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GB/T 35908-2018 English PDF

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GB/T 35908-2018: Diagnostic techniques for chalkbrood of honeybees
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PDF similar to GB/T 35908-2018


Standard similar to GB/T 35908-2018

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Basic data

Standard ID GB/T 35908-2018 (GB/T35908-2018)
Description (Translated English) Diagnostic techniques for chalkbrood of honeybees
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.220
Word Count Estimation 18,144
Date of Issue 2018-02-06
Date of Implementation 2018-09-01
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 35908-2018: Diagnostic techniques for chalkbrood of honeybees

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Diagnostic techniques for chalkbrood of honey bees ICS 11.220 B41 National Standards of People's Republic of China Chalk disease diagnosis technology for honeybees Published on.2018-02-06 2018-09-01 Implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Administration released Directory Preface I 1 Scope 1 2 Normative references 1 3 Abbreviations 1 4 Reagents and Materials 1 4.1 Water 1 4.2 Fixative, Staining Solution and Medium 1 4.3 PCR Detection Method Reagent 1 4.4 Primer Sequences and Control Samples 1 5 Equipment 2 6 Clinical diagnosis 2 6.1 Epidemiology 2 6.2 Observations outside the box 2 6.3 Unpacking Check 2 6.4 result determination 2 7 Laboratory Diagnostics 2 7.1 Sample 2 7.2 Microscopy 3 7.3 Separation culture 3 7.4 PCR detection 4 8 Comprehensive judgment 5 Appendix A (Normative) Fixatives, Staining Solutions, and Media Formulations 6 Appendix B (Normative) PCR Test Method Reagent Formula 7 Appendix C (Informative Appendix) Death and Insect Pest Symptoms in Bee Colonies of Bees Appendix D (Informative Appendix) Sickness of the bee colonies in bee disease 10 Appendix E (Informative Appendix) Microscopic images of bee bacillus under light microscopy 11 Appendix F (Informative Appendix) Electrophoresis of the PCR product of bee bacillus 13 Appendix G (Informative Appendix) Reference Gene Sequence of PCR Assay of Bee beet fungus 14

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed by the Ministry of Agriculture of the People's This standard is under the jurisdiction of the National Animal Health Standardization Technical Committee (SAC/TC181). This standard was drafted by. Fujian Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China Bureau, Fujian Agriculture and Forestry University, Fujian Saifu Food Testing Institute Co., Ltd. The main drafters of this standard. Zheng Teng, Zhang Tiyin, Song Zhanqi, Huang Shaokang, Li Songyi, Zhang Zhideng, Chen Richun, Wang Wujun, Bai Quanyang, Lin Sujie. Chalk disease diagnosis technology for honeybees

1 Scope

This standard specifies the diagnostic techniques for the chalk and bee of honeybees. This standard applies to the diagnosis and pathogen detection of bee chalk.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB/T 6682 Analysis Laboratory Water Specifications and Test Methods GB 19489 General Requirements for Laboratory Biosafety

3 Abbreviations

The following abbreviations apply to this document. EB. Ethidium bromide PCR. Polymerase chain reaction SDS. Sodium dodecylsulfate

4 Reagents and materials

4.1 Water The water used in this standard shall meet the specifications of the first-grade water in GB/T 6682. 4.2 Fixatives, Staining Solutions, and Culture Media Lactic acid phenol fixative (Formula A.1 in Appendix A), Lactic Acid Cotton Blue Staining Solution (see Formula A.2), Pathogen Separation Medium (with See A.3). 4.3 PCR detection reagents Spermidine-SDS buffer (formula B.1 in Appendix B), saturated phenol, chloroform, 3 mol/L sodium acetate (pH 5.5) (formula see B.2), TE buffer (for formulation, see B.3), 0.5.times.TBE buffer (for formulation, see B.4), loading buffer (for formulation, see B.5), 1.5% agarose gel. (Formulation see B.6), TaqDNA polymerase (5 U/μL), 10×PCR buffer, dNTPMix (10 mmol/L). 4.4 Primer Sequences and Control Samples Upstream Primer (F). 5'-TGTCTGTGCGGCTAGGTG-3'; Downstream primer (R). 5'-GAWCACGACGCCGTCACT-3'; the amplification product was 442 bp. A positive control of the honeybee bee bacterium, provided or synthesized by a designated unit with a plasmid containing a positive fragment. For the negative control of the bee bacterium, the healthy larvae without clinical signs of chalkiness can be used as negative controls.

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