of Mycoplasma hyopneumoniae nucleic acid in
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
GB 19489 laboratory biosafety common requirements
GB/T 27401 Laboratory Quality Control Animal Quarantine
Diseases and disease harmless animal handling technical specifications Nongyifa [2017] No. 25
Veterinary Laboratory Biosafety Regulations (People's Republic of China Ministry of Agriculture Bulletin No. 302)
3 Terms and definitions and abbreviations
3.1 Terms and definitions
The following terms and definitions apply to this document.
3.1.1
Mycoplasma hyopneumoniae; MHP
Mycoplasma Mycoplasma is a member of the Mycoplasma pneumoniae (MPS) major pathogens.
Note. Mycoplasma hominis also known as swine asthma, is a chronic infection of the respiratory tract, causing serious damage to the pig industry.
3.2 Abbreviations
The following abbreviations apply to this document.
PCR polymerase chain reaction (polymerasechainreaction)
PBS phosphate buffer (phosphate-bufferedsalinebuffer)
TAE TAE electrophoresis buffer (tris-acetate-ethylenediaminetetraacetic acid buffer)
TBE TBE electrophoresis buffer (tris-borate-ethylenediaminetetraacetic acid buffer)
Taq polymerase Taq DNA polymerase (Taq DNA polymerase)
dNTPs deoxyribonucleosidetriphosphates
DNA deoxyribonucleic acid
CCU color change unit (colorchangeunit)
MPS Mycoplasma pneumonia of swine
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