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GB/T 31796-2015 English PDF

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GB/T 31796-2015: Detection and identification of Fusarium tucumaniae and Fusarium virguliforme
Status: Valid
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GB/T 31796-2015English399 Add to Cart 4 days [Need to translate] Detection and identification of Fusarium tucumaniae and Fusarium virguliforme Valid GB/T 31796-2015

PDF similar to GB/T 31796-2015


Standard similar to GB/T 31796-2015

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Basic data

Standard ID GB/T 31796-2015 (GB/T31796-2015)
Description (Translated English) Detection and identification of Fusarium tucumaniae and Fusarium virguliforme
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 65.020.010
Word Count Estimation 19,187
Date of Issue 2015-07-03
Date of Implementation 2015-11-27
Regulation (derived from) National Standard Announcement 2015 No.22
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the South American soybean sudden death syndrome, sudden death syndrome of soybean germs and North America quarantine and identification of bacteria. This standard applies to soybeans and soybean grain field soil and plant debris carried by South Africa, North America soybean sudden death syndrome pathogen identification.

GB/T 31796-2015: Detection and identification of Fusarium tucumaniae and Fusarium virguliforme

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Detection and identification of Fusarium tucumaniae and Fusarium virguliforme ICS 65.020.010 B16 National Standards of People's Republic of China South American soybean sudden death syndrome of soybean germs and North America SIDS quarantine and identification of bacteria Issued on. 2015-07-03 2015-11-27 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. Chinese Academy of Inspection and Quarantine, Shenzhen People's Republic of China Exit Inspection and Quarantine. Drafters of this standard. Wu Shan goods, Wang Ying, Zhang Guiming, Chen Chi-nan. South American soybean sudden death syndrome of soybean germs and North America SIDS quarantine and identification of bacteria

1 Scope

This standard specifies the South American soybean sudden death syndrome, sudden death syndrome of soybean germs and North America quarantine and identification of bacteria. This standard applies to soybeans and soybean grain field soil and plant debris carried by South Africa, North America soybean sudden death syndrome pathogen identification.

2 apparatus and appliances

2.1 Instrument Biological microscope, clean benches, light incubator, electronic scales (1/1000g), autoclaves, refrigerators, PCR amplification, fluorescence PCR amplification, electrophoresis, gel imager, high-speed refrigerated centrifuge, heated water bath, shaker. 2.2 Appliances Beakers, flasks, graduated cylinder, test tubes, petri dishes, alcohol lamp, tweezers, scissors, scalpels, needle inoculation, slides, cover slips, plastic pestle Pipettes, pipette tips, centrifuge tubes, liquid nitrogen tank.

3 Reagents and culture medium

3.1 Reagents Agar, potato, glucose, lactate, agarose, sterile double distilled water, liquid nitrogen, peptone, potassium dihydrogen phosphate, magnesium sulfate, streptomycin, ADM, PCNB, chlortetracycline, rifampicin, Tris-HCl, MgCl2, HCl, EDTA, NaOH, NaOAc, KCl, Tris, CTAB, TAE, absolute ethanol (Ethanol), phenol (Phenol), ethidium bromide (EB), chloroform (Chloroform), isopropanol (Isopropanol), iso-amyl alcohol (Isoamylalcohol), proteinase K, TaqDNA polymerase, dNTP, DNA molecular weight standards Was double PCR primers Fu1/2 and Fsg-α-1/2, real-time PCR primers and probes 16-FP/16-RP and 16-AG, TaqManUniversalPCRMasterMix. 3.2 Medium 0.2% agar aqueous solution, PCNB for a selective medium, potato dextrose medium (PDA), a synthetic medium low nutrient (SNA). See Appendix A. specific formula

4 identification of quarantine

4.1 Symptom Check North and South America soybean sudden death syndrome on the symptoms are not very different, disease symptoms are typical brown necrotic leaf mesophyll, veins sometimes Paul Holding green roots of vascular tissue with brown lesions, white pith. See Appendix A description of the symptoms of disease. The roots of soybean plants suspected symptoms collected in the field, the soil around the plant and set aside. Screening carrying soil in soybean grains, and pick Choose one mixed plant debris, especially soybeans underground parts, spare.

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