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GB/T 34223-2017: Determination of the purity of ribonuclease and desoxyribonuclease
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| GB/T 34223-2017 | English | 119 |
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Determination of the purity of ribonuclease and desoxyribonuclease
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GB/T 34223-2017
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Basic data | Standard ID | GB/T 34223-2017 (GB/T34223-2017) | | Description (Translated English) | Determination of the purity of ribonuclease and desoxyribonuclease | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A21 | | Word Count Estimation | 6,628 | | Date of Issue | 2017-09-07 | | Date of Implementation | 2018-04-01 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 34223-2017: Determination of the purity of ribonuclease and desoxyribonuclease---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of the purity of ribonuclease and desoxyribonuclease
ICS 07.080
A21
National Standards of People's Republic of China
Ribonuclease and DNase Purity
Detection method
2017-09-07 Posted
2018-04-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard proposed by the China National Institute of Standardization and centralized.
This standard was drafted unit. China National Institute of Standardization, Georgia Gelin Biotechnology Co., Ltd., China Biotechnology Development Center, Zhejiang
Business University, Agricultural University of Hebei, Hefei University of Technology, Exit Inspection and Quarantine of Hebei Province, Quanzhou Institute of Standardization, Beijing Food
Institute of Science, Hebei Food Inspection Institute, Dongying City standardized information, Henan University.
The main drafters of this standard. Ma Aijin, Chen Zhiyong, Su Yue, Wang Yanbo, Sun Records, Zheng Lei, Liu Daoliang, Lin Qingshan, Sun Yong, Yun Zhenyu,
Fu Linglin, Wu Qi, Zhao Lin, Zhou Wei, Liu Peng, Kang Arts, Zong Xuehua.
Ribonuclease and DNase Purity
Detection method
1 Scope
This standard specifies the principle of ribonuclease and deoxyribonuclease purity detection, equipment and apparatus, the main reagents, analysis steps
And result analysis.
This standard applies to biochemical reagents ribonuclease and deoxyribonuclease product production and testing.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
SN/T 3926 exports of milk, eggs, beans Determination of protein content in food Coomassie brilliant blue method
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
RNase ribonuclease; RNase
A nuclease that hydrolyzes a phosphodiester bond in ribonucleic acid (RNA) to form an oligonucleotide or a single nucleotide.
3.2
DNase deoxyribonuclease; DNase
Phosphodiester hydrolysis in deoxyribonucleic acid (DNA), generating oligonucleotides or mononucleotide nucleases.
4 principle
A certain concentration of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) can separate proteins with different molecular weights, the
Coomassie Brilliant Blue (CBB) staining, decolorization imaging, nuclease purity by calculation.
5 equipment and appliances
5.1 electrophoresis system.
5.2 Gel scanning device.
5.3 horizontal decolorization shaker, speed 45r/min.
5.4 Electronic balance. Accuracy of 0.0001g, 0.01g and 0.1g.
6 major reagents
The reagents used in this method are of analytical grade, the experimental water is GB/T 6882 provisions of the secondary water.
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