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GB/T 34728-2017: PCR for Mycoplasma agalactiae
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 34728-2017 | English | 199 |
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PCR for Mycoplasma agalactiae
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GB/T 34728-2017
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Basic data | Standard ID | GB/T 34728-2017 (GB/T34728-2017) | | Description (Translated English) | PCR for Mycoplasma agalactiae�� | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 10,186 | | Date of Issue | 2017-11-01 | | Date of Implementation | 2018-05-01 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 34728-2017: PCR for Mycoplasma agalactiae---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
PCR for Mycoplasma agalactiae
ICS 11.220
B41
National Standards of People's Republic of China
Milk-free Mycoplasma PCR assay
PCR for Mycoplasmaagalactiae
Posted.2017-11-01
2018-05-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the National Animal Husbandry Standardization Technical Committee (SAC/TC181) centralized.
This standard was drafted. Lanzhou Veterinary Institute of Chinese Academy of Agricultural Sciences.
Drafters of this standard. Chu Yuefeng, Zhao Ping, Gao Pengcheng, Chen Shengli, He Ying, Zhi Zhong new.
Milk-free Mycoplasma PCR assay
1 Scope
This standard specifies the non-milk mycoplasma PCR detection method of technical requirements.
This standard applies to the detection of sheep (goats and sheep) clinical samples and isolation of non-mycoplasma nucleic acids in culture.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
3 Abbreviations
The following abbreviations apply to this document.
CA. Contagious Agalactia
Maga. Mycoplasma agalactiae
PCR. Polymerase Chain Reaction (Polymerase Chain Reaction)
TAE. Nucleic Acid Running Buffer (Tris-Aceticacid-EDTA)
CCU. Color change unit (Colorchangeunit)
Taq enzyme Taq DNA polymerase (Taq DNA Polymerase)
DNA. Deoxyribonucleic Acid
dNTPs. Deoxyribonucleoside Triphosphates
bp. BasePair
4 equipment
4.1 Instrument
High-speed refrigerated centrifuge, PCR amplification instrument, nucleic acid electrophoresis, horizontal electrophoresis tank, constant temperature water bath, ordinary refrigerator, low temperature refrigerator (-70 ℃),
Gel imaging system, autoclave, tissue homogenization equipment.
4.2 equipment
(0.5 μL to 10 μL, 2 μL to 20 μL, 20 μL to.200 μL, 100 μL to 1000 μL), 0.22 μm sterile filter
Sterile cotton swabs, sterile sampling containers.
5 reagent
5.1 2 × PCR reaction premix (containing Taq enzyme, dNTPs and loading buffer).
5.2 DL2000 DNA molecular weight standards.
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