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GB/T 31806-2015 English PDF

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GB/T 31806-2015: Detection and identification of Potato virus V
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Basic data

Standard ID GB/T 31806-2015 (GB/T31806-2015)
Description (Translated English) Detection and identification of Potato virus V
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 65.020.01
Word Count Estimation 13,125
Date of Issue 2015-07-03
Date of Implementation 2015-11-27
Quoted Standard SN/T 2122
Regulation (derived from) National Standard Announcement 2015 No.22
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This Standard specifies the phytosanitary quarantine and identification of potato virus V. This Standard applies to the potato tubers, potato plantlets and other reproductive material in the quarantine and identification of V virus.

GB/T 31806-2015: Detection and identification of Potato virus V

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Potato virus V ICS 65.020.01 B16 National Standards of People's Republic of China Quarantine and identification of potato virus V Issued on. 2015-07-03 2015-11-27 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Yunnan Exit Inspection and Quarantine. The main drafters of this standard. MATURAL, Min Li, Ma Jie, Lu Jie, Zhang Yongjiang, Jun hole, Weimei Sheng. Quarantine and identification of potato virus V

1 Scope

This standard specifies the phytosanitary quarantine and identification of potato virus V. This standard applies to quarantine and identification of potato tubers, potato plantlets and other reproductive material V virus.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. SN/T 2122 entry and exit quarantine of plants and plant products sampled

3 equipment and reagents

3.1 Equipment Microplate reader, the balance (a sense of volume 1/10000g), pH meter, trace juicer, PCR, real-time PCR instrument, electrophoresis, electrophoresis tank, Gel imaging system, isolate the greenhouse, water bath, cold refrigerator. Micropipette (2μL, 10μL, 20μL, 100μL, 200μL, 1000μL), ELISA plate, mortar tubes, flower pots, sterilization Soil. 3.2 Reagents Enzyme-linked immunosorbent assay reagent see Appendix B, RT-PCR detection reagent in Appendix C, real-time fluorescent RT-PCR detection reagent see Appendix D, biological assay reagent see Appendix E.

4 Sampling and Sample Preparation

4.1 Sampling Sampling conducted in accordance with the provisions of SN/T 2122's. 4.2 Preparation of the samples of potato tubers Potato tubers sown in sterilized soil, grown and symptoms observed at 25 ℃. Grow to be about 10 after showing symptoms of leaf explants Line numbers do not show symptoms of the plant grouping (for a group of 10) and number. Leaves collected for enzyme-linked immunosorbent assay, RT-PCR detection, real Fluorescent RT-PCR, and biological assays. Sample preparation 4.3 Tissue Culture The tissue culture bottle of each plant were sampled, the sampled tissue culture bottle product as a sample for the ELISA test sample Fixed, by RT-PCR, real-time fluorescence RT-PCR, and biological assays.

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