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GB/T 35890-2018 English PDF

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GB/T 35890-2018: Technical specification of high throughput sequencing data format
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PDF similar to GB/T 35890-2018


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Basic data

Standard ID GB/T 35890-2018 (GB/T35890-2018)
Description (Translated English) Technical specification of high throughput sequencing data format
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard A40
Classification of International Standard 07.080
Word Count Estimation 14,182
Date of Issue 2018-02-06
Date of Implementation 2018-09-01
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 35890-2018: Technical specification of high throughput sequencing data format

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Technical specification of high throughput sequencing data format ICS 07.080 A40 National Standards of People's Republic of China High throughput sequencing data format specification Published on.2018-02-06 2018-09-01 Implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Standardization Technical Committee for Biochemical Detection (SAC/TC387). This standard was drafted by Shenzhen Huada Gene Research Institute and China Institute of Metrology. The main drafters of this standard. Liang Xinming, Liu Xin, Jiang Hui, Du Jiating, Xie Qiang, Li Qianyi, Li Yiyi, and Wang Jing. High throughput sequencing data format specification

1 Scope

This standard specifies the sequence format for high-throughput sequencing data, including the format of the sequence description format and the overall format of high-throughput sequencing data. specification. This standard applies to the specification of biological DNA high throughput sequencing data sequence format.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB/T 30989 High Throughput Gene Sequencing Technical Regulations ISO /IEC 646 Information Technology ISO Information Exchange Seven-bit Codeword Set (Informationtechnology-ISO 7-bitcoded Charactersetforinformationinterchange)

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 High-throughput sequencing Sequencing of DNA with a sequence of hundreds of thousands to several millions of nucleic acid molecules and a short read length technology. Note. Rewrite GB/T 30989-2014, definition 3.1.9. 3.2 Sequencing reads Sequence fragments generated by high-throughput sequencing platforms containing base sequences and mass values. 3.3 Double ended sequencing paired-endsequencing The DNA template strand and the complementary strand were sequenced separately, and the sequencing technology for pairwise sequencing of the two strands was obtained. 3.4 Insert length insertsize In the double-end sequencing, the distance from the left end of the sequencing fragment sequenced from the template strand to the right end of the sequencing fragment of the complementary strand sequence. 3.5 Sequencing fragment identification code readersidentifier A unique string used to identify a piece of sequencing. 3.6 Base sequence A string that records the base sequence in the sequencing segment. Each base in the base sequence should use uppercase letters (A, T, C, G, and N) or Lowercase letters (a, t, c, g, and n), where the letters A and a represent adenine, the letters T and t represent thymine, and the letters C and c represent cytosine

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