Search result: GB/T 34168-2017
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Test method of gold and silver nanoparticle materials biological effect by transmission electron microscope
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GB/T 34168-2017
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| Standard ID | GB/T 34168-2017 (GB/T34168-2017) | | Description (Translated English) | Test method of gold and silver nanoparticle materials biological effect by transmission electron microscope | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | G04 | | Classification of International Standard | 71.040.40 | | Word Count Estimation | 14,170 | | Date of Issue | 2017-09-07 | | Date of Implementation | 2018-08-01 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China, China National Standardization Administration Committee |
GB/T 34168-2017
Test method of gold and silver nanoparticle materials biological effect by transmission electron microscope
ICS 71.040.40
G04
National Standards of People's Republic of China
Biological effects of gold and silver nanoparticle materials
Transmission electron microscopy
2017-09-07 Posted
2018-08-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Directory
Foreword Ⅲ
Introduction IV
1 range 1
2 Terms and definitions 1
3 basic principles 1
4 equipment, reagents and environmental conditions 1
5 Sample Preparation 2
6 measurement methods 3
7 test report 4
Appendix A (informative) Preparation of glutaraldehyde fixative 5
Appendix B (Informative) Preparation of phosphate buffer 6
Appendix C (informative) osmium tetroxide fixed liquid preparation 7
Appendix D (informative) epoxy Epon812 embedding agent preparation 8
Reference 9
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard by the National Microbeam Analysis Standardization Technical Committee (SAC/TC38) and focal point.
This standard was drafted by the Chinese People's Liberation Army Second Military Medical University, National Nanoscience and Technology Center.
The main drafters of this standard. Yang Yong Ji, Tang Ying, Ge Guang Road, Wang Xiaoping.
introduction
It is unclear whether the widespread use of gold and silver nanoparticle materials can harm biological systems. The main cause of this situation
The reason is that one of the biological effects of gold and silver nanoparticle materials is not clear, including whether they can enter the organism, how to enter the organism,
Whether the object affects the function of the organism; the second is the lack of uniform technical and testing standards for the testing of gold and silver nanoparticle materials,
Really gold, the true size of silver nanoparticles; Third, gold, silver nanoparticles on the organism's safety effect test nor standard can be found, resulting in
Nano-material on the safety of organisms is not clear. Therefore, the establishment of these three aspects of the research system is nanomaterials in the life sciences research and should
With the key and accurate detection of gold and silver nanoparticles on the cell ultrastructure is the impact of gold and silver nanoparticle material biological effects
The important foundation.
Biological effects of gold and silver nanoparticle materials
Transmission electron microscopy
1 Scope
This standard specifies the use of transmission electron microscopy (hereinafter referred to as transmission electron microscopy) detection of gold, silver nanoparticles containing biological samples super
Microstructure technology and specifications.
The criteria specified in this standard are mainly applicable to the detection and analysis of the biological effects of transmission electron microscopy on gold and silver nanomaterials.
2 Terms and definitions
The following terms and definitions apply to this document.
2.1
Gold, silver nano-particles goldandsilvernanoparticle
Au, Ag particles less than 100 nm in size.
2.2
Gold, silver nanoparticles aggregates goldandsilvernanoparticlecoacervate
Au, Ag nanoparticles in a reunion state.
2.3
Biological effects biologicaleffect
The life process due to the intervention of nanomaterials physiological functions, biochemical functions and morphological changes.
2.4
Biological effects detection biologicaleffecttest
Biological effects of nanomaterials are examined and measured using experimental techniques in the fields of biology, physics, chemistry, toxicology and medicine.
2.5
Biopsy sample thinbiologicalsample
The biological thin sample refers to a biological sample prepared to be suitable for transmission electron microscope observation, and the thickness is usually below 100 nm.
3 basic principles
The physical and chemical properties of gold and silver nanoparticles make them in the state of aggregation in the normal state, and the size of the aggregated gold and silver nanoparticle material is not
All nanoscale, need to use physical or chemical method to disperse it into nano-scale particles. Dispersed gold, silver nanoparticles and biological
Body contact, to study its biological effects. Organisms that are in contact with gold and silver nanoparticles require the use of biological tissue or cell ultrastructure
Sample preparation method, made of biological thin samples, and then under the transmission electron microscope to detect gold and silver nanoparticles acting on the body after the trigger cells and
Changes in the ultrastructure of organelles to determine the biological effects of gold and silver nanoparticles.
4 equipment, reagents and environmental conditions
4.1 Equipment
4.1.1 Transmission electron microscope.
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