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GB/T 31803-2015 English PDF

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GB/T 31803-2015: Detection and identification of Cotton leaf crumple virus
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GB/T 31803-2015English259 Add to Cart 3 days [Need to translate] Detection and identification of Cotton leaf crumple virus Valid GB/T 31803-2015

PDF similar to GB/T 31803-2015


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Basic data

Standard ID GB/T 31803-2015 (GB/T31803-2015)
Description (Translated English) Detection and identification of Cotton leaf crumple virus
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 65.020.01
Word Count Estimation 12,17
Date of Issue 2015-07-03
Date of Implementation 2015-11-27
Regulation (derived from) National Standard Announcement 2015 No.22
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This Standard specifies the immunology and molecular biology methods such as quarantine and identification of wrinkled cotton leaf virus. This Standard applies to quarantine and identification may carry wrinkled cotton leaf virus in vivo tissue of host plant leaves.

GB/T 31803-2015: Detection and identification of Cotton leaf crumple virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Cotton leaf crumple virus ICS 65.020.01 B16 National Standards of People's Republic of China Wrinkled cotton leaf virus quarantine and identification methods Issued on. 2015-07-03 2015-11-27 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Distribution of this document Institutions do not assume the responsibility to identify these patents. This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points. This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Fujian Exit Inspection and Quarantine, Zhejiang University, Zhejiang Jiang CIQ. The main drafters. Zhang Yongjiang, Wing Lei, Shen Jianguo, Zhou Xueping, Lu Jie, Zhang Mingzhe, MATURAL, Zhu Shuifang. Wrinkled cotton leaf virus quarantine and identification methods

1 Scope

This standard specifies the immunology and molecular biology methods such as quarantine and identification of wrinkled cotton leaf virus. This standard applies to quarantine and identification may carry wrinkled cotton leaf virus in vivo tissue of host plant leaves. 2 equipment, appliances and reagents 2.1 Equipment Electronic analytical balance (0.001g), a small centrifuge, refrigerated centrifuge desktop, heated water bath, the Reader, general PCR, quantitative fluorescence Light PCR, electrophoresis system, pH meter, gel imaging system, 4 ℃ refrigerator, clean benches, -80 ℃ ultra-low temperature refrigerators, Autoclave, Ice maker, a vortex, and microwave ovens. 2.2 Appliances Adjustable pipette (2.5μL, 10μL, 20μL, 100μL, 1000μL), pipette tips, centrifuge tubes, Eppendorf tubes (0.2mL, 0.5mL, 1.5mL) and mortar and the like. 2.3 Reagents Unless otherwise stated, all test reagents were analytical grade or biochemical reagents. DAS-ELISA, conventional PCR and real-time PCR detection reagent, respectively, see Appendix B, Appendix C and Appendix D. Quarantine and identification of 3 3.1 DAS-ELISA detection DAS-ELISA detected in Appendix B. 3.2 conventional PCR detection Conventional PCR detection Appendix C. 3.3 real-time PCR detection Real-time PCR assay in Appendix D. Analyzing the results of 4 Results 3.1, 3.2 and 3.3 in two different principles of detection methods were positive, the sample can be determined as CLCrV positive. Generally DAS-ELISA test is positive, the PCR or conventional real-time PCR test result is positive samples CLCrV can be judged positive.

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