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GB/T 35537-2017: Requirements of the high-throughput gene sequencing result evaluation
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Requirements of the high-throughput gene sequencing result evaluation
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GB/T 35537-2017
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Basic data | Standard ID | GB/T 35537-2017 (GB/T35537-2017) | | Description (Translated English) | Requirements of the high-throughput gene sequencing result evaluation | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A40 | | Classification of International Standard | 07.080 | | Word Count Estimation | 10,182 | | Date of Issue | 2017-12-29 | | Date of Implementation | 2018-07-01 | | Regulation (derived from) | National Standards Bulletin 2017 No. 32 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 35537-2017: Requirements of the high-throughput gene sequencing result evaluation---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Requirements of the high-throughput gene sequencing result evaluation
ICS 07.080
A40
National Standards of People's Republic of China
High-throughput gene sequencing results evaluation requirements
2017-12-29 Posted
2018-07-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed by the National Biochemical Test Standardization Technical Committee (SAC/TC387) and centralized.
This standard drafting unit. Shenzhen Huada Gene Research Institute, Guangdong Provincial Institute of Standardization, Guangdong Institute of product quality supervision and inspection, Shenzhen
Huada Gene Technology Co., Ltd., China Institute of Food and Drug Control.
The main drafters of this standard. Yang Huanming, Xu News, Jiang Hui, Zhang Wenwei, Xi Feng, Wang Juan, Tan Jiali, Xie Qiang, Du Jia Ting, Li Qian Yi, Dai Dai Yi,
Cheng Lei, Yu Jing, Hu Mi, Huang Jie, Song Kun, Zhang Juan.
High-throughput gene sequencing results evaluation requirements
1 Scope
This standard specifies the terminology and definitions involved in the evaluation of high-throughput gene sequencing results evaluation of high-throughput gene sequencing results evaluation
Indicators and judgments.
This standard applies to DNA sequencing based on non-single-molecule sequencing of ligase sequencing and polymerase sequencing of the results of evaluation.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 30989 high throughput gene sequencing technical specifications
3 Terms and definitions and abbreviations
3.1 Terms and definitions
GB/T 30989 defined and the following terms and definitions apply to this document.
3.1.1
High-throughput gene sequencing results high-throughputgenesequencingresult
The data produced by the sequencer is also known as the sequencing result.
3.1.2
The average length of sequencing averagereadlengthofsequencing
The average read length that a sequencing instrument can achieve in a single run.
Note. usually expressed in terms of bases.
3.1.3
Sequencing throughput sequencingthroughput
The number of sequences available to a sequencer in a single run.
Note. Usually expressed as a sequence number (Reads).
3.1.4
Base sequencing accuracy sequencingaccuracy
The reference sequence of the known sequence is sequenced, compared to the known sequence after recognition of the base, and the number of the correct base of the statistical alignment is accounted for by sequencing
The total number of bases obtained.
3.1.5
Sequencing by polymerase
Based on the principle of base complementation, gene sequencing method with polymerase involved.
3.1.6
Ligase sequencing
Based on the principle of base complementation, gene sequencing method with the involvement of ligase.
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