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Detection and identification of Fusarium oxysporum f. sp - Cubense (E. F. Smith) Snyder and Hansen Race 4
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GB/T 29397-2012
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Basic data | Standard ID | GB/T 29397-2012 (GB/T29397-2012) | | Description (Translated English) | Detection and identification of Fusarium oxysporum f. sp - Cubense (E. F. Smith) Snyder and Hansen Race 4 | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 10,189 | | Regulation (derived from) | National Standards Bulletin 2012 No. 42 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the banana blight FU germs on the 4th race [Fusarium oxysporum f. Sp. Cubense (EFSmith) Snyder & Hansen Race 4] of the on-site inspection and laboratory testing to identify Fusarium oxysporum on the 4th race of Damage symptoms, pat | GB/T 29397-2012: Detection and identification of Fusarium oxysporum f. sp - Cubense (E. F. Smith) Snyder and Hansen Race 4
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Detection and identification of Fusarium oxysporum f. Sp. Cubense (EF Smith) Snyder
ICS 65.020.01
B16
National Standards of People's Republic of China
Fusarium oxysporum Race 4 quarantine detection and identification
f.sp.Cubense (EFSmith) Snyder
Issued on. 2012-12-31
2013-06-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard by the National Standardization Technical Committee of Plant Quarantine (SAC/TC271) and focal points.
This standard was drafted. National Agricultural Technology Extension Service Center, Plant Quarantine Station in Guangdong Province, South China Agricultural University.
The main drafters of this standard. Wang Yuxi, Wang Lin, WU Li-feng, Jiang Zaide, Limin Hui, Ren Xiaoping, Lixiao Ni, Wu Shihao.
Fusarium oxysporum Race 4 quarantine detection and identification
1 Scope
This standard specifies the Fusarium oxysporum Race 4 [Fusariumoxysporumf.sp.Cubense (EFSmith) Snyder
HansenRace4] site inspection and laboratory testing to identify Fusarium oxysporum 4 races Damage symptoms, pathogen morphology, pathogenicity
Assay results colony traits and host range Komada improved medium on the basis of clear-site inspection, laboratory identification, strain
Preservation methods.
This standard applies to bananas (including bananas, plantains, plantain and other Fusarium oxysporum 4 planted susceptible varieties) during the growth of the banana wilt
Germs Races Test No. 4.
Principle 2
According pathogen Fusarium Damage symptoms Race 4, the Ministry of Health to take the junction tissue disease pathogens isolated and cultured according to the pathogen morphology, caused by
Disease resistance measurement results on the suspected pathogen colonies traits Komada improved medium were determined.
3 instruments, appliances and medicines
3.1 equipment, appliances
Instrument. clean benches, incubators, optical microscopes, autoclaves, cameras.
Appliances. shovel, sticks scissors, machetes, paper, oil pen, notebooks, labels, small blade, petri dishes, slides, cover slips, tweezers, a pick, wine
Fine lights.
3.2 Drugs
0.1% mercuric chloride, 70% ethanol, ethanol, sterile water, deionized water, 40% glycerol, dipotassium hydrogen phosphate, potassium chloride, magnesium sulfate heptahydrate, B
Diamine tetra-acetic acid iron sodium, L- asparagine, galactose, agar powder, 75% pentachlorophenol nifedipine, wettable powders, ox bile powder, borax, streptomycin sulfate,
10% phosphoric acid and the like.
4 on-site inspection
4.1 Scene Investigation
4.1.1 The scope of the investigation
Banana plantations and banana plantlets two seedling production and sales venues.
4.1.2 Survey Methods
On Fusarium Race 4 host plants Tacha, to observe the growth condition; to banana growers and managers ask,
OK suspected Fusarium oxysporum Race 4 damage plots, plots of the suspected test site.
4.1.3 identification of symptoms
On the ground portion of plants showing symptoms, cut the roots and stems of vascular prosthesis organizations, to observe whether a color change occurs.
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