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GB/T 29664-2013: Determination of vitamin B3 (Nicotinic acid and Nicotinamide) in cosmetics -- HPLC and HPLC-MS/MS
Status: Valid
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| GB/T 29664-2013 | English | 279 |
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Determination of vitamin B3 (Nicotinic acid and Nicotinamide) in cosmetics -- HPLC and HPLC-MS/MS
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GB/T 29664-2013
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Basic data | Standard ID | GB/T 29664-2013 (GB/T29664-2013) | | Description (Translated English) | Determination of vitamin B3 (Nicotinic acid and Nicotinamide) in cosmetics -- HPLC and HPLC-MS/MS | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | Y42 | | Classification of International Standard | 71.100.70 | | Word Count Estimation | 12,175 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Standards Bulletin 2013 No. 17 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies: Cosmetics Vitamin B3 (niacin, niacinamide) measurement high performance liquid chromatography and high performance liquid chromatography tandem mass spectrometry. This standard applies to: cream, lotion, lotion, soap, such as beau | GB/T 29664-2013: Determination of vitamin B3 (Nicotinic acid and Nicotinamide) in cosmetics -- HPLC and HPLC-MS/MS
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Determination of vitamin B3 (Nicotinic acid and Nicotinamide) in cosmetics. HPLC and HPLC-MS/MS
ICS 71.100.70
Y42
National Standards of People's Republic of China
Cosmetics Vitamin B3 (niacin, niacinamide) Determination
HPLC and
High performance liquid chromatography tandem mass spectrometry
DeterminationofvitaminB3 (nicotinicacidandnicotinamide)
Issued on. 2013-09-06
2014-02-15 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Standardization Technical Committee of Fragrance Flavor and Cosmetic (SAC/TC257) centralized.
This standard was drafted. Dalian City Product Quality Supervision, Inspection (National Day of Product Quality Supervision and Inspection Center), Shanghai Daily Chemical
Industrial Research Institute (Fragrance Flavor and Cosmetic National Quality Supervision and Inspection Center), Dalian standard detection technology research center.
The main drafters of this standard. Maoxi Qin, Zheng Shunli Hu Xia, Sun Chih-Ching, Wu Haixia, Pan Wei, Wu Xiao Jian, Kang Wei.
Cosmetics Vitamin B3 (niacin, niacinamide) Determination
HPLC and
High performance liquid chromatography tandem mass spectrometry
1 Scope
This standard specifies a high performance liquid chromatography and high performance liquid chromatography tandem mass cosmetics, vitamin B3 (niacin, niacinamide) measured
Spectroscopy.
This standard applies to creams, lotions, lotions, soaps such as beauty soap, wax-based lipsticks and other cosmetics such as vitamin B3 (niacin, niacinamide)
The quantitative determination of which HPLC niacin, niacinamide detection limits were 2μg/g, the limit of quantification are 6μg/g; HPLC
Chromatography Tandem Mass Spectrometry niacin, niacinamide detection limits were 100μg/kg, the limit of quantification are 300μg/kg.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
Principle 3
Nicotinic acid, nicotinamide polar compound soluble in the aqueous phase, cosmetics soluble components soluble in the organic phase and surface cosmetics
The active agent is enriched in the organic phase and the aqueous phase at the interface. Use of water and dichloromethane (or isooctane) dual liquid system to target and cosmetics
Preliminary separation soluble components and surfactants. Under acidic conditions, hybrid solid-phase extraction exchange material enriched by reversed-phase and strong cation
Object, after removal of interfering substances, elution volume, separated by reversed-phase high-performance liquid chromatography, diode array detector (HPLC
Law) or tandem quadrupole mass spectrometry (HPLC-tandem mass spectrometry), external standard calibration curve.
4 Reagents and materials
Unless otherwise specified, the reagents were of analytical grade. Water as a water GB/T 6682 regulations.
4.1 Methanol. chromatographically pure.
4.2 methanol. AR.
4.3 ammonia. AR.
4.4 formic acid. Chromatographic pure.
4.5 formic acid. AR.
4.6 dichloromethane. AR.
4.7 isooctane. AR.
4.8 2% formic acid aqueous solution. Pipette 2mL acid (4.5), diluted with water volume to 100mL.
4.9 2% aqueous ammonia ammoniated methanol. aqueous ammonia 2mL pipette (4.3), diluted with methanol to 100mL.
4.10 1% formic acid aqueous solution. Pipette 1mL acid (4.5), diluted with water volume to 100mL.
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