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GB/T 31808-2015 English PDF

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GB/T 31808-2015: Detection and identification of Albugo tragopogonis (Pers.) S. F. Gray
Status: Valid
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GB/T 31808-2015English239 Add to Cart 3 days [Need to translate] Detection and identification of Albugo tragopogonis (Pers.) S. F. Gray Valid GB/T 31808-2015

PDF similar to GB/T 31808-2015


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Basic data

Standard ID GB/T 31808-2015 (GB/T31808-2015)
Description (Translated English) Detection and identification of Albugo tragopogonis (Pers.) S. F. Gray
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 65.020.01
Word Count Estimation 11,153
Date of Issue 2015-07-03
Date of Implementation 2015-11-27
Regulation (derived from) National Standard Announcement 2015 No.22
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the method for inspection and identification of sunflower white rust. This standard applies to sunflower seeds and sunflower plants quarantine and identification of white rust.

GB/T 31808-2015: Detection and identification of Albugo tragopogonis (Pers.) S. F. Gray

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Albugo tragopogonis (Pers.) SF Gray ICS 65.020.01 B16 National Standards of People's Republic of China Sunflower White Rust quarantine and identification methods Issued on. 2015-07-03 2015-11-27 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. Xinjiang People's Republic of China Exit Inspection and Quarantine, the Xinjiang Production and Construction Corps Agricultural Technology Extension Station. The main drafters. Zhang Xianglin, Wang Chong, Zhaobing Mei, Wang Wen-cheng, Zhangjiang Guo, Ke Yi dry. Sunflower White Rust quarantine and identification methods

1 Scope

This standard specifies the method for inspection and identification of sunflower white rust. This standard applies to sunflower seeds and sunflower plants quarantine and identification of white rust.

2 apparatus and appliances

Biological microscope, stereo microscope, clean benches, autoclave, high-speed centrifuges, ice maker, full warm bath, PCR amplification, Electrophoresis, gel imager, reciprocating oscillator, petri dishes, beakers, alcohol lamps, flasks, slides, cover slips, tweezers, scissors, measuring straw.

3 Reagents

Lactic acid, phenol, glycerol, cotton blue, saffron, sodium hypochlorite (NaClO), sodium dodecyl sulfate (SDS), Tris - salt Acid (Tris-HCl), magnesium chloride (MgCl2), ethylenediaminetetraacetic acid tetrasodium salt (EDTA), sodium hydroxide (NaOH), sodium acetate (NaOAc), Potassium chloride (KCl), cetyl trimethyl ammonium bromide (CTAB), ethanol (Ethanol), phenol (Phenol), chloroform (Chloro- form), isopropyl alcohol (Isopropanol), iso-amyl alcohol (Isoamylalcohol), nucleic acid dye (SYBRGreenⅠ), proteinase K, Taq DNA polymerase (Taq DNApolymerase), deoxyribonucleoside triphosphates (dNTP), DNA molecular weight marker (DNA Marker), TAE electrophoresis buffer (TAEbuffer).

4 identification of quarantine

4.1 Field Symptom Check In the mid-squaring to flowering sunflower to sunflower cultivation areas or isolated field investigation, observed incidence of sunflower plants. 4.2 seed infection inspection Select 5g deflated small sunflower seeds, placed in a clean flask, was added 200mL of water and a small amount of detergent, in the reciprocating oscillator The oscillation 10min, tossed away the washing liquid, then add the same amount of water, washing was repeated 3 times to 5 times, until the Seed Sunflower seeds washed on (If there is no seed coating, you can omit this step), dried, peeled sunflower seeds, the pericarp and seeds together into a beaker containing milk phenol oil, the wine Fine lamp boil 3min, after cooling dissecting microscope with tweezers stripping endocarp and seed coat, placed in a clean, dish, add a small amount of cotton blue - Saffron staining solution, standing around 5h, after washing with 95% ethanol, picked up with tweezers inner peel or seed coat placed on a slide, add a drop of milk phenol Floating oil as a carrier, the cover sheet microscopy. 4.3 Pathogen morphology With a clean surface of leaf blade scraping a small amount of sunflower disease (or petiole, disk, etc.) white blister plaque bacteria, placed on a slide in the raw Was observed under a microscope the morphological characteristics of bacteria, cysts record stems, sporangium, spore shape and size of the eggs. The inner sunflower seed coat or pericarp prepared slide and placed under observation bacteria form characteristic biological microscope, recording cysts stems, sporocysts, eggs The shape and size of the spores.

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