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GB/T 31800-2015: Detection and identification of Plum pox virus
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Detection and identification of Plum pox virus
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GB/T 31800-2015
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Basic data | Standard ID | GB/T 31800-2015 (GB/T31800-2015) | | Description (Translated English) | Detection and identification of Plum pox virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 19,176 | | Date of Issue | 2015-07-03 | | Date of Implementation | 2015-11-27 | | Regulation (derived from) | National Standard Announcement 2015 No.22 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 31800-2015: Detection and identification of Plum pox virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Plum pox virus
ICS 65.020.01
B16
National Standards of People's Republic of China
Plum pox virus quarantine and identification methods
Issued on. 2015-07-03
2015-11-27 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
This standard was drafted. People's Republic of China, Ningbo Entry-Exit Inspection and Quarantine of Guangdong People's Republic of China
Bureau, Chinese Academy of Inspection and Quarantine.
The main drafters of this standard. Chen Xianfeng, Zhang Huili, Chending Hu, Zhang Ji Hong, Xu Ying, Guo Lixin, Wen Weigang, Yang Hongxia, Du Hongzhong, Shan Wu products.
Plum pox virus quarantine and identification methods
1 Scope
This standard specifies the immunology and molecular biology quarantine and identification of plum pox virus.
This standard applies to plant material, including quarantine and identification of vegetative propagation material, tissue culture, seeds, flowers and fruits of plum pox virus.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use - Specification and test methods GB/T 6682 Analysis
SN/T 1840 plant virus immune electron microscopy method
3 Equipment and Reagents
3.1 Equipment
Ordinary PCR, real-time PCR instrument, microplate reader, electronic balance (1/1000g), electrophoresis, electrophoresis tank, gel imaging system, water
Bath, high-speed desktop refrigerated centrifuge, -80 ℃ ultra-low temperature freezer, clean benches, autoclaves, ice maker, microwave, vortex
Device. Adjustable micropipette (2μL, 10μL, 100μL, 200μL, 1000μL, 5000μL) and the corresponding RNase-free tip, no
RNase centrifuge tubes, PCR tubes and mortar and the like.
3.2 Reagents
Unless otherwise specified, all reagents were of analytical grade experiment, test water should be consistent with GB/T 6682 in the relevant regulations.
The Trizol, diethyl pyrocarbonate (of DEPC), liquid nitrogen, chloroform, isopropanol, ethanol, [beta] -mercaptoethanol, RNA reverse transcriptase,
TaqDNA polymerase, SYBRgreenI, DNA molecular weight standard, ethidium bromide, agarose, bromophenol blue. Double antibody enzyme
Linked immunosorbent assay method (DAS-ELISA) detection reagent preparation method, see Appendix B, B.1.
4 identification of quarantine
4.1 Symptom Check
Carefully check the plant leaves and flowers, fruit, whether stone faded, ringed, deformities and other suspicious symptoms, if there is a typical symptoms (cf.
See Appendix A), can be collected showing symptoms of flower, leaf or fruit subsequent testing.
4.2 pairs of antibody ELISA method (DAS-ELISA)
DAS-ELISA specific steps in Appendix B.
4.3 Common RT-PCR detection method
Common RT-PCR method to detect the specific steps in Appendix C.
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