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GB/T 34729-2017 English PDF

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GB/T 34729-2017: Blocking ELISA method to detect antibody against classical swine fever virus
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Basic data

Standard ID GB/T 34729-2017 (GB/T34729-2017)
Description (Translated English) Blocking ELISA method to detect antibody against classical swine fever virus
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.220
Word Count Estimation 10,173
Date of Issue 2017-11-01
Date of Implementation 2018-05-01
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 34729-2017: Blocking ELISA method to detect antibody against classical swine fever virus

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Blocking ELISA method to detect antibody against classical swine fever virus ICS 11.220 B41 National Standards of People's Republic of China Detection of classical swine fever virus blocking ELISA antibody Posted.2017-11-01 2018-05-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China China National Standardization Administration released

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Animal Husbandry Standardization Technical Committee (SAC/TC181) centralized. This standard was drafted by the China Veterinary Drug Administration. The main drafters of this standard. Wang Qin, Xu Lu, Fan Xue Zheng, Zhao Qizu, Zhu Yuan Yuan, Zou Xingqi, Ning Yi Bao.

Introduction

The issuing authority of this document draws attention to the fact that statements that conform to this document may relate to the expression and purification of E2 protein of classical swine fever virus Off the use of the patent. The issuing authority of this document has no position whatsoever about the truthfulness, validity and scope of this patent. The patentee has assured the issuing authority of this document that he is willing to work with any applicant on reasonable and non-discriminatory terms and conditions, Negotiate a patent license. The patent holder's statement has been filed with the issuing authority of this document. Related information is available through the following link Department obtained. Name of patent holder. China Veterinary Drug Administration Beijing Zhongguancun South Street on the 8th Please note that in addition to the above patents, some of the contents of this document may still involve patents. The issuing agencies of this document are not responsible for identifying these specializations Lee's responsibility. Detection of classical swine fever virus blocking ELISA antibody

1 Scope

This standard specifies the detection of swine fever virus antibody blocking ELISA method. This standard applies to swine fever antibody testing, but can not distinguish vaccine immunogens and wild-type antibodies.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. Veterinary Laboratories Biosafety Technical Management Specification Ministry of Agriculture Bulletin No. 302

3 Abbreviations

The following abbreviations apply to this document. CSFV Classical swine fever virus ELISA. Enzyme-Linked Immunosorbent Assay HRP. Horseradish Peroxidase OD. Optical Density (OpticalDensity)

4 reagent

4.1 Classical swine fever virus E2 protein. 4.2 Enzyme conjugates. See Appendix B. 4.3 standard positive serum. Swine fever vaccine immunization piglets prepared by fluorescence antibody virus neutralization test was positive. 4.4 Standard Negative Serum. The serum of piglets without maternal antibodies and non-immunized swine fever vaccine was negative by fluorescent antibody virus neutralization test. 4.5 Coating liquid. See C.1. 4.6 Phosphate buffer. See C.2. 4.7 Blocking fluid See C.3. 4.8 20 times concentrated lotion. See C.4. 4.9 Dilutions See C.5. 4.10 Substrate Solution A. See C.6. 4.11 Substrate B. See C.7. 4.11 Stop solution. See C.8. 4.12 Commercial kits. Can choose the same commercial kits.

5 Equipment and equipment

5.1 37 ℃ incubator.

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