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GB/T 35900.1-2018: Animal influenza detection -- Part 1: Method of real-time RT-PCR for the detection of H1 subtype influenza virus
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| GB/T 35900.1-2018 | English | 199 |
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Animal influenza detection -- Part 1: Method of real-time RT-PCR for the detection of H1 subtype influenza virus
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GB/T 35900.1-2018
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Basic data | Standard ID | GB/T 35900.1-2018 (GB/T35900.1-2018) | | Description (Translated English) | Animal influenza detection -- Part 1: Method of real-time RT-PCR for the detection of H1 subtype influenza virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 10,122 | | Date of Issue | 2018-02-06 | | Date of Implementation | 2018-09-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 35900.1-2018: Animal influenza detection -- Part 1: Method of real-time RT-PCR for the detection of H1 subtype influenza virus
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Animal influenza detection-Part 1. Method of real-time RT-PCR for the detection of H1 subtype influenza virus
ICS 11.220
B41
National Standards of People's Republic of China
Animal flu testing
Part 1. Fluorescence of H1 subtype influenza virus nucleic acids
RT-PCR detection method
Animalinfluenzadetection-Part 1.Method of real-time RT-PCRforthe
Published on.2018-02-06
2018-09-01 Implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Administration released
Foreword
GB/T 35900 Animal Influenza Test is currently divided into 3 parts.
--- Part 1. Fluorescent RT-PCR detection method for H1 subtype influenza virus nucleic acid;
--- Part 2. Detection of nucleic acid fluorescence of H3 subtype influenza virus by RT-PCR;
--- Part 3. Double-fluorescence RT-PCR detection method for H1 and H3 subtype influenza virus nucleic acids.
This part is part 1 of GB/T 35900.
This section was drafted in accordance with the rules given in GB/T 1.1-2009.
This part is proposed by the Ministry of Agriculture
This part is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181).
This section drafted by. People's Republic of China Beijing Entry-Exit Inspection and Quarantine Bureau, China Agricultural University.
The main drafters of this section are. Qiao Caixia, Gu Qiang, Gao Zhiqiang, Liu Huan, Pu Jing, Zhang Hexiao, Liu Jinhua, Sun Honglei, Zhang Wei, and Zhang Lifeng.
Introduction
The issuing authority of this document draws attention to the fact that, when the statement is in compliance with this document, this document may refer to 4.1.8 and “Detection of H1 and H3 subtypes.
The use of patents related to nucleotide sequences and kits of influenza viruses.
The issuing agency of this document has no position on the authenticity, validity and scope of the patent.
The patent holder has assured the issuing agency of this document that he is willing to apply to any applicant under reasonable and non-discriminatory terms and conditions.
Negotiate on licensing of patents. The patent holder’s statement has been filed with the issuing agency of this document. Related information can be passed below
Contact information is obtained.
Patent holder. Beijing Entry-Exit Inspection and Quarantine Bureau, People's Republic of China.
Address. 6 Tianshuiyuan Street, Chaoyang District, Beijing.
Please note that in addition to the above patents, some of the contents of this document may still involve patents. The issuing agency of this document does not undertake to identify these
Lee's responsibility.
Animal flu testing
Part 1. Fluorescence of H1 subtype influenza virus nucleic acids
RT-PCR detection method
1 Scope
This part of GB/T 35900 specifies a fluorescent RT-PCR method for the detection of H1 subtype influenza virus nucleic acids.
This section applies to the detection of H1 subtype influenza virus nucleic acids in swine and poultry and their products.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB/T 6682 Analysis Laboratory Water Specifications and Test Methods
GB/T 19438.1-2004 Avian influenza virus universal fluorescence RT-PCR detection method
GB 19489 General Requirements for Laboratory Biosafety
3 Abbreviations
The following abbreviations apply to this document.
Fluorescence RT-PCR Real-time reverse transcription polymerase chainre- (real-time reverse transcript polymerase chainre-
Action)
Ct (or Cp) value The number of cycles experienced by the amount of fluorescence signal in each reaction tube reaching the set threshold (cyclethreshold, or
Crossingpoint)
cRNA complementRNA
DEPC diethylpyrocarbonate
FAM carboxyfluorescein
LNA locked nucleic acid (locked nucleic acid)
PBS phosphate buffer solution
RNA ribonucleicacid
TAMRA carboxytetramethylrhodamine
4 Reagents and materials
4.1 Reagents
4.1.1 General. Unless otherwise stated, all reagents used are of analytical grade. All liquid reagents used should be packed in RNase-free containers.
4.1.2 TRIzol. Store at 2°C to 25°C.
4.1.3 Chloroform. Precooling at 2°C to 8°C.
4.1.4 Isopropyl alcohol. Precooled at -20°C.
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