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GB/T 31809-2015 English PDF

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GB/T 31809-2015: Detection and identification of Pythium splendens Braun
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PDF similar to GB/T 31809-2015


Standard similar to GB/T 31809-2015

GB/T 43165   GB/T 40135   GB/T 19495.4   GB/T 31800   GB/T 31799   

Basic data

Standard ID GB/T 31809-2015 (GB/T31809-2015)
Description (Translated English) Detection and identification of Pythium splendens Braun
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 65.020.01
Word Count Estimation 15,138
Date of Issue 2015-07-03
Date of Implementation 2015-11-27
Regulation (derived from) National Standard Announcement 2015 No.22
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 31809-2015: Detection and identification of Pythium splendens Braun

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Pythium splendens Braun ICS 65.020.01 B16 National Standards of People's Republic of China Oil palm cataplexy quarantine and identification of bacteria Issued on. 2015-07-03 2015-11-27 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China Exit Inspection and Quarantine, Ningbo, Zhejiang, People's Republic of China Entry-Exit Inspection and Quarantine Bureau, Chinese Academy of Inspection and Quarantine. The main drafters. Zhang Huili, Zhang Jiancheng, Gu Jianfeng, Cheng Wei, Xu Ying, Chen Xianfeng, the military section of Victoria, Chenwu Jian, Wu Zhiyi, Du Hongzhong, Shan Wu products. Oil palm cataplexy quarantine and identification of bacteria

1 Scope

This standard specifies the oil palm cataplexy bacteria morphological and molecular biology quarantine and identification methods. This standard applies to quarantine and identification of damping-off pathogen host plants oil palm plants, cuttings and other plant materials and media.

2 Equipment

Microscope (with oil immersion lens), with the transmission of light stereo microscope (maximum magnification of not less than 50 ×), Autoclave, days Ping (a sense of volume 1/100g, 1/10000g), mortar, ice machines, water meter, Vortex, desktop centrifuge, spectrophotometer, high-speed refrigerated Centrifuges, vacuum drained instrument, low temperature refrigerators, refrigerator-freezers refrigerator, clean benches, conventional PCR, optical PCR reaction tube, pH meter, electricity Swimming apparatus, the horizontal electrophoresis tank, real-time PCR instrument, micropipette (0.5μL, 2μL, 10μL, 20μL, 100μL, 200μL, 1000μL), PCR reaction tube (0.2mL, 0.5mL), flasks, test tubes, petri dishes, slides, cover slips.

3 Reagents and culture medium

Unless otherwise specified, all reagents were analytical grade use. Sterile double distilled water, liquid nitrogen, proteinase K, sodium hydroxide, ammonium acetate, potassium chloride, Ethanol, ethidium bromide, chloroform, isopropanol, isoamyl alcohol, ampicillin, rifampicin, nystatin, benomyl, sodium chloride, CTAB, TaqDNA polymerase, dNTP mixture, 10 × PCR buffer, agarose, of DNA molecular weight standards, Tris/EDTA / SDS extraction buffer (TES), Tris/EDTA buffer (TE), Tris Borate EDTA buffer (the TBE) and the like. Medium and Slow Red liquid preparation see Appendix B.

4 quarantine and identification

4.1 Symptom Check Carefully check the roots, the roots of the symptoms is to soften brown rot; stem symptoms appear gray or brown-black water-soaked spots (see Appendix A). Suspicious plants and soil media propagating material samples should be further examined. 4.2 organizational separation Election wilting and browning the roots but not completely decaying plant material, with running tap water rinse, at the junction of Healthy roots cut 0.5cm × 0.5cm of the small piece of tissue, with a 1% sodium hypochlorite for surface disinfection 3min, sterile water three times, dry the surface sterilizing filter Water, and placed on a selective medium, 25 ℃ ~ 30 ℃ dark culture was observed daily until grow hyphae, hyphae picked transferred to the tip On the basis of the medium and purified for use. 4.3 soil trap Leaves of grasses (such as. crabgrass, foxtail, barnyard grass, etc.) high temperature autoclaving, cut into small pieces the size of the diameter of 1cm As bait. Take 5g ~ 20g soil sample was ground broken into 50mL sterile clean beaker, no thicker than 2cm, no added slowly Bacteria water over the soil surface 1cm ~ 2cm, gently with absorbent paper to remove surface film formation and organic residues floating, each beaker placed water trap Bait around 10. Allowed to stand overnight under stereo microscope observation, there will grow mycelium bait removed, washed with sterile water surface, sterile suction

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