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GB/T 30986-2014 English PDF

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GB/T 30986-2014: Method for the determination of glucose, sucrose, maltose contents in biochemical products -- Liquid chromatography with refractive index detector
Status: Valid

Standard ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status	PDF
GB/T 30986-2014	English	189	Add to Cart	3 days [Need to translate]	Method for the determination of glucose, sucrose, maltose contents in biochemical products -- Liquid chromatography with refractive index detector	Valid	GB/T 30986-2014

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Basic data

Standard ID	GB/T 30986-2014 (GB/T30986-2014)
Description (Translated English)	Method for the determination of glucose, sucrose, maltose contents in biochemical products -- Liquid chromatography with refractive index detector
Sector / Industry	National Standard (Recommended)
Classification of Chinese Standard	G85
Classification of International Standard	07.080
Word Count Estimation	8,898
Date of Issue	7/24/2014
Date of Implementation	3/1/2015
Quoted Standard	GB/T 6682
Regulation (derived from)	National Standards Bulletin No. 19, 2014
Issuing agency(ies)	General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary	This standard specifies the refractive index liquid chromatography method for the determination of biochemical products of glucose, sucrose, maltose content approach. This standard applies to sugar pepsin, intravenous human immunoglobulin, human rabies im

GB/T 30986-2014: Method for the determination of glucose, sucrose, maltose contents in biochemical products -- Liquid chromatography with refractive index detector

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Method for the determination of glucose, sucrose, maltose contents in biochemical products. Liquid chromutography with refractiveindex detector ICS 07.080 G85 National Standards of People's Republic of China Biochemical products glucose, sucrose, maltose Determination of Liquid Chromatography Refractive Index Detector Methodforthedeterminationofglucose, sucrose, maltosecontentsin Issued on. 2014-07-24 2015-03-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Introduction Ⅲ 1 Scope 1 2 Normative references 1 Method summary 3 1 4 Reagents and solutions 1 Instruments and equipment 5 1 Sample Preparation 6 2 7 Procedure 2 8 calculation results 2 Appendix A (informative) glucose, sucrose, maltose reference material chromatograms 4

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. The standard proposed by the Chengdu University. This standard biochemical tests by the National Standardization Technical Committee (SAC/TC387) centralized. This standard was drafted. Chengdu University of Traditional Chinese Medicinal Chemistry Laboratory, China Testing Technology Research Institute. The main drafters of this standard. Gou Xiao Jun, Tan peace, Yan Jun, Sun Dengfeng, Xie Jian Zhen. Biochemical products glucose, sucrose, maltose Determination of Liquid Chromatography Refractive Index Detector

1 Scope

This standard specifies the refractive index liquid chromatography method for the determination of biochemical products of glucose, sucrose, maltose content approach. This standard applies to sugar pepsin, intravenous human immunoglobulin, human rabies immune globulin and other biochemical products grapes Sugar, sucrose, maltose determination.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis

3 Method summary

The sample was dissolved in water and heated in addition to protein, centrifuged and the supernatant with the volume fraction of 40% aqueous acetonitrile solution volume to volume, the 0.45μm membrane filter, by liquid chromatography refractive index detector measurement, the retention time qualitative, external standard.

4 Reagents and solutions

4.1 Unless otherwise specified, the reagents used were of analytical grade water as a water GB/T 6682 regulations. 4.2 acetonitrile. chromatography. 4.3 volume fraction of 40% aqueous acetonitrile solution. Take 40mL acetonitrile HPLC grade water was mixed with 60mL. 4.4 glucose (CAS Number. 50-99-7) Standard. Purity ≥99%. 4.5 Sucrose (CAS Number. 57-50-1) Standard. Purity ≥99%. 4.6 maltose (CAS Number. 69-79-4) Standard. Purity ≥99%. Sugar 4.7 standard stock solution. Weigh 5g (accurate to 0.0001g) by vacuum drying to constant weight of glucose, sucrose, maltose standards, Plus the amount of the volume fraction of 40% acetonitrile in water to dissolve, each transferred to a 100mL volumetric flask, with the volume fraction of 40% aqueous acetonitrile to volume To the mark. Sugar stock standard solution concentration of 50.0mg/mL. Sugar 4.8 standard solution. Imbibe sugar were standard stock solution 0.5mL, 1.0mL, 2.5mL, 5.0mL, 7.5mL, 10.0mL to 50mL volumetric flask, dilute to the mark with the volume fraction of 40% aqueous acetonitrile. Prepared concentrations were 0.5mg/mL, 1.0mg/mL, 2.5mg/mL, 5.0mg/mL, 7.5mg/mL, glucose 10.0mg/mL, the standard solution of sucrose and maltose.

5 instruments and equipment

5.1 High performance liquid chromatograph. with a differential refractive index detector. 5.2 Analytical balance, a sense of the amount of 0.1mg. 5.3 high-speed centrifuge, 10000r/min.

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