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GB/T 35271-2017: Detection and identification of Maize chlorotic dwarf virus
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Detection and identification of Maize chlorotic dwarf virus
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GB/T 35271-2017
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Basic data | Standard ID | GB/T 35271-2017 (GB/T35271-2017) | | Description (Translated English) | Detection and identification of Maize chlorotic dwarf virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 18,159 | | Date of Issue | 2017-12-29 | | Date of Implementation | 2018-07-01 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 35271-2017: Detection and identification of Maize chlorotic dwarf virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Maize chlorotic dwarf virus
ICS 65.020.01
B16
National Standards of People's Republic of China
Quarantine and identification methods of corn chlorotic dwarf virus
Published on.2017-12-29
2018-07-01 Implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. Publication of this document
The agency does not assume responsibility for identifying these patents.
This standard is proposed and managed by the National Commission for Standardization of Plant Quarantine (SAC/TC271).
This standard was drafted by the People's Republic of China. Shandong Entry-Exit Inspection and Quarantine Bureau, Institute of Oceanology, Chinese Academy of Sciences, Chinese People's Republican
Fujian Entry-Exit Inspection and Quarantine Bureau.
The main drafters of this standard. Wu Xinghai, Zheng Yuan, Deng Mingjun, Ji Ji, Su Zhiping, Zhang Yunxia, Feng Liping, Shen Jianguo.
Quarantine and identification methods of corn chlorotic dwarf virus
1 Scope
This standard specifies the quarantine and identification methods for maize chlorotic dwarf virus.
This standard applies to quarantine and identification of maize chlorotic dwarf virus on incoming and outgoing grain crops.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB/T 6682 Analysis Laboratory Water Specifications and Test Methods
SN/T 1840 Plant Virus Immunoelectron Microscopy Assay
SN/T 2122 Quarantine sampling of inbound and outbound plant and plant products
SN/T 2964 Plant Virus Detection Specification
3 Maize chlorotic dwarf virus basic information
Scientific name. Maizechloroticdwarfvirus.
Synonym. Ohiocornstuntagent.
Abbreviation. MCDV.
Taxonomic status. Associated with Secoviridae and Waikavirus.
See Appendix A for additional information on maize chlorotic dwarf virus.
4 Methodology
According to the biological, immunological and molecular biological characteristics of the virus, double-antibody sandwich enzyme-linked immunosorbent assay based on antigen-antibody reaction
Assay (DAS-ELISA), reverse transcriptase polymerase chain reaction (RT-PCR), real-time fluorescence RT-PCR, and immunoelectron microscopy (IEM)
Test identification.
5 Equipment and main reagents
5.1 Instruments and Equipment
Electronic Balance (Sense 0.001g), Ordinary Balance (Sense 0.1g), High Speed Refrigerated Centrifuge, Small Instantaneous Centrifuge, Normal Refrigerator, Ultra
Low-temperature refrigerator (-80 °C), vortex oscillator, autoclave, pH meter, clean bench, PCR machine, fluorescent PCR instrument, microwave oven, electrophoresis
Instrument, electrophoresis tank, gel analysis imaging system, enzyme-linked detector, transmission electron microscope.
5.2 Appliances
Adjustable pipettes (2.5μL, 10μL, 20μL, 100μL, 1000μL) and tips, centrifuge tubes, PCR tubes, and mortar.
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