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GB/T 34222-2017: Determination of the activity of ribonuclease
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Determination of the activity of ribonuclease
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GB/T 34222-2017
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Basic data | Standard ID | GB/T 34222-2017 (GB/T34222-2017) | | Description (Translated English) | Determination of the activity of ribonuclease | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B20/39 | | Classification of International Standard | 07.080 | | Word Count Estimation | 6,695 | | Date of Issue | 2017-09-07 | | Date of Implementation | 2018-04-01 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 34222-2017: Determination of the activity of ribonuclease---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of the activity of ribonuclease
ICS 07.080
B20/39
National Standards of People's Republic of China
Ribonuclease activity assay
2017-09-07 Posted
2018-04-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard proposed by the China National Institute of Standardization and centralized.
This standard was drafted unit. China National Institute of Standardization, Georgia Gelin Biotechnology Co., Ltd., Fujian SAFU Food Testing Institute have
Limited, Zhejiang Technology and Business University, Agricultural University of Hebei, Hefei University of Technology, Exit Inspection and Quarantine of Hebei Province, Quanzhou Institute of Standardization,
Beijing Food Science Research Institute, Hebei Institute of Food Inspection, Dongying City standardized information, Henan University.
The main drafters of this standard. Ma Aijin, Chen Zhiyong, Huang Xiujuan, Wang Yanbo, Sun Ji Lu, Zheng Lei, Liu Daoliang, Lin Qingshan, Sun Yong, Yun Zhenyu,
Fu Linglin, Wu Qi, Zhao Lin, Zhou Wei, Liu Peng, Kang Arts, Zong Xuehua.
Ribonuclease activity assay
1 Scope
This standard specifies the principle of ribonuclease activity testing, equipment and apparatus, the main reagents, analysis steps and results analysis.
This standard applies to biochemical reagents ribonuclease activity test.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
RNase ribonuclease; RNase
A nuclease that hydrolyzes a phosphodiester bond in ribonucleic acid (RNA) to form an oligonucleotide or a single nucleotide.
3.2
Ribonuclease activity unit ribonucleaseactivityunit
1.5 mL of yeast RNA at a concentration of 0.15 mg/mL was used as a substrate, and the reaction was allowed to proceed within 1 min at 50 ° C and pH 5.0
The amount of enzyme required to increase the absorbance by 0.001 at 260 nm.
Note. An enzyme activity unit to U said.
4 principle
Ribonuclease catalyzes the decomposition of ribonucleic acid, and the degradation product has a characteristic absorption peak at a wavelength of 260 nm. The ribose
Nuclease enzyme activity.
5 equipment and appliances
5.1 constant temperature water bath. Accuracy of 0.1 ℃.
5.2 UV - visible spectrophotometer with an accuracy of 0.001.
5.3 pH meter. Accuracy of 0.01.
5.4 1cm quartz cuvette.
5.5 electronic balance with accuracy of 0.0001g, 0.01g or 0.1g.
6 Reagents
Reagents used in this method are of analytical grade, except for special instructions, the experimental water are secondary water GB/T 6882 regulations.
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