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GB/T 30989-2014 English PDF

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GB/T 30989-2014: Technical regulation of high-throughput gene sequencing
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PDF similar to GB/T 30989-2014


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Basic data

Standard ID GB/T 30989-2014 (GB/T30989-2014)
Description (Translated English) Technical regulation of high-throughput gene sequencing
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard G04
Classification of International Standard 07.080
Word Count Estimation 18,141
Date of Issue 7/24/2014
Date of Implementation 3/1/2015
Quoted Standard GB/T 6682; GB/T 27025-2008; JB/T 6777; YY/T 0087; YY 0569; YY/T 0657; YY 91037; ASTM E1342-1997
Regulation (derived from) National Standards Bulletin No. 19, 2014
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies requirements for the terms and definitions of high-throughput gene sequencing techniques, principle sequencing Technical indicators related to the main instruments and sequencing reagent preparation and sequencing methods. This sta

GB/T 30989-2014: Technical regulation of high-throughput gene sequencing

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Technical regulation of high-throughput gene sequencing ICS 07.080 G04 National Standards of People's Republic of China Technical specification for high-throughput sequencing genes Issued on. 2014-07-24 2015-03-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard by the Shenzhen Science and Technology Co., Ltd. made kang gene. This standard biochemical tests by the National Standardization Technical Committee (SAC/TC387) centralized. This standard was drafted. kang gene Shenzhen Technology Co., Ltd., Shenzhen, Hong Technology Research Institute, because high-throughput biological, Tianjin Rui Hong Xin Gene Technology Co., Ltd., the National Human Genome Center at Southern Research, Beijing Institute of Biophysics, Chinese Academy of Sciences, Shenzhen City of Standards and Technology Research Institute, Shanghai Jiaotong University Affiliated Ruijin Hospital. The main drafters of this standard. Division of Sheng Tong, Tan Huibiao, Jin Hong, Zhao Guoping, Wang Sheng Yue, Chan Yun Sang, Zhou, Zhang. Technical specification for high-throughput sequencing genes

1 Scope

This standard specifies the terms and definitions of high-throughput gene sequencing techniques, principle sequencing Technical indicators related to the main instrument, sequencing reagents And it requires preparation and sequencing methods. This standard applies to animal tissues, blood, feces, oral mucosa, hair, plant tissue, soil, microorganisms and other biological samples high Throughput gene sequencing. This standard applies to unidirectional sequencing, direct sequencing, sequencing of the three types of mixed sample sequencing, expression profiling also apply to sequencing, ChIP test Sequence, Cancer Research and parting, miRNA sequenced microbial genomes element, the type of pathogen detection, immunological and immunological diseases work, methylated Sequencing, sequence polymorphism detection, clinical genetic diagnosis, personalized sequencing and genome sequencing.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis General requirements GB/T 27025-2008 testing and calibration laboratories JB/T 6777 UV-Vis spectrophotometer YY/T 0087 electrophoresis apparatus YY0569 Ⅱ level biological safety cabinet YY/T 0657 Medical Centrifuge YY91037 electric heated water bath ASTME1342-1997 Save bacteria, fungi with freezing, freeze-drying and low temperature curing method, protists, viruses, genetic factors And standard animal and plant tissues Practice (StandardPracticeforPreservationbyFreezing, Freeze-Drying, andLowTemperatureMaintenanceofBacteria, Fungi, Protista, Viruses, GeneticElements, andAn- imalandPlantTissues)

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Gene gene Located on chromosome encodes a specific functional product (e.g. protein or RNA molecule) of a nucleotide sequence, genetic information is basic unit. 3.2 Genome genome A living body has a sum of all of the genetic information. 3.3 Nucleotide nucleotide Constitute the basic unit of nucleic acids, it consists of three parts. the nitrogenous base pentose phosphate and cyclic.

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