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GB/T 31798-2015 English PDF

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GB/T 31798-2015: Detection and identification of Plenodomus biglobosus (Shoemaker and H. Brun) Gruyter, Aveskamp and Verkley
Status: Valid
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GB/T 31798-2015English319 Add to Cart 3 days [Need to translate] Detection and identification of Plenodomus biglobosus (Shoemaker and H. Brun) Gruyter, Aveskamp and Verkley Valid GB/T 31798-2015

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Basic data

Standard ID GB/T 31798-2015 (GB/T31798-2015)
Description (Translated English) Detection and identification of Plenodomus biglobosus (Shoemaker and H. Brun) Gruyter, Aveskamp and Verkley
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 65.020.01
Word Count Estimation 15,116
Date of Issue 2015-07-03
Date of Implementation 2015-11-27
Regulation (derived from) National Standard Announcement 2015 No.22
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 31798-2015: Detection and identification of Plenodomus biglobosus (Shoemaker and H. Brun) Gruyter, Aveskamp and Verkley


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Detection and identification of Plenodomus biglobosus (Shoemaker ICS 65.020.01 B16 National Standards of People's Republic of China Rapeseed quarantine and identification of Phytophthora (Shoemaker Issued on. 2015-07-03 2015-11-27 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. People's Republic of China Hubei Exit Inspection and Quarantine, Chinese Academy of Inspection and Quarantine, the People's Republic of China And Shanghai, China Entry-Exit Inspection and Quarantine of Jiangsu People's Republic of China, Central China Agricultural University. The main drafters of this standard. Zhao Hui, Wang Zhenhua, Cengxian Dong, Yi Jianping, Li Bin, Li Guoqing, Wu Shan goods, Cai Xiang. Rapeseed quarantine and identification of Phytophthora

1 Scope

This standard specifies the rapeseed Phytophthora (Plenodomusbiglobosus) of quarantine and identification methods. This standard applies to rapeseed, oilseed rape plants and their products in Phytophthora quarantine and identification.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis

3 Equipment and Reagents

3.1 Equipment Biological microscope, stereo microscope, high-speed refrigerated centrifuge, water meter, clean bench, sterilizer, ice maker, PCR instrument, into a gel Imaging systems, electrophoresis, incubators, ultra-low temperature freezer, a conventional refrigerator, a vortex. 3.2 Reagents Unless otherwise specified, all reagents were of analytical grade or biochemical reagents, test water line with GB/T 6682. 3.2.1 1% sodium hypochlorite 3.2.2 DNA Extraction Reagent. DNA extraction kit, isopropanol, ethanol. Urea extract (7mol/LUrea, 50mmol/L Tris-HClpH8.0,62.5mmol/LNaCl, 1% SDS), proteinase K (20mg/mL), phenol. chloroform. isoamyl alcohol (25.24.1) solution, 3mol/LNaAc (pH5.2). 3.2.3 PCR Reagents. 10 × PCR buffer (containing 25mmol/LMgSO4), Taq enzyme, dNTPs. 3.2.4 Gel Electrophoresis Reagents. agarose, TAE, Loading buffer, EB. 3.2.5 20% V8 medium. V8 vegetable juice (CampbelSoup company) 200mL, CaCO30.75g, agar 13.0g, distilled Water volume to 1000mL, adjusted to pH 5.8,121 ℃ autoclave 20min. 3.2.6 potato dextrose agar (PDA). Potato extract powder 5.0g, glucose 20.0g, chloramphenicol 0.1g, agar 13.0g, evaporated Distilled water volume to 1000mL, adjusted to pH 5.6,121 ℃ autoclave 20min. Identification of bacteria 4.1 Symptom Check 4.1.1 Seed Symptom Check Take a sample amount of 1% to 5% (if less may be appropriate to increase the proportion of the sample) seeds, dissecting microscope to pick wrinkled, shriveled, discolored, torn Moldy grain or seed or plant debris, each seed sample selected is not less than 500, with a separation of pathogens. Another 1kg seed,

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