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GB/T 30988-2014 English PDF

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GB/T 30988-2014: Methods of genomic DNA extraction from plants with high levels of polyphenols
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Basic data

Standard ID GB/T 30988-2014 (GB/T30988-2014)
Description (Translated English) Methods of genomic DNA extraction from plants with high levels of polyphenols
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B30
Classification of International Standard 07.080
Word Count Estimation 8,889
Date of Issue 7/24/2014
Date of Implementation 3/1/2015
Quoted Standard GB/T 6682-2008; GB/T 19495.3-2004
Regulation (derived from) National Standards Bulletin No. 19, 2014
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the order represented by the tea polyphenols plant genomic DNA extraction and purification methods and DNA purity and concentration of the test method. This standard applies to tea, potatoes, cherries, grapes and other plant genome

GB/T 30988-2014: Methods of genomic DNA extraction from plants with high levels of polyphenols

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Methods of genomic DNA extraction from plants with high levels of polyphenols ICS 07.080 B30 National Standards of People's Republic of China Polyphenolic plant genomic DNA extraction and purification And test methods Issued on. 2014-07-24 2015-03-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Table of Contents

Introduction Ⅲ 1 Scope 1 2 Normative references 1 3 Abbreviations 1 4 Principle 1 5 Laboratory 2 General requirements 6 reagent solution 2 7 2 instruments and equipment 8 extraction step 3 9 DNA purity and concentration test 4

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. The standard proposed by China Testing Technology Research Institute. This standard biochemical tests by the National Standardization Technical Committee (SAC/TC387) centralized. This standard was drafted. China Testing Technology Research Institute, measured test Technology Co., Ltd. The main drafters of this standard. Tan peace, Shen Xing, Tang Xiangkai, Zhou Hua, Sun Dengfeng. Polyphenolic plant genomic DNA extraction and purification And test methods

1 Scope

This standard specifies the order represented by the tea polyphenols plant genomic DNA extraction and purification methods and DNA purity and concentration test method. This standard applies to tea, potatoes, cherries, grapes and other plant genome rich in polyphenols and plant DNA extraction purification.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 6682-2008 analytical laboratory use specifications and test methods GB/T 19495.3-2004 GMO detection of nucleic acid extraction and purification method

3 Abbreviations

The following abbreviations apply to this document. Tris. Tris (hydroxymethyl) aminomethane SDS. sodium dodecyl sulfate PVP. polyvinylpyrrolidone RNaseA. ribonuclease A FF. Xylene Green EDTA. ethylenediaminetetraacetic acid TE. Tris (hydroxymethyl) aminomethane - EDTA Principle 4 4.1 Extraction and Purification In liquid nitrogen, and broken polyphenols of plant leaves by physical grinding methods cells use antioxidants polyphenols prevent oxygen Browning, while taking advantage of polyvinylpyrrolidone (PVP) to remove the adhesive properties of polyphenolic compounds and other secondary metabolites and other impurities, then Lee By means of chemical DNA released from the plant cells, and then discarded DNA in protein and other impurities, as well as DNA extraction process The organic solvent is added iso-amyl alcohol, isopropanol, ethanol, etc., and finally get pure DNA. 4.2 Purity Test In pH7 ~ 8.5 under, according to the DNA absorption peak at 260nm, protein absorption peak at 280nm at thiocyanate, Carbohydrates (sugars) in the absorption peak at 230nm, phenolic substances in the characteristic absorption peak at 270nm using the A260/A280,

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